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Q8NP73

- DAPF_CORGL

UniProt

Q8NP73 - DAPF_CORGL

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (25 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan. Involved in the succinylase branch of the diaminopimelate biosynthesis.1 Publication

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151SubstrateBy similarity
    Binding sitei48 – 481SubstrateBy similarity
    Binding sitei74 – 741SubstrateBy similarity
    Active sitei83 – 831Proton donor/acceptorBy similarity
    Binding sitei159 – 1591SubstrateBy similarity
    Sitei161 – 1611Important for catalytic activityBy similarity
    Binding sitei194 – 1941SubstrateBy similarity
    Sitei212 – 2121Important for catalytic activityBy similarity
    Active sitei221 – 2211Proton donor/acceptorBy similarity

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimerase (EC:5.1.1.7)
    Short name:
    DAP epimerase
    Gene namesi
    Name:dapF
    Ordered Locus Names:Cgl1943, cg2129
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 277277Diaminopimelate epimerasePRO_0000149837Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi83 ↔ 221

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi196627.cg2129.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NP73.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni83 – 853Substrate bindingBy similarity
    Regioni212 – 2132Substrate bindingBy similarity
    Regioni222 – 2232Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.Curated

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220467.
    KOiK01778.
    OMAiITIEMGT.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8NP73-1 [UniParc]FASTAAdd to Basket

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    MNLTIPFAKG HATENDFIII PDEDARLDLT PEMVVTLCDR RAGIGADGIL    50
    RVVKAADVEG STVDPSLWFM DYRNADGSLA EMCGNGVRLF AHWLYSRGLV 100
    DNTSFDIGTR AGVRHVDILQ ADQHSAQVRV DMGIPDVTGL STCDINGQVF 150
    AGLGVDMGNP HLACVVPGLS ASALADMELR APTFDQEFFP HGVNVEIVTE 200
    LEDDAVSMRV WERGVGETRS CGTGTVAAAC AALADAGLGE GTVKVCVPGG 250
    EVEVQIFDDG STLTGPSAII ALGEVQI 277
    Length:277
    Mass (Da):29,203
    Last modified:October 25, 2002 - v1
    Checksum:i41458032D8C7E9DF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000036 Genomic DNA. Translation: BAB99336.1.
    BX927153 Genomic DNA. Translation: CAF20284.1.
    RefSeqiNP_601150.1. NC_003450.3.
    YP_226185.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99336; BAB99336; BAB99336.
    CAF20284; CAF20284; cg2129.
    GeneIDi1019900.
    KEGGicgb:cg2129.
    cgl:NCgl1868.
    PATRICi21495890. VBICorGlu203724_1881.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000036 Genomic DNA. Translation: BAB99336.1 .
    BX927153 Genomic DNA. Translation: CAF20284.1 .
    RefSeqi NP_601150.1. NC_003450.3.
    YP_226185.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali Q8NP73.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg2129.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99336 ; BAB99336 ; BAB99336 .
    CAF20284 ; CAF20284 ; cg2129 .
    GeneIDi 1019900.
    KEGGi cgb:cg2129.
    cgl:NCgl1868.
    PATRICi 21495890. VBICorGlu203724_1881.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220467.
    KOi K01778.
    OMAi ITIEMGT.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. "Identification and characterization of the last two unknown genes, dapC and dapF, in the succinylase branch of the L-lysine biosynthesis of Corynebacterium glutamicum."
      Hartmann M., Tauch A., Eggeling L., Bathe B., Mockel B., Puhler A., Kalinowski J.
      J. Biotechnol. 104:199-211(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiDAPF_CORGL
    AccessioniPrimary (citable) accession number: Q8NP73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2002
    Last sequence update: October 25, 2002
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3