Q8NQ97 (ACNR_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional repressor AcnR

Gene names

Name:	acnR
Ordered Locus Names:	Cgl1541, cg1738

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses the aconitase gene acn. Binds to the imperfect inverted repeat 5'-AGAACGCTTGTACTG-3' in the acn promoter region.
Subunit structure	Homodimer.
Miscellaneous	Deletion of acnR leads to 5-fold increased aconitase activity. Overexpression of acnR leads to a 2-fold decreased aconitase activity.
Sequence similarities	Contains 1 HTH tetR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 188

188

HTH-type transcriptional repressor AcnR

PRO_0000070571

Regions

Domain

10 – 70

61

HTH tetR-type

DNA binding

33 – 52

20

H-T-H motif Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

188

Helix Strand Turn

Details...

Helix

12 – 31

20

Helix

34 – 41

8

Helix

45 – 52

8

Helix

55 – 88

34

Helix

90 – 92

3

Helix

93 – 101

9

Helix

103 – 108

6

Helix

110 – 119

10

Helix

121 – 136

16

Beta strand

142 – 144

3

Helix

146 – 165

20

Helix

173 – 184

12

Sequences

	Sequence		Length	Mass (Da)
	Q8NQ97 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 54F31FA9A184B836	FASTA	188	21,185
	10 20 30 40 50 60 MSVAAGDKPT NSRQEILEGA RRCFAEHGYE GATVRRLEEA TGKSRGAIFH HFGDKENLFL 70 80 90 100 110 120 ALAREDAARM AEVVSENGLV EVMRGMLEDP ERYDWMSVRL EISKQLRTDP VFRAKWIDHQ 130 140 150 160 170 180 SVLDEAVRVR LSRNVDKGQM RTDVPIEVLH TFLETVLDGF ISRLATGAST EGLSEVLDLV EGTVRKRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum." Krug A., Wendisch V.F., Bott M. J. Biol. Chem. 280:585-595(2005) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000036 Genomic DNA. Translation: BAB98934.1.
BX927152 Genomic DNA. Translation: CAF21549.1.

RefSeq

NP_600756.1. NC_003450.3.
YP_225825.1. NC_006958.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AC6	X-ray	2.54	A	2-188	[»]
4ACI	X-ray	1.65	A/B	2-188	[»]
4AF5	X-ray	1.90	A	1-188	[»]

ProteinModelPortal

Q8NQ97.

ModBase

Search...

Protein-protein interaction databases

STRING

196627.cg1738.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAB98934; BAB98934; BAB98934.
CAF21549; CAF21549; cg1738.

GeneID

1019513.
3344470.

KEGG

cgb:cg1738.
cgl:NCgl1483.

PATRIC

21495129. VBICorGlu203724_1508.

Phylogenomic databases

eggNOG

COG1309.

HOGENOM

HOG000248228.

OMA

AIFHHFG.

ProtClustDB

CLSK871936.

Enzyme and pathway databases

BioCyc

CGLU196627:GJDM-1528-MONOMER.

Family and domain databases

Gene3D

1.10.357.10. 1 hit.

InterPro

IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]

Pfam

PF00440. TetR_N. 1 hit.
[Graphical view]

PRINTS

PR00455. HTHTETR.

SUPFAM

SSF46689. Homeodomain_like. 1 hit.
SSF48498. TetR_like_C. 1 hit.

PROSITE

PS01081. HTH_TETR_1. False negative.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ACNR_CORGL

Accession

Primary (citable) accession number: Q8NQ97
Secondary accession number(s): Q6M549

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program