Reviewed, UniProtKB/Swiss-Prot Q8NRU9 (KPRS_CORGL)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	Cgl0942, cg1075

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141131
Regions
	Region	223 – 236	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	136	1	Magnesium Potential
	Metal binding	138	1	Magnesium Potential
	Metal binding	147	1	Magnesium Potential
	Metal binding	151	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8NRU9-1 [UniParc]. Last modified October 25, 2002. Version 1. Checksum: D1FD1944CCD9E77A	FASTA	325	35,551
	10 20 30 40 50 60 MTAHWKQNQK NLMLFSGRAH PELAEAVAKE LDVNVTPMTA RDFANGEIYV RFEESVRGSD 70 80 90 100 110 120 CFVLQSHTQP LNKWLMEQLL MIDALKRGSA KRITAILPFY PYARQDKKHR GREPISARLI 130 140 150 160 170 180 ADLMLTAGAD RIVSVDLHTD QIQGFFDGPV DHMHAMPILT DHIKENYNLD NICVVSPDAG 190 200 210 220 230 240 RVKVAEKWAN TLGDAPMAFV HKTRSTEVAN QVVANRVVGD VDGKDCVLLD DMIDTGGTIA 250 260 270 280 290 300 GAVGVLKKAG AKSVVIACTH GVFSDPARER LSACGAEEVI TTDTLPQSTE GWSNLTVLSI 310 320 APLLARTINE IFENGSVTTL FEGEA

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000036 Genomic DNA. Translation: BAB98335.1. BX927150 Genomic DNA. Translation: CAF19649.1.
RefSeq	NP_600170.1. YP_225235.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKR based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	1018934. 3343409.
GenomeReviews	Gene locus Cgl0942 in contig BA000036_GR. Gene locus cg1075 in contig BX927147_GR.
KEGG	cgb:cg1075. cgl:NCgl0905.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NRU9.
OMA	SGRTPIS.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG1075-MON.
BRENDA	2.7.6.1. 812.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_CORGL

Accession

Primary (citable) accession number: Q8NRU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)