Q8NNX7 (TRMD_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Cgl2050, cg2249

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.
Sequence caution	The sequence BAB99443.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060365
Regions
	Region	90 – 123	34	Insert HAMAP MF_00605
	Region	181 – 186	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	157	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NNX7 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 816A508AD7E6E4A8	FASTA	289	31,791
	10 20 30 40 50 60 MTSSENLDSH RLRLDVVTIF PEYLDPLRHA LLGKAIEDGI LEVGVHDLRN WATGGHKAVD 70 80 90 100 110 120 DTPYGGGPGM VMKPEVWGPA LDDVAAGRVS GAELDSASLH LKNVRHDELG GVEKRAYVVE 130 140 150 160 170 180 EDRDLPLLLV PTPAGKPFTQ ADAQAWSNEE HIVFACGRYE GIDQRVIDDA ANRYRVREVS 190 200 210 220 230 240 IGDYVLIGGE VAVLVIAEAV VRLIPGVLGN RRSHEEDSFS DGLLEGPSYT KPRTWRGLDV 250 260 270 280 PEVLFSGNHA KVDRWRRDQA LLRTQAIRPE LIDASLLDST DLKVLGLDK

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99443.1. Different initiation. BX927154 Genomic DNA. Translation: CAF20388.1.
RefSeq	NP_601253.1. NC_003450.3. YP_226289.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NNX7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020004. 3343898.
GenomeReviews	Gene locus Cgl2050 in contig BA000036_GR. Gene locus cg2249 in contig BX927147_GR.
KEGG	cgb:cg2249. cgl:NCgl1972.
PATRIC	21496102. VBICorGlu203724_1987.
Phylogenomic databases
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	Q8NNX7.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2249-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. Divergent sequence. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 2 hits. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_CORGL

Accession

Primary (citable) accession number: Q8NNX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	December 6, 2002
Last modified:	January 25, 2012
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program