Q8NR20 (DNLJ_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.2

Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	Cgl1244, cg1401

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	680 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity. HAMAP MF_01588
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA ligase (NAD+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 680	680	DNA ligase HAMAP MF_01588		PRO_0000313206
Regions
	Domain	597 – 680	84	BRCT
	Nucleotide binding	35 – 39	5	NAD By similarity
	Nucleotide binding	86 – 87	2	NAD By similarity
Sites
	Active site	113	1	N6-AMP-lysine intermediate By similarity
	Metal binding	408	1	Zinc By similarity
	Metal binding	411	1	Zinc By similarity
	Metal binding	427	1	Zinc By similarity
	Metal binding	433	1	Zinc By similarity
	Binding site	111	1	NAD By similarity
	Binding site	134	1	NAD By similarity
	Binding site	174	1	NAD By similarity
	Binding site	290	1	NAD By similarity
	Binding site	314	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NR20 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: F96FB7AE7F6D01C4	FASTA	680	74,706
	10 20 30 40 50 60 MTEDNAQLRR TWNDLAEKVR YHRDRYYNEQ PEIPDADFDA LFKQLQQLEE DHPELAVPDS 70 80 90 100 110 120 PTMVVGAPVA EQSSFDNVEH LERMLSLDNV FDEQELRDWL GRTPAKQYLT ELKIDGLSID 130 140 150 160 170 180 LVYRNGQLER AATRGDGRVG EDITANARVI EDIPHQLQGT DEYPVPAVLE IRGEVFITVE 190 200 210 220 230 240 DFPEVNAQRI ADGGKPFANP RNAAAGSLRQ KNIEDVKKRR LRMISHGIGF TEGFSPASQH 250 260 270 280 290 300 DAYLALAAWG LPTSPYTEAV TDPEDVVKKV SYWADHRHDA LHEMDGLVIK VDDIASQRAL 310 320 330 340 350 360 GSTSRAPRWA IAYKYPPEEV TTKLLDIQVG VGRTGRVTPF AVMEPVLVAG STVSMATLHN 370 380 390 400 410 420 QSEVKRKGVL IGDTVVIRKA GEVIPEVLGP VVELRDGTER EYIFPTLCPE CGTRLAPAKA 430 440 450 460 470 480 DDVDWRCPNM QSCPGQLSTR LTYLAGRGAF DIEALGEKGA EDLIRTGILL DESGLFDLTE 490 500 510 520 530 540 DDLLSSNVYT TNAGKVNASG KKLLDNLQKS KQTDLWRVLV ALSIRHVGPT AARALAGRYH 550 560 570 580 590 600 SIQALIDAPL EELSETDGVG TIIAQSFKDW FEVDWHKAIV DKWAAAGVTM EEEVGEVAEQ 610 620 630 640 650 660 TLEGLTIVVT GGLEGFTRDS VKEAIISRGG KASGSVSKKT DYVVIGENAG SKATKAEELG 670 680 LRILDEAGFV RLLNTGSADE

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB98637.1. BX927151 Genomic DNA. Translation: CAF19947.1.
RefSeq	NP_600467.1. NC_003450.3. YP_225533.1. NC_006958.1.
3D structure databases
HSSP	HSSP built from PDB template 1ZAU based on UniProtKB P63973.
ProteinModelPortal	Q8NR20.
SMR	Q8NR20. Positions 7-318.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019226. 3344018.
GenomeReviews	Gene locus Cgl1244 in contig BA000036_GR. Gene locus cg1401 in contig BX927147_GR.
KEGG	cgb:cg1401. cgl:NCgl1196.
PATRIC	21494530. VBICorGlu203724_1221.
Phylogenomic databases
HOGENOM	HBG620317.
OMA	ENVRTIR.
PhylomeDB	Q8NR20.
ProtClustDB	PRK07956.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1401-MONOMER.
Family and domain databases
HAMAP	MF_01588. DNA_ligase_A. [Tree]
InterPro	IPR001357. BRCT. IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR010994. RuvA_2-like. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01972.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
SMART	SM00292. BRCT. 1 hit. SM00532. LIGANc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF47781. RuvA_2_like. 1 hit.
TIGRFAMs	TIGR00575. Dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_CORGL

Accession

Primary (citable) accession number: Q8NR20
Secondary accession number(s): Q6M5U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program