Reviewed, UniProtKB/Swiss-Prot Q8NR14 (K6PF_CORGL)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	Cgl1250, cg1409

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	6-phosphofructokinase HAMAP MF_00339		PRO_0000111948
Regions
	Nucleotide binding	23 – 27	5	ATP By similarity
	Nucleotide binding	158 – 162	5	ATP By similarity
	Nucleotide binding	175 – 191	17	ATP By similarity
Sites
	Active site	131	1	Proton acceptor By similarity
	Metal binding	189	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	270	1	Substrate By similarity
	Binding site	276	1	Substrate By similarity
	Binding site	279	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NR14-1 [UniParc]. Last modified October 19, 2002. Version 1. Checksum: 96713869752962B0	FASTA	346	37,464
	10 20 30 40 50 60 MEDMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLGD RRVQLYDDED 70 80 90 100 110 120 IDRILLRGGT ILGTGRLHPD KFKAGIDQIK ANLEDAGIDA LIPIGGEGTL KGAKWLSDNG 130 140 150 160 170 180 IPVVGVPKTI DNDVNGTDFT FGFDTAVAVA TDAVDRLHTT AESHNRVMIV EVMGRHVGWI 190 200 210 220 230 240 ALHAGMAGGA HYTVIPEVPF DIAEICKAME RRFQMGEKYG IIVVAEGALP REGTMELREG 250 260 270 280 290 300 HIDQFGHKTF TGIGQQIADE IHVRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR 310 320 330 340 ACHEGSFDKV VALKGESIEM ITFEEAVGTL KEVPFERWVT AQAMFG

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000036 Genomic DNA. Translation: BAB98643.1. BX927151 Genomic DNA. Translation: CAF19953.1. Different initiation.
RefSeq	NP_600473.1. YP_225539.1.
3D structure databases
HSSP	HSSP built from PDB template 3PFK based on UniProtKB P00512.
ModBase	Search...
2-D gel databases
World-2DPAGE	0001:Q8NR14.
Proteomic databases
PRIDE	Q8NR14.
Genome annotation databases
GeneID	1019232. 3344017.
GenomeReviews	Gene locus Cgl1250 in contig BA000036_GR. Gene locus cg1409 in contig BX927147_GR.
KEGG	cgb:cg1409. cgl:NCgl1202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NR14.
OMA	Q8NR14. ESHHRIM.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG1409-MON.
BRENDA	2.7.1.11. 812.
Family and domain databases
HAMAP	MF_00339. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012829. PFK. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02483. PFK_mixed. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_CORGL

Accession

Primary (citable) accession number: Q8NR14

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)