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Q8NR14 (K6PF_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructokinase

Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:Cgl1250, cg1409
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase family.

Sequence caution

The sequence CAF19953.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3463466-phosphofructokinase HAMAP-Rule MF_00339
PRO_0000111948

Regions

Nucleotide binding23 – 275ATP By similarity
Nucleotide binding158 – 1625ATP By similarity
Nucleotide binding175 – 19117ATP By similarity

Sites

Active site1311Proton acceptor By similarity
Metal binding1891Magnesium; via carbonyl oxygen By similarity
Binding site1661Substrate By similarity
Binding site2701Substrate By similarity
Binding site2761Substrate By similarity
Binding site2791Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8NR14 [UniParc].

Last modified October 19, 2002. Version 1.
Checksum: 96713869752962B0

FASTA34637,464
        10         20         30         40         50         60 
MEDMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLGD RRVQLYDDED 

        70         80         90        100        110        120 
IDRILLRGGT ILGTGRLHPD KFKAGIDQIK ANLEDAGIDA LIPIGGEGTL KGAKWLSDNG 

       130        140        150        160        170        180 
IPVVGVPKTI DNDVNGTDFT FGFDTAVAVA TDAVDRLHTT AESHNRVMIV EVMGRHVGWI 

       190        200        210        220        230        240 
ALHAGMAGGA HYTVIPEVPF DIAEICKAME RRFQMGEKYG IIVVAEGALP REGTMELREG 

       250        260        270        280        290        300 
HIDQFGHKTF TGIGQQIADE IHVRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR 

       310        320        330        340 
ACHEGSFDKV VALKGESIEM ITFEEAVGTL KEVPFERWVT AQAMFG 

« Hide

References

[1]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000036 Genomic DNA. Translation: BAB98643.1.
BX927151 Genomic DNA. Translation: CAF19953.1. Different initiation.
RefSeqNP_600473.1. NC_003450.3.
YP_225539.1. NC_006958.1.

3D structure databases

ProteinModelPortalQ8NR14.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg1409.

2D gel databases

World-2DPAGE0001:Q8NR14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB98643; BAB98643; BAB98643.
CAF19953; CAF19953; cg1409.
GeneID1019232.
3344017.
KEGGcgb:cg1409.
cgl:NCgl1202.
PATRIC21494542. VBICorGlu203724_1227.

Phylogenomic databases

eggNOGCOG0205.
KOK00850.
OMAARACHEG.
OrthoDBEOG644ZRM.
ProtClustDBPRK03202.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02483. PFK_mixed. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK6PF_CORGL
AccessionPrimary (citable) accession number: Q8NR14
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways