Reviewed, UniProtKB/Swiss-Prot Q8NR40 (GLGB_CORGL)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Cgl1224, cg1381

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188699
Sites
	Active site	303	1	By similarity
	Active site	338	1	By similarity
	Active site	343	1	By similarity
	Active site	406	1	By similarity
	Active site	408	1	By similarity
	Active site	461	1	By similarity
	Active site	528	1	By similarity
	Active site	529	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NR40-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: EE6BBEB3B0EC80F9	FASTA	731	82,593
	10 20 30 40 50 60 MTVDPASHIT IPEADLARLR HCNHHDPHGF YGWHETEAGS VIRTRQVGAT QVNLLIDDTS 70 80 90 100 110 120 HVMTPIGDDI FAIDLGHRER ADYRLEVTWP DQEPQVKADP YYFLPTVGEM DIYLFSEGRH 130 140 150 160 170 180 ERLWEILGAN IKTYQTALGT VRGTAFTVWA PNAIGCAVVG GFNGWNASQH PMRSMGGSGL 190 200 210 220 230 240 WELFIPGIEE GEVYKFAVQT REGQRRDKAD PMARRAELAP ATGSIVASSE YQWQDSEWLR 250 260 270 280 290 300 ERSQTDLASK PMSVYEVHLG SWRWGKNYED LATELVDYVA DLGYTHVEFL PVAEHPFGGS 310 320 330 340 350 360 WGYQVTGYYA PTSRWGTPDQ FRALVDAFHA RGIGVIMDWV PAHFPKDDWA LARFDGEALY 370 380 390 400 410 420 EHPDWRRGEQ KDWGTLVFDF GRNEVRNFLV ANALYWIEEF HIDGLRVDAV ASMLYLDYSR 430 440 450 460 470 480 EHGEWEPNIY GGRENLEAVQ FLQEMNATVL RLHPGALTIA EESTSWPGVT APTWDGGLGF 490 500 510 520 530 540 SLKWNMGWMH DTLEYFSKNP VHRAFHHSEL TFSLVYAFSE RFVLPISHDE VVHGKGSLWD 550 560 570 580 590 600 RMPGDTWNKA AGLRTFLAYM WSHPGKKLLF MGQEFGQREE WAEGQGLPWD IVDGWQGEYH 610 620 630 640 650 660 EAIRTLTRSL NGVYSDSPAL HTQDFTGEGF TWNKGDDATN NILAFTRFGS DGSQMLCVFN 670 680 690 700 710 720 LSGTSQPEYQ LGVAAGGEWK LVLNTDDAEF LGAENDIATS VQAAATPRDN FAYSLSLHVP 730 AMSAQFYSLQ K

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000036 Genomic DNA. Translation: BAB98617.1. BX927151 Genomic DNA. Translation: CAF19928.1.
RefSeq	NP_600448.1. YP_225514.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	1019207. 3344821.
GenomeReviews	Gene locus Cgl1224 in contig BA000036_GR. Gene locus cg1381 in contig BX927147_GR.
KEGG	cgb:cg1381. cgl:NCgl1177.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NR40.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG1381-MON.
BRENDA	2.4.1.18. 812.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_CORGL

Accession

Primary (citable) accession number: Q8NR40

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)