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Q8NR40 (GLGB_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Cgl1224, cg1381
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7317311,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188699

Sites

Active site4081Nucleophile By similarity
Active site4611Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8NR40 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: EE6BBEB3B0EC80F9

FASTA73182,593
        10         20         30         40         50         60 
MTVDPASHIT IPEADLARLR HCNHHDPHGF YGWHETEAGS VIRTRQVGAT QVNLLIDDTS 

        70         80         90        100        110        120 
HVMTPIGDDI FAIDLGHRER ADYRLEVTWP DQEPQVKADP YYFLPTVGEM DIYLFSEGRH 

       130        140        150        160        170        180 
ERLWEILGAN IKTYQTALGT VRGTAFTVWA PNAIGCAVVG GFNGWNASQH PMRSMGGSGL 

       190        200        210        220        230        240 
WELFIPGIEE GEVYKFAVQT REGQRRDKAD PMARRAELAP ATGSIVASSE YQWQDSEWLR 

       250        260        270        280        290        300 
ERSQTDLASK PMSVYEVHLG SWRWGKNYED LATELVDYVA DLGYTHVEFL PVAEHPFGGS 

       310        320        330        340        350        360 
WGYQVTGYYA PTSRWGTPDQ FRALVDAFHA RGIGVIMDWV PAHFPKDDWA LARFDGEALY 

       370        380        390        400        410        420 
EHPDWRRGEQ KDWGTLVFDF GRNEVRNFLV ANALYWIEEF HIDGLRVDAV ASMLYLDYSR 

       430        440        450        460        470        480 
EHGEWEPNIY GGRENLEAVQ FLQEMNATVL RLHPGALTIA EESTSWPGVT APTWDGGLGF 

       490        500        510        520        530        540 
SLKWNMGWMH DTLEYFSKNP VHRAFHHSEL TFSLVYAFSE RFVLPISHDE VVHGKGSLWD 

       550        560        570        580        590        600 
RMPGDTWNKA AGLRTFLAYM WSHPGKKLLF MGQEFGQREE WAEGQGLPWD IVDGWQGEYH 

       610        620        630        640        650        660 
EAIRTLTRSL NGVYSDSPAL HTQDFTGEGF TWNKGDDATN NILAFTRFGS DGSQMLCVFN 

       670        680        690        700        710        720 
LSGTSQPEYQ LGVAAGGEWK LVLNTDDAEF LGAENDIATS VQAAATPRDN FAYSLSLHVP 

       730 
AMSAQFYSLQ K 

« Hide

References

[1]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000036 Genomic DNA. Translation: BAB98617.1.
BX927151 Genomic DNA. Translation: CAF19928.1.
RefSeqNP_600448.1. NC_003450.3.
YP_225514.1. NC_006958.1.

3D structure databases

ProteinModelPortalQ8NR40.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg1381.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

DNASU3344821.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB98617; BAB98617; BAB98617.
CAF19928; CAF19928; cg1381.
GeneID1019207.
KEGGcgb:cg1381.
cgl:NCgl1177.
PATRIC21494496. VBICorGlu203724_1204.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_CORGL
AccessionPrimary (citable) accession number: Q8NR40
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries