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Q8NR14

- PFKA_CORGL

UniProt

Q8NR14 - PFKA_CORGL

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, Cgl1250, cg1409
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131ATP; via amide nitrogen By similarity
Metal bindingi107 – 1071Magnesium; catalytic By similarity
Sitei108 – 1081Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei131 – 1311Proton acceptor By similarity
Binding sitei166 – 1661Substrate; shared with dimeric partner By similarity
Binding sitei226 – 2261Substrate By similarity
Binding sitei270 – 2701Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATP By similarity
Nucleotide bindingi106 – 1094ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:Cgl1250, cg1409
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_0000111948Add
BLAST

2D gel databases

World-2DPAGE0001:Q8NR14.

Interactioni

Subunit structurei

Homodimer or homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg1409.

Structurei

3D structure databases

ProteinModelPortaliQ8NR14.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1313Substrate binding By similarity
Regioni173 – 1753Substrate binding By similarity
Regioni276 – 2794Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
KOiK00850.
OMAiMELREGH.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NR14-1 [UniParc]FASTAAdd to Basket

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MEDMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLGD    50
RRVQLYDDED IDRILLRGGT ILGTGRLHPD KFKAGIDQIK ANLEDAGIDA 100
LIPIGGEGTL KGAKWLSDNG IPVVGVPKTI DNDVNGTDFT FGFDTAVAVA 150
TDAVDRLHTT AESHNRVMIV EVMGRHVGWI ALHAGMAGGA HYTVIPEVPF 200
DIAEICKAME RRFQMGEKYG IIVVAEGALP REGTMELREG HIDQFGHKTF 250
TGIGQQIADE IHVRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR 300
ACHEGSFDKV VALKGESIEM ITFEEAVGTL KEVPFERWVT AQAMFG 346
Length:346
Mass (Da):37,464
Last modified:October 19, 2002 - v1
Checksum:i96713869752962B0
GO

Sequence cautioni

The sequence CAF19953.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB98643.1.
BX927151 Genomic DNA. Translation: CAF19953.1. Different initiation.
RefSeqiNP_600473.1. NC_003450.3.
WP_011265707.1. NC_006958.1.
YP_225539.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98643; BAB98643; BAB98643.
CAF19953; CAF19953; cg1409.
GeneIDi1019232.
KEGGicgb:cg1409.
cgl:NCgl1202.
PATRICi21494542. VBICorGlu203724_1227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB98643.1 .
BX927151 Genomic DNA. Translation: CAF19953.1 . Different initiation.
RefSeqi NP_600473.1. NC_003450.3.
WP_011265707.1. NC_006958.1.
YP_225539.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q8NR14.
ModBasei Search...

Protein-protein interaction databases

STRINGi 196627.cg1409.

2D gel databases

World-2DPAGE 0001:Q8NR14.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB98643 ; BAB98643 ; BAB98643 .
CAF19953 ; CAF19953 ; cg1409 .
GeneIDi 1019232.
KEGGi cgb:cg1409.
cgl:NCgl1202.
PATRICi 21494542. VBICorGlu203724_1227.

Phylogenomic databases

eggNOGi COG0205.
KOi K00850.
OMAi MELREGH.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiPFKA_CORGL
AccessioniPrimary (citable) accession number: Q8NR14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: September 3, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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