Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

HTH-type transcriptional regulator RipA

Gene

ripA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Under iron limitation, RipA negatively controls the expression of the acn (aconitase), catA (catechol 1,2 dioxygenase), leuCD (isopropylmalate dehydratase), narKGHJI (nitrite/nitrate transporter and nitrate reductase), sdhCAB (succinate dehydrogenase), pta (phosphotransacetylase) and katA (catalase) genes. Binds to the consensus sequence in the promoter region.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi113 – 15947H-T-H motifPROSITE-ProRule annotationAdd
BLAST
DNA bindingi165 – 20844H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator RipA1 Publication
Alternative name(s):
AraC-type regulator RipA1 Publication
Repressor of iron proteins A1 Publication
Short name:
RIPA1 Publication
Gene namesi
Name:ripA1 Publication
Ordered Locus Names:Cgl0982, cg1120
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Under iron limitation, the ripA deletion mutant shows a 2-fold higher aconitase activity and a higher mRNA level for the acn, catA, leuCD, narKGHJI, sdhCAB and pta genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331HTH-type transcriptional regulator RipAPRO_0000223239Add
BLAST

Expressioni

Inductioni

Repressed by DtxR under iron excess.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi196627.cg1120.

Structurei

3D structure databases

ProteinModelPortaliQ8NRR3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2207. LUCA.
OMAiHPSECIA.
OrthoDBiEOG683S6N.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51182. SSF51182. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NRR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSASLLWCH SGVSTVRFGE RIFTLVAGDL LFAPEEAQVA DDSQGLVLNI
60 70 80 90 100
RFETLNIMGP ARRIHLGHVW NDRLTFEYSR SLFGKETLSP DIARLFTDRV
110 120 130 140 150
PTPPLPAPRK ARAVAQVLVS NPADQTSLEE FAEIQGVSAR TLQRQFLKST
160 170 180 190 200
GYSFSEWRAA QRVCVAASLL AHDFSISVVA NLVGFAATSS LTRAFRRHTG
210 220 230 240 250
ATPSTFTTGQ IGMGSAGHPP RIPATTTFAE AHQDQQLWIY SGTATVTTPG
260 270 280 290 300
YCRFMGQGDM VTIPAGTQTR IDVAAGSIAF PVPVGLDEWG MDLTRVVAVN
310 320 330
NQQPKPLTIL EQSEWSKLSE ELLNTPVPVQ M
Length:331
Mass (Da):36,045
Last modified:October 1, 2002 - v1
Checksum:i0F3449B8CFED20DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 2813VRFGE…TLVAG → SSVLAREFSLSSQA in AAS92713 (Ref. 3) CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98375.1.
BX927150 Genomic DNA. Translation: CAF19689.1.
AY513595 Genomic DNA. Translation: AAS92713.1.
RefSeqiNP_600210.1. NC_003450.3.
WP_011014026.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98375; BAB98375; BAB98375.
CAF19689; CAF19689; cg1120.
GeneIDi1018972.
KEGGicgb:cg1120.
cgl:NCgl0943.
PATRICi21494018. VBICorGlu203724_0966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98375.1.
BX927150 Genomic DNA. Translation: CAF19689.1.
AY513595 Genomic DNA. Translation: AAS92713.1.
RefSeqiNP_600210.1. NC_003450.3.
WP_011014026.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NRR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg1120.

Protocols and materials databases

DNASUi1018972.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98375; BAB98375; BAB98375.
CAF19689; CAF19689; cg1120.
GeneIDi1018972.
KEGGicgb:cg1120.
cgl:NCgl0943.
PATRICi21494018. VBICorGlu203724_0966.

Phylogenomic databases

eggNOGiCOG2207. LUCA.
OMAiHPSECIA.
OrthoDBiEOG683S6N.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51182. SSF51182. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. "Corynebacterium glutamicum isolate promoter functional region."
    Park S.-D., Park I.-H., Lee S.-N., Lee H.-S.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  4. "The AraC-type regulator RipA represses aconitase and other iron proteins from Corynebacterium under iron limitation and is itself repressed by DtxR."
    Wennerhold J., Krug A., Bott M.
    J. Biol. Chem. 280:40500-40508(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INDUCTION.
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiRIPA_CORGL
AccessioniPrimary (citable) accession number: Q8NRR3
Secondary accession number(s): Q5QHF9, Q6M6H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2002
Last modified: November 11, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.