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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131ATP; via amide nitrogenUniRule annotation
Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
Sitei108 – 1081Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei131 – 1311Proton acceptorUniRule annotation
Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
Binding sitei226 – 2261SubstrateUniRule annotation
Binding sitei270 – 2701Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATPUniRule annotation
Nucleotide bindingi106 – 1094ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Cgl1250, cg1409
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001009 Componenti: Chromosome UP000000582 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346ATP-dependent 6-phosphofructokinasePRO_0000111948Add
BLAST

2D gel databases

World-2DPAGE0001:Q8NR14.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg1409.

Structurei

3D structure databases

ProteinModelPortaliQ8NR14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1313Substrate bindingUniRule annotation
Regioni173 – 1753Substrate bindingUniRule annotation
Regioni276 – 2794Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
KOiK00850.
OMAiTVPRERY.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NR14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDMRIATLT SGGDCPGLNA VIRGIVRTAS NEFGSTVVGY QDGWEGLLGD
60 70 80 90 100
RRVQLYDDED IDRILLRGGT ILGTGRLHPD KFKAGIDQIK ANLEDAGIDA
110 120 130 140 150
LIPIGGEGTL KGAKWLSDNG IPVVGVPKTI DNDVNGTDFT FGFDTAVAVA
160 170 180 190 200
TDAVDRLHTT AESHNRVMIV EVMGRHVGWI ALHAGMAGGA HYTVIPEVPF
210 220 230 240 250
DIAEICKAME RRFQMGEKYG IIVVAEGALP REGTMELREG HIDQFGHKTF
260 270 280 290 300
TGIGQQIADE IHVRLGHDVR TTVLGHIQRG GTPTAFDRVL ATRYGVRAAR
310 320 330 340
ACHEGSFDKV VALKGESIEM ITFEEAVGTL KEVPFERWVT AQAMFG
Length:346
Mass (Da):37,464
Last modified:October 19, 2002 - v1
Checksum:i96713869752962B0
GO

Sequence cautioni

The sequence CAF19953.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98643.1.
BX927151 Genomic DNA. Translation: CAF19953.1. Different initiation.
RefSeqiNP_600473.1. NC_003450.3.
YP_225539.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98643; BAB98643; BAB98643.
GeneIDi1019232.
KEGGicgb:cg1409.
cgl:NCgl1202.
PATRICi21494542. VBICorGlu203724_1227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98643.1.
BX927151 Genomic DNA. Translation: CAF19953.1. Different initiation.
RefSeqiNP_600473.1. NC_003450.3.
YP_225539.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NR14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg1409.

2D gel databases

World-2DPAGE0001:Q8NR14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98643; BAB98643; BAB98643.
GeneIDi1019232.
KEGGicgb:cg1409.
cgl:NCgl1202.
PATRICi21494542. VBICorGlu203724_1227.

Phylogenomic databases

eggNOGiCOG0205.
KOiK00850.
OMAiTVPRERY.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiPFKA_CORGL
AccessioniPrimary (citable) accession number: Q8NR14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: April 1, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.