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Q8NQH1

- THIED_CORGL

UniProt

Q8NQH1 - THIED_CORGL

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Protein

Thiamine biosynthesis multifunctional protein ThiED

Gene
theD, thiD1, Cgl1463, cg1654
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) By similarity.UniRule annotation
Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P By similarity.UniRule annotation

Catalytic activityi

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.UniRule annotation
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.UniRule annotation
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691HMP-PP By similarity
Metal bindingi70 – 701Magnesium By similarity
Metal bindingi88 – 881Magnesium By similarity
Binding sitei107 – 1071HMP-PP By similarity
Binding sitei143 – 1431HMP-PP By similarity
Binding sitei174 – 1741THZ-P; via amide nitrogen By similarity
Binding sitei282 – 2821Hydroxymethylpyrimidine By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxymethylpyrimidine kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. phosphomethylpyrimidine kinase activity Source: UniProtKB-EC
  5. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-1450-MONOMER.
UniPathwayiUPA00060; UER00138.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthesis multifunctional protein ThiED
Including the following 2 domains:
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TMP-PPase
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:theD
Synonyms:thiD1
Ordered Locus Names:Cgl1463, cg1654
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 763763Thiamine biosynthesis multifunctional protein ThiEDUniRule annotationPRO_0000192039Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196627.cg1654.

Structurei

3D structure databases

ProteinModelPortaliQ8NQH1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 210210Thiamine-phosphate synthaseUniRule annotationAdd
BLAST
Regioni37 – 415HMP-PP binding By similarity
Regioni140 – 1423THZ-P binding By similarity
Regioni194 – 1952THZ-P binding By similarity
Regioni245 – 500256Hydroxymethylpyrimidine/phosphomethylpyrimidine kinaseUniRule annotationAdd
BLAST
Regioni550 – 763214Thiaminase-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the thiamine-phosphate synthase family.
In the central section; belongs to the ThiD family.
In the C-terminal section; belongs to the thiaminase-2 family.

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000225275.
KOiK14153.
OMAiGFWEMFP.
OrthoDBiEOG6XWV53.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR016084. Haem_Oase-like_multi-hlx.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF48613. SSF48613. 1 hit.
SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NQH1-1 [UniParc]FASTAAdd to Basket

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MTDFSLYLVT DPVLGGGPEK VAGIVDSAIS GGVSVVQLRD KNSGVEDVRA    50
AAKELKELCD ARGVALVVND YLDIAVELGL HLHIGQGDTP YTQARELLPA 100
HLELGLSIEN LDQLHAVIAQ CAETGVALPD VIGIGPVAST ATKPDAAPAL 150
GVEGIAEIAA VAQDHGIASV AIGGVGLRNA AELAATPIDG LCVVSEIMTA 200
ANPAAAATRL RTAFQPTFSP ETQTELSQTE LQGAFVNSPS APRVLSIAGT 250
DPTGGAGIQA DLKSIAAGGG YGMCVVTSLV AQNTHGVNTI HTPPLTFLEE 300
QLEAVFSDVT VDAIKLGMLG SADTVDLVAS WLGSHEHGPV VLDPVMIATS 350
GDRLLDASAE ESLRRLAVHV DVVTPNIPEL AVLCDSAPAI TMDEAIAQAQ 400
GFARTHDTIV IVKGGHLTGA LADNAVVRPD GSVFQVENLR VNTTNSHGTG 450
CSLSASLATK IAAGESVEKA LEWSTRWLNE ALRHADHLAV GTGNGPVDHG 500
HLARRMTHAA ETTPWAHLRA PRLDGATAAS FTTPSTVKSP APRIEPAGPF 550
TRALWEASGD IIAGINSSDF ITMLGDGTLR RPEFDFYIDQ DAQYLAQYSR 600
ALARLSSIAP DSHAQIEWAQ SAAECLVVEA ELHRSYMAGK EVSAPSHITM 650
AYTDFLIART YTEDYVCGVA AVLPCYWLYA EIGLMLAEQN HDEHPYKDWL 700
NTYSGEEFIA GTRAAIARLE KALENAGAEQ RVDAARAFLS ASVHEREFFD 750
QATRHGWTMV GSS 763
Length:763
Mass (Da):80,355
Last modified:May 24, 2004 - v2
Checksum:iCA992321E884577F
GO

Sequence cautioni

The sequence BAB98856.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB98856.1. Different initiation.
BX927152 Genomic DNA. Translation: CAF21472.1.
RefSeqiNP_600680.3. NC_003450.3.
WP_011014382.1. NC_006958.1.
YP_225748.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98856; BAB98856; BAB98856.
CAF21472; CAF21472; cg1654.
GeneIDi1019437.
KEGGicgb:cg1654.
cgl:NCgl1407.
PATRICi21494969. VBICorGlu203724_1430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB98856.1 . Different initiation.
BX927152 Genomic DNA. Translation: CAF21472.1 .
RefSeqi NP_600680.3. NC_003450.3.
WP_011014382.1. NC_006958.1.
YP_225748.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q8NQH1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg1654.

Protocols and materials databases

DNASUi 3345397.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB98856 ; BAB98856 ; BAB98856 .
CAF21472 ; CAF21472 ; cg1654 .
GeneIDi 1019437.
KEGGi cgb:cg1654.
cgl:NCgl1407.
PATRICi 21494969. VBICorGlu203724_1430.

Phylogenomic databases

eggNOGi COG0351.
HOGENOMi HOG000225275.
KOi K14153.
OMAi GFWEMFP.
OrthoDBi EOG6XWV53.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00138 .
UPA00060 ; UER00141 .
BioCyci CGLU196627:GJDM-1450-MONOMER.

Family and domain databases

Gene3Di 1.20.910.10. 1 hit.
3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPi MF_00097. TMP_synthase.
InterProi IPR013785. Aldolase_TIM.
IPR016084. Haem_Oase-like_multi-hlx.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view ]
Pfami PF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view ]
SUPFAMi SSF48613. SSF48613. 1 hit.
SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiTHIED_CORGL
AccessioniPrimary (citable) accession number: Q8NQH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: September 3, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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