Q8NQK6 (DER_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase Der

Alternative name(s):
GTP-binding protein EngA

Gene names

Name:	der
Synonyms:	engA
Ordered Locus Names:	Cgl1428, cg1617

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	GTPase that plays an essential role in the late steps of ribosome biogenesis By similarity. HAMAP MF_00195
Subunit structure	Associates with the 50S ribosomal subunit By similarity.
Sequence similarities	Belongs to the Era/MnmE GTP-binding protein family. Der subfamily. Contains 2 G (guanine nucleotide-binding) domains. Contains 1 KH-like domain.
Sequence caution	The sequence CAF21438.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Domain	Repeat
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	GTPase Der HAMAP MF_00195		PRO_0000178988
Regions
	Domain	92 – 197	106	G 1
	Domain	265 – 373	109	G 2
	Domain	427 – 510	84	KH-like
	Nucleotide binding	87 – 94	8	GTP 1 Potential
	Nucleotide binding	134 – 138	5	GTP 1 Potential
	Nucleotide binding	196 – 199	4	GTP 1 Potential
	Nucleotide binding	260 – 267	8	GTP 2 Potential
	Nucleotide binding	307 – 311	5	GTP 2 Potential
	Nucleotide binding	372 – 375	4	GTP 2 Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8NQK6 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: BC3EDF3C0C503083	FASTA	519	57,666
	10 20 30 40 50 60 MDVEGAFADE EELAPHGGWA SADFDPAEFG YEDSDDDFDA EDFDETEFSN PDFGEDYSDE 70 80 90 100 110 120 DWEEIETAFG FDPSHLEEAL CTVAIVGRPN VGKSTLVNRF IGRREAVVED FPGVTRDRIS 130 140 150 160 170 180 YISDWGGHRF WVQDTGGWDP NVKGIHASIA QQAEVAMSTA DVIVFVVDTK VGITETDSVM 190 200 210 220 230 240 AAKLLRSEVP VILVANKFDS DSQWADMAEF YSLGLGDPYP VSAQHGRGGA DVLDKVLELF 250 260 270 280 290 300 PEEPRSKSIV EGPRRVALVG KPNVGKSSLL NKFAGETRSV VDNVAGTTVD PVDSLIQLDQ 310 320 330 340 350 360 KLWKFVDTAG LRKKVKTASG HEYYASLRTH GAIDAAELCV LLIDSSEPIT EQDQRVLAMI 370 380 390 400 410 420 TDAGKALVIA FNKWDLMDED RRIDLDRELD LQLAHVPWAK RINISAKTGR ALQRLEPAML 430 440 450 460 470 480 EALDNWDRRI STGQLNTWLR EAIAANPPPM RGGRLPRVLF ATQASTQPPV IVLFTTGFLE 490 500 510 AGYRRYLERK FRERFGFEGT PVRIAVRVRE RRGKGGNKQ

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB98821.1. BX927152 Genomic DNA. Translation: CAF21438.1. Different initiation.
RefSeq	NP_600646.1. NC_003450.3. YP_225714.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NQK6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019403. 3344088.
GenomeReviews	Gene locus Cgl1428 in contig BA000036_GR. Gene locus cg1617 in contig BX927147_GR.
KEGG	cgb:cg1617. cgl:NCgl1373.
PATRIC	21494899. VBICorGlu203724_1396.
Phylogenomic databases
HOGENOM	HBG592135.
OMA	QGADFYA.
PhylomeDB	Q8NQK6.
ProtClustDB	PRK03003.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1617-MONOMER.
Family and domain databases
HAMAP	MF_00195. GTPase_Der. [Tree]
InterPro	IPR016484. GTP-bd_EngA. IPR006073. GTP_binding_domain. IPR015946. KH_dom-like_a/b. IPR005225. Small_GTP-bd_dom. [Graphical view]
Gene3D	G3DSA:3.30.300.20. KH_prok. 1 hit.
KO	K03977.
PANTHER	PTHR11649:SF5. PTHR11649:SF5. 1 hit.
Pfam	PF01926. MMR_HSR1. 2 hits. [Graphical view]
PIRSF	PIRSF006485. GTP-binding_EngA. 1 hit.
PRINTS	PR00326. GTP1OBG.
TIGRFAMs	TIGR03594. GTPase_EngA. 1 hit. TIGR00231. Small_GTP. 2 hits.
ProtoNet	Search...

Entry information

Entry name

DER_CORGL

Accession

Primary (citable) accession number: Q8NQK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program