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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44Substrate; via amide nitrogenUniRule annotation1
Binding sitei119ATPUniRule annotation1
Binding sitei165ATPUniRule annotation1
Binding sitei192Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCORYNE:G18NG-11047-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:Cgl1464, cg1655
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569321 – 268Hydroxyethylthiazole kinaseAdd BLAST268

Interactioni

Protein-protein interaction databases

STRINGi196627.cg1655.

Structurei

3D structure databases

ProteinModelPortaliQ8NQH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
KOiK00878.
OMAiSPVMAHA.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NQH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSFLDSLT LVRQNTPLVQ CLTNSVVMQF TANVLLAAGA TPAMVDTPAE
60 70 80 90 100
SAEFAAVANG VLINAGTPSA EQYQGMTKAI EGARKAGTPW VLDPVAVGGL
110 120 130 140 150
SERTKYAEGI VDKQPAAIRG NASEVVALAG LGAGGRGVDA TDSVEVALEA
160 170 180 190 200
AQLLAKRTGG VVAVSGAEDL IVSADRVTWL RSGDPMLQLV IGTGCSLGAL
210 220 230 240 250
TAAYLGATVD SDISAHDAVL AAHAHVGAAG QIAAQKASAP GSFAVAFIDA
260
LYDVDAQAVA SLVDVREA
Length:268
Mass (Da):26,911
Last modified:October 1, 2002 - v1
Checksum:i0019CC83C9D954E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98857.1.
BX927152 Genomic DNA. Translation: CAF21473.1.
RefSeqiNP_600681.1. NC_003450.3.
WP_011014383.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98857; BAB98857; BAB98857.
CAF21473; CAF21473; cg1655.
GeneIDi1019438.
KEGGicgb:cg1655.
cgl:NCgl1408.
PATRICi21494971. VBICorGlu203724_1431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98857.1.
BX927152 Genomic DNA. Translation: CAF21473.1.
RefSeqiNP_600681.1. NC_003450.3.
WP_011014383.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NQH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg1655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98857; BAB98857; BAB98857.
CAF21473; CAF21473; cg1655.
GeneIDi1019438.
KEGGicgb:cg1655.
cgl:NCgl1408.
PATRICi21494971. VBICorGlu203724_1431.

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
KOiK00878.
OMAiSPVMAHA.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciCORYNE:G18NG-11047-MONOMER.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIM_CORGL
AccessioniPrimary (citable) accession number: Q8NQH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.