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Protein

Thiamine biosynthesis multifunctional protein ThiED

Gene

theD

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).By similarity
Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.By similarity

Catalytic activityi

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathway:ithiamine diphosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Thiamine biosynthesis multifunctional protein ThiED (theD)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Pathway:ithiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine biosynthesis multifunctional protein ThiED (theD), Thiamine-phosphate synthase (thiE)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691HMP-PPBy similarity
Metal bindingi70 – 701MagnesiumBy similarity
Metal bindingi88 – 881MagnesiumBy similarity
Binding sitei107 – 1071HMP-PPBy similarity
Binding sitei143 – 1431HMP-PPBy similarity
Binding sitei174 – 1741THZ-P; via amide nitrogenBy similarity
Binding sitei282 – 2821HydroxymethylpyrimidineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-1450-MONOMER.
UniPathwayiUPA00060; UER00138.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthesis multifunctional protein ThiED
Including the following 2 domains:
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TMP-PPase
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:theD
Synonyms:thiD1
Ordered Locus Names:Cgl1463, cg1654
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 763763Thiamine biosynthesis multifunctional protein ThiEDPRO_0000192039Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196627.cg1654.

Structurei

3D structure databases

ProteinModelPortaliQ8NQH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 210210Thiamine-phosphate synthaseAdd
BLAST
Regioni37 – 415HMP-PP bindingBy similarity
Regioni140 – 1423THZ-P bindingBy similarity
Regioni194 – 1952THZ-P bindingBy similarity
Regioni245 – 500256Hydroxymethylpyrimidine/phosphomethylpyrimidine kinaseAdd
BLAST
Regioni550 – 763214Thiaminase-2Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the thiamine-phosphate synthase family.Curated
In the central section; belongs to the ThiD family.Curated
In the C-terminal section; belongs to the thiaminase-2 family.Curated

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000225275.
KOiK14153.
OMAiACTAYTR.
OrthoDBiEOG6XWV53.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR016084. Haem_Oase-like_multi-hlx.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF48613. SSF48613. 1 hit.
SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NQH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDFSLYLVT DPVLGGGPEK VAGIVDSAIS GGVSVVQLRD KNSGVEDVRA
60 70 80 90 100
AAKELKELCD ARGVALVVND YLDIAVELGL HLHIGQGDTP YTQARELLPA
110 120 130 140 150
HLELGLSIEN LDQLHAVIAQ CAETGVALPD VIGIGPVAST ATKPDAAPAL
160 170 180 190 200
GVEGIAEIAA VAQDHGIASV AIGGVGLRNA AELAATPIDG LCVVSEIMTA
210 220 230 240 250
ANPAAAATRL RTAFQPTFSP ETQTELSQTE LQGAFVNSPS APRVLSIAGT
260 270 280 290 300
DPTGGAGIQA DLKSIAAGGG YGMCVVTSLV AQNTHGVNTI HTPPLTFLEE
310 320 330 340 350
QLEAVFSDVT VDAIKLGMLG SADTVDLVAS WLGSHEHGPV VLDPVMIATS
360 370 380 390 400
GDRLLDASAE ESLRRLAVHV DVVTPNIPEL AVLCDSAPAI TMDEAIAQAQ
410 420 430 440 450
GFARTHDTIV IVKGGHLTGA LADNAVVRPD GSVFQVENLR VNTTNSHGTG
460 470 480 490 500
CSLSASLATK IAAGESVEKA LEWSTRWLNE ALRHADHLAV GTGNGPVDHG
510 520 530 540 550
HLARRMTHAA ETTPWAHLRA PRLDGATAAS FTTPSTVKSP APRIEPAGPF
560 570 580 590 600
TRALWEASGD IIAGINSSDF ITMLGDGTLR RPEFDFYIDQ DAQYLAQYSR
610 620 630 640 650
ALARLSSIAP DSHAQIEWAQ SAAECLVVEA ELHRSYMAGK EVSAPSHITM
660 670 680 690 700
AYTDFLIART YTEDYVCGVA AVLPCYWLYA EIGLMLAEQN HDEHPYKDWL
710 720 730 740 750
NTYSGEEFIA GTRAAIARLE KALENAGAEQ RVDAARAFLS ASVHEREFFD
760
QATRHGWTMV GSS
Length:763
Mass (Da):80,355
Last modified:May 24, 2004 - v2
Checksum:iCA992321E884577F
GO

Sequence cautioni

The sequence BAB98856.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98856.1. Different initiation.
BX927152 Genomic DNA. Translation: CAF21472.1.
RefSeqiNP_600680.3. NC_003450.3.
WP_011014382.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98856; BAB98856; BAB98856.
CAF21472; CAF21472; cg1654.
GeneIDi1019437.
KEGGicgb:cg1654.
cgl:NCgl1407.
PATRICi21494969. VBICorGlu203724_1430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98856.1. Different initiation.
BX927152 Genomic DNA. Translation: CAF21472.1.
RefSeqiNP_600680.3. NC_003450.3.
WP_011014382.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NQH1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg1654.

Protocols and materials databases

DNASUi3345397.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98856; BAB98856; BAB98856.
CAF21472; CAF21472; cg1654.
GeneIDi1019437.
KEGGicgb:cg1654.
cgl:NCgl1407.
PATRICi21494969. VBICorGlu203724_1430.

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000225275.
KOiK14153.
OMAiACTAYTR.
OrthoDBiEOG6XWV53.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00138.
UPA00060; UER00141.
BioCyciCGLU196627:GJDM-1450-MONOMER.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR016084. Haem_Oase-like_multi-hlx.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF48613. SSF48613. 1 hit.
SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiTHIED_CORGL
AccessioniPrimary (citable) accession number: Q8NQH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: July 22, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.