Q8NQZ9 (ILVD_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Cgl1268, cg1432

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103463
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	78	1	Missing in CAB57218. Ref.1
	Sequence conflict	157 – 158	2	DG → ER in CAB57218. Ref.1
	Sequence conflict	188	1	A → R in CAB57218. Ref.1
	Sequence conflict	332	1	G → R in CAB57218. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8NQZ9 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B422D52E6ACDB250	FASTA	613	64,674
	10 20 30 40 50 60 MIPLRSKVTT VGRNAAGARA LWRATGTKEN EFGKPIVAIV NSYTQFVPGH VHLKNVGDIV 70 80 90 100 110 120 ADAVRKAGGV PKEFNTIAVD DGIAMGHGGM LYSLPSREII ADSVEYMVNA HTADAMVCIS 130 140 150 160 170 180 NCDKITPGML NAAMRLNIPV VFVSGGPMEA GKAVVVDGVA HAPTDLITAI SASASDAVDD 190 200 210 220 230 240 AGLAAVEASA CPTCGSCSGM FTANSMNCLT EALGLSLPGN GSTLATHAAR RALFEKAGET 250 260 270 280 290 300 VVELCRRYYG EEDESVLPRG IATKKAFENA MALDMAMGGS TNTILHILAA AQEGEVDFDL 310 320 330 340 350 360 ADIDELSKNV PCLSKVAPNS DYHMEDVHRA GGIPALLGEL NRGGLLNKDV HSVHSNDLEG 370 380 390 400 410 420 WLDDWDIRSG KTTEVATELF HAAPGGIRTT EAFSTENRWD ELDTDAAKGC IRDVEHAYTA 430 440 450 460 470 480 DGGLVVLRGN ISPDGAVIKS AGIEEELWNF TGPARVVESQ EEAVSVILTK TIQAGEVLVV 490 500 510 520 530 540 RYEGPSGGPG MQEMLHPTAF LKGSGLGKKC ALITDGRFSG GSSGLSIGHV SPEAAHGGVI 550 560 570 580 590 600 GLIENGDIVS IDVHNRKLEV QVSDEELQRR RDAMNASEKP WQPVNRNRVV TKALRAYAKM 610 ATSADKGAVR QVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and molecular analysis of the ilvD gene of Corynebacterium glutamicum." Ondrejkova A., Eggeling L., Sahm H. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ012293 Genomic DNA. Translation: CAB57218.1. BA000036 Genomic DNA. Translation: BAB98661.1. BX927151 Genomic DNA. Translation: CAF19971.1.
RefSeq	NP_600490.1. NC_003450.3. YP_225557.1. NC_006958.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019249. 3344114.
GenomeReviews	Gene locus Cgl1268 in contig BA000036_GR. Gene locus cg1432 in contig BX927147_GR.
KEGG	cgb:cg1432. cgl:NCgl1219.
PATRIC	21494578. VBICorGlu203724_1245.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q8NQZ9.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1432-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CORGL

Accession

Primary (citable) accession number: Q8NQZ9
Secondary accession number(s): Q9RM73

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program