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Q8NQH1

- THIED_CORGL

UniProt

Q8NQH1 - THIED_CORGL

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Protein

Thiamine biosynthesis multifunctional protein ThiED

Gene

theD

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).By similarity
Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.By similarity

Catalytic activityi

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

Cofactori

Binds 1 magnesium ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691HMP-PPBy similarity
Metal bindingi70 – 701MagnesiumBy similarity
Metal bindingi88 – 881MagnesiumBy similarity
Binding sitei107 – 1071HMP-PPBy similarity
Binding sitei143 – 1431HMP-PPBy similarity
Binding sitei174 – 1741THZ-P; via amide nitrogenBy similarity
Binding sitei282 – 2821HydroxymethylpyrimidineBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxymethylpyrimidine kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. phosphomethylpyrimidine kinase activity Source: UniProtKB-EC
  5. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-1450-MONOMER.
UniPathwayiUPA00060; UER00138.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthesis multifunctional protein ThiED
Including the following 2 domains:
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TMP-PPase
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:theD
Synonyms:thiD1
Ordered Locus Names:Cgl1463, cg1654
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 763763Thiamine biosynthesis multifunctional protein ThiEDPRO_0000192039Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196627.cg1654.

Structurei

3D structure databases

ProteinModelPortaliQ8NQH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 210210Thiamine-phosphate synthaseAdd
BLAST
Regioni37 – 415HMP-PP bindingBy similarity
Regioni140 – 1423THZ-P bindingBy similarity
Regioni194 – 1952THZ-P bindingBy similarity
Regioni245 – 500256Hydroxymethylpyrimidine/phosphomethylpyrimidine kinaseAdd
BLAST
Regioni550 – 763214Thiaminase-2Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the thiamine-phosphate synthase family.Curated
In the central section; belongs to the ThiD family.Curated
In the C-terminal section; belongs to the thiaminase-2 family.Curated

Phylogenomic databases

eggNOGiCOG0351.
HOGENOMiHOG000225275.
KOiK14153.
OMAiGFWEMFP.
OrthoDBiEOG6XWV53.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR016084. Haem_Oase-like_multi-hlx.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF48613. SSF48613. 1 hit.
SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NQH1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDFSLYLVT DPVLGGGPEK VAGIVDSAIS GGVSVVQLRD KNSGVEDVRA
60 70 80 90 100
AAKELKELCD ARGVALVVND YLDIAVELGL HLHIGQGDTP YTQARELLPA
110 120 130 140 150
HLELGLSIEN LDQLHAVIAQ CAETGVALPD VIGIGPVAST ATKPDAAPAL
160 170 180 190 200
GVEGIAEIAA VAQDHGIASV AIGGVGLRNA AELAATPIDG LCVVSEIMTA
210 220 230 240 250
ANPAAAATRL RTAFQPTFSP ETQTELSQTE LQGAFVNSPS APRVLSIAGT
260 270 280 290 300
DPTGGAGIQA DLKSIAAGGG YGMCVVTSLV AQNTHGVNTI HTPPLTFLEE
310 320 330 340 350
QLEAVFSDVT VDAIKLGMLG SADTVDLVAS WLGSHEHGPV VLDPVMIATS
360 370 380 390 400
GDRLLDASAE ESLRRLAVHV DVVTPNIPEL AVLCDSAPAI TMDEAIAQAQ
410 420 430 440 450
GFARTHDTIV IVKGGHLTGA LADNAVVRPD GSVFQVENLR VNTTNSHGTG
460 470 480 490 500
CSLSASLATK IAAGESVEKA LEWSTRWLNE ALRHADHLAV GTGNGPVDHG
510 520 530 540 550
HLARRMTHAA ETTPWAHLRA PRLDGATAAS FTTPSTVKSP APRIEPAGPF
560 570 580 590 600
TRALWEASGD IIAGINSSDF ITMLGDGTLR RPEFDFYIDQ DAQYLAQYSR
610 620 630 640 650
ALARLSSIAP DSHAQIEWAQ SAAECLVVEA ELHRSYMAGK EVSAPSHITM
660 670 680 690 700
AYTDFLIART YTEDYVCGVA AVLPCYWLYA EIGLMLAEQN HDEHPYKDWL
710 720 730 740 750
NTYSGEEFIA GTRAAIARLE KALENAGAEQ RVDAARAFLS ASVHEREFFD
760
QATRHGWTMV GSS
Length:763
Mass (Da):80,355
Last modified:May 24, 2004 - v2
Checksum:iCA992321E884577F
GO

Sequence cautioni

The sequence BAB98856.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB98856.1. Different initiation.
BX927152 Genomic DNA. Translation: CAF21472.1.
RefSeqiNP_600680.3. NC_003450.3.
WP_011014382.1. NC_006958.1.
YP_225748.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98856; BAB98856; BAB98856.
CAF21472; CAF21472; cg1654.
GeneIDi1019437.
3345397.
KEGGicgb:cg1654.
cgl:NCgl1407.
PATRICi21494969. VBICorGlu203724_1430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB98856.1 . Different initiation.
BX927152 Genomic DNA. Translation: CAF21472.1 .
RefSeqi NP_600680.3. NC_003450.3.
WP_011014382.1. NC_006958.1.
YP_225748.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q8NQH1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg1654.

Protocols and materials databases

DNASUi 3345397.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB98856 ; BAB98856 ; BAB98856 .
CAF21472 ; CAF21472 ; cg1654 .
GeneIDi 1019437.
3345397.
KEGGi cgb:cg1654.
cgl:NCgl1407.
PATRICi 21494969. VBICorGlu203724_1430.

Phylogenomic databases

eggNOGi COG0351.
HOGENOMi HOG000225275.
KOi K14153.
OMAi GFWEMFP.
OrthoDBi EOG6XWV53.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00138 .
UPA00060 ; UER00141 .
BioCyci CGLU196627:GJDM-1450-MONOMER.

Family and domain databases

Gene3Di 1.20.910.10. 1 hit.
3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPi MF_00097. TMP_synthase.
InterProi IPR013785. Aldolase_TIM.
IPR016084. Haem_Oase-like_multi-hlx.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view ]
Pfami PF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view ]
SUPFAMi SSF48613. SSF48613. 1 hit.
SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00097. HMP-P_kinase. 1 hit.
TIGR00693. thiE. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiTHIED_CORGL
AccessioniPrimary (citable) accession number: Q8NQH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: October 29, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3