Q8NRB3 (METE_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Cgl1139, cg1290

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	745 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 745	745	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098627
Sites
	Metal binding	628	1	Zinc By similarity
	Metal binding	630	1	Zinc By similarity
	Metal binding	713	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NRB3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 856E05DE7398ED1F	FASTA	745	81,313
	10 20 30 40 50 60 MTSNFSSTVA GLPRIGAKRE LKFALEGYWN GSIEGRELAQ TARQLVNTAS DSLSGLDSVP 70 80 90 100 110 120 FAGRSYYDAM LDTAAILGVL PERFDDIADH ENDGLPLWID RYFGAARGTE TLPAQAMTKW 130 140 150 160 170 180 FDTNYHYLVP ELSADTRFVL DASALIEDLR CQQVRGVNAR PVLVGPLTFL SLARTTDGSN 190 200 210 220 230 240 PLDHLPALFE VYERLIKSFD TEWVQIDEPA LVTDVAPEVL EQVRAGYTTL AKRDGVFVNT 250 260 270 280 290 300 YFGSGDQALN TLAGIGLGAI GVDLVTHGVT ELAAWKGEEL LVAGIVDGRN IWRTDLCAAL 310 320 330 340 350 360 ASLKRLAARG PIAVSTSCSL LHVPYTLEAE NIEPEVRDWL AFGSEKITEV KLLADALAGN 370 380 390 400 410 420 IDAAAFDAAS AAIASRRTSP RTAPITQELP GRSRGSFDTR VTLQEKSLEL PALPTTTIGS 430 440 450 460 470 480 FPQTPSIRSA RARLRKESIT LEQYEEAMRE EIDLVIAKQE ELGLDVLVHG EPERNDMVQY 490 500 510 520 530 540 FSELLDGFLS TANGWVQSYG SRCVRPPVLF GNVSRPAPMT VKWFQYAQSL TQKHVKGMLT 550 560 570 580 590 600 GPVTILAWSF VRDDQPLATT ADQVALALRD EINDLIEAGA KIIQVDEPAI RELLPLRDVD 610 620 630 640 650 660 KPAYLQWSVD SFRLATAGAP DDVQIHTHMC YSEFNEVISS VIALDADVTT IEAARSDMQV 670 680 690 700 710 720 LAALKSSGFE LGVGPGVWDI HSPRVPSAQE VDGLLEAALQ SVDPRQLWVN PDCGLKTRGW 730 740 PEVEASLKVL VESAKQAREK IGATI

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB98532.1. BX927151 Genomic DNA. Translation: CAF19845.1.
RefSeq	NP_600367.1. NC_003450.3. YP_225431.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NRB3.
ModBase	Search...
2D gel databases
World-2DPAGE	0001:Q8NRB3.
Proteomic databases
PRIDE	Q8NRB3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019124. 3342567.
GenomeReviews	Gene locus Cgl1139 in contig BA000036_GR. Gene locus cg1290 in contig BX927147_GR.
KEGG	cgb:cg1290. cgl:NCgl1094.
PATRIC	21494322. VBICorGlu203724_1118.
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q8NRB3.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1290-MONOMER. MetaCyc:MONOMER-9441.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CORGL

Accession

Primary (citable) accession number: Q8NRB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program