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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei408 – 4081NucleophileUniRule annotation
Active sitei461 – 4611Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Cgl1224, cg1381
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001009 Componenti: Chromosome UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7317311,4-alpha-glucan branching enzyme GlgBPRO_0000188699Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg1381.

Structurei

3D structure databases

ProteinModelPortaliQ8NR40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiQSIAFER.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NR40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVDPASHIT IPEADLARLR HCNHHDPHGF YGWHETEAGS VIRTRQVGAT
60 70 80 90 100
QVNLLIDDTS HVMTPIGDDI FAIDLGHRER ADYRLEVTWP DQEPQVKADP
110 120 130 140 150
YYFLPTVGEM DIYLFSEGRH ERLWEILGAN IKTYQTALGT VRGTAFTVWA
160 170 180 190 200
PNAIGCAVVG GFNGWNASQH PMRSMGGSGL WELFIPGIEE GEVYKFAVQT
210 220 230 240 250
REGQRRDKAD PMARRAELAP ATGSIVASSE YQWQDSEWLR ERSQTDLASK
260 270 280 290 300
PMSVYEVHLG SWRWGKNYED LATELVDYVA DLGYTHVEFL PVAEHPFGGS
310 320 330 340 350
WGYQVTGYYA PTSRWGTPDQ FRALVDAFHA RGIGVIMDWV PAHFPKDDWA
360 370 380 390 400
LARFDGEALY EHPDWRRGEQ KDWGTLVFDF GRNEVRNFLV ANALYWIEEF
410 420 430 440 450
HIDGLRVDAV ASMLYLDYSR EHGEWEPNIY GGRENLEAVQ FLQEMNATVL
460 470 480 490 500
RLHPGALTIA EESTSWPGVT APTWDGGLGF SLKWNMGWMH DTLEYFSKNP
510 520 530 540 550
VHRAFHHSEL TFSLVYAFSE RFVLPISHDE VVHGKGSLWD RMPGDTWNKA
560 570 580 590 600
AGLRTFLAYM WSHPGKKLLF MGQEFGQREE WAEGQGLPWD IVDGWQGEYH
610 620 630 640 650
EAIRTLTRSL NGVYSDSPAL HTQDFTGEGF TWNKGDDATN NILAFTRFGS
660 670 680 690 700
DGSQMLCVFN LSGTSQPEYQ LGVAAGGEWK LVLNTDDAEF LGAENDIATS
710 720 730
VQAAATPRDN FAYSLSLHVP AMSAQFYSLQ K
Length:731
Mass (Da):82,593
Last modified:October 1, 2002 - v1
Checksum:iEE6BBEB3B0EC80F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98617.1.
BX927151 Genomic DNA. Translation: CAF19928.1.
RefSeqiNP_600448.1. NC_003450.3.
WP_011014214.1. NC_006958.1.
YP_225514.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98617; BAB98617; BAB98617.
CAF19928; CAF19928; cg1381.
GeneIDi1019207.
23503227.
KEGGicgb:cg1381.
cgl:NCgl1177.
PATRICi21494496. VBICorGlu203724_1204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98617.1.
BX927151 Genomic DNA. Translation: CAF19928.1.
RefSeqiNP_600448.1. NC_003450.3.
WP_011014214.1. NC_006958.1.
YP_225514.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NR40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg1381.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

DNASUi3344821.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98617; BAB98617; BAB98617.
CAF19928; CAF19928; cg1381.
GeneIDi1019207.
23503227.
KEGGicgb:cg1381.
cgl:NCgl1177.
PATRICi21494496. VBICorGlu203724_1204.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiQSIAFER.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiGLGB_CORGL
AccessioniPrimary (citable) accession number: Q8NR40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 1, 2002
Last modified: May 27, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.