Q8NNZ4 (RNH2_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	Cgl2033, cg2230

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.
Sequence caution	The sequence CAF20373.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Ribonuclease HII HAMAP MF_00052_B		PRO_0000111569
Sites
	Metal binding	13	1	Divalent metal cation By similarity
	Metal binding	14	1	Divalent metal cation By similarity
	Metal binding	107	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NNZ4 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 63C0148B3E5FA225	FASTA	209	22,282
	10 20 30 40 50 60 MSRNGLGPVA GVDEAGRGAC CGPISIAACI LPDKPIQELA ALTDSKKLSA STREKLMPLI 70 80 90 100 110 120 KKHALAWSVI VISAQDIDRF GIQHANISGM RRAVAALGTQ PGYVLTDAMK VPGFTVPYLP 130 140 150 160 170 180 IIGGDASARC IAAASVLAKQ TRDDIMTDMA NDYPHYGLEI HKGYSTKIHM DAVRHHGASP 190 200 EHRYSYANVA KAHQEWLHAA DNDTTEGGA

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99426.1. BX927154 Genomic DNA. Translation: CAF20373.1. Different initiation.
RefSeq	NP_601238.1. NC_003450.3. YP_226274.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NNZ4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019989. 3345428.
GenomeReviews	Gene locus Cgl2033 in contig BA000036_GR. Gene locus cg2230 in contig BX927147_GR.
KEGG	cgb:cg2230. cgl:NCgl1957.
PATRIC	21496070. VBICorGlu203724_1971.
Phylogenomic databases
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	Q8NNZ4.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2230-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_CORGL

Accession

Primary (citable) accession number: Q8NNZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program