ID   THIM_CORGL              Reviewed;         268 AA.
AC   Q8NQH0;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 49.
DE   RecName: Full=Hydroxyethylthiazole kinase;
DE            EC=2.7.1.50;
DE   AltName: Full=4-methyl-5-beta-hydroxyethylthiazole kinase;
DE            Short=Thz kinase;
DE            Short=TH kinase;
GN   Name=thiM; OrderedLocusNames=Cgl1464, cg1655;
OS   Corynebacterium glutamicum (Brevibacterium flavum).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Corynebacteriaceae; Corynebacterium.
OX   NCBI_TaxID=1718;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
RA   Nakagawa S.;
RT   "Complete genomic sequence of Corynebacterium glutamicum ATCC 13032.";
RL   Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
RX   MEDLINE=22830012; PubMed=12948626; DOI=10.1016/S0168-1656(03)00154-8;
RA   Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M.,
RA   Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L.,
RA   Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B.,
RA   McHardy A.C., Meyer F., Moeckel B., Pfefferle W., Puehler A.,
RA   Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I.,
RA   Tauch A.;
RT   "The complete Corynebacterium glutamicum ATCC 13032 genome sequence
RT   and its impact on the production of L-aspartate-derived amino acids
RT   and vitamins.";
RL   J. Biotechnol. 104:5-25(2003).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole =
CC       ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.
CC   -!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine pyrophosphate
CC       biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-
CC       5-(2-hydroxyethyl)-thiazole: step 1/1.
CC   -!- SIMILARITY: Belongs to the Thz kinase family.
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DR   EMBL; BA000036; BAB98857.1; -; Genomic_DNA.
DR   EMBL; BX927152; CAF21473.1; -; Genomic_DNA.
DR   RefSeq; NP_600681.1; -.
DR   RefSeq; YP_225749.1; -.
DR   HSSP; P39593; 1EKQ.
DR   GeneID; 1019438; -.
DR   GeneID; 3342857; -.
DR   GenomeReviews; BA000036_GR; Cgl1464.
DR   GenomeReviews; BX927147_GR; cg1655.
DR   KEGG; cgb:cg1655; -.
DR   KEGG; cgl:NCgl1408; -.
DR   HOGENOM; Q8NQH0; -.
DR   OMA; Q8NQH0; ASPVMAH.
DR   BioCyc; CGLU196627-1:CG1655-MON; -.
DR   BRENDA; 2.7.1.50; 812.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004417; F:hydroxyethylthiazole kinase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009228; P:thiamin biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00228; -; 1.
DR   InterPro; IPR000417; Hyethyz_kinase.
DR   InterPro; IPR011144; Hyethyz_kinsmonf.
DR   PANTHER; PTHR20857:SF14; Hyethyz_kinase; 1.
DR   Pfam; PF02110; HK; 1.
DR   PIRSF; PIRSF000513; Thz_kinase; 1.
DR   PRINTS; PR01099; HYETHTZKNASE.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Thiamine biosynthesis; Transferase.
FT   CHAIN         1    268       Hydroxyethylthiazole kinase.
FT                                /FTId=PRO_0000156932.
FT   METAL        93     93       Magnesium 1 (By similarity).
FT   METAL       124    124       Magnesium 1 (By similarity).
FT   BINDING      44     44       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     119    119       ATP (By similarity).
FT   BINDING     165    165       ATP (By similarity).
FT   BINDING     192    192       Substrate; via amide nitrogen (By
FT                                similarity).
SQ   SEQUENCE   268 AA;  26911 MW;  0019CC83C9D954E3 CRC64;
     MANSFLDSLT LVRQNTPLVQ CLTNSVVMQF TANVLLAAGA TPAMVDTPAE SAEFAAVANG
     VLINAGTPSA EQYQGMTKAI EGARKAGTPW VLDPVAVGGL SERTKYAEGI VDKQPAAIRG
     NASEVVALAG LGAGGRGVDA TDSVEVALEA AQLLAKRTGG VVAVSGAEDL IVSADRVTWL
     RSGDPMLQLV IGTGCSLGAL TAAYLGATVD SDISAHDAVL AAHAHVGAAG QIAAQKASAP
     GSFAVAFIDA LYDVDAQAVA SLVDVREA
//