Reviewed, UniProtKB/Swiss-Prot Q8NQH0 (THIM_CORGL)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	Cgl1464, cg1655

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156932
Sites
	Metal binding	93	1	Magnesium 1 By similarity
	Metal binding	124	1	Magnesium 1 By similarity
	Binding site	44	1	Substrate; via amide nitrogen By similarity
	Binding site	119	1	ATP By similarity
	Binding site	165	1	ATP By similarity
	Binding site	192	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NQH0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0019CC83C9D954E3	FASTA	268	26,911
	10 20 30 40 50 60 MANSFLDSLT LVRQNTPLVQ CLTNSVVMQF TANVLLAAGA TPAMVDTPAE SAEFAAVANG 70 80 90 100 110 120 VLINAGTPSA EQYQGMTKAI EGARKAGTPW VLDPVAVGGL SERTKYAEGI VDKQPAAIRG 130 140 150 160 170 180 NASEVVALAG LGAGGRGVDA TDSVEVALEA AQLLAKRTGG VVAVSGAEDL IVSADRVTWL 190 200 210 220 230 240 RSGDPMLQLV IGTGCSLGAL TAAYLGATVD SDISAHDAVL AAHAHVGAAG QIAAQKASAP 250 260 GSFAVAFIDA LYDVDAQAVA SLVDVREA

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000036 Genomic DNA. Translation: BAB98857.1. BX927152 Genomic DNA. Translation: CAF21473.1.
RefSeq	NP_600681.1. YP_225749.1.
3D structure databases
HSSP	HSSP built from PDB template 1EKQ based on UniProtKB P39593.
ModBase	Search...
Genome annotation databases
GeneID	1019438. 3342857.
GenomeReviews	Gene locus Cgl1464 in contig BA000036_GR. Gene locus cg1655 in contig BX927147_GR.
KEGG	cgb:cg1655. cgl:NCgl1408.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NQH0.
OMA	Q8NQH0. ASPVMAH.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG1655-MON.
BRENDA	2.7.1.50. 812.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
ProtoNet	Search...

Entry information

Entry name

THIM_CORGL

Accession

Primary (citable) accession number: Q8NQH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)