Q8NNV6 (RNC_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	Cgl2074, cg2273

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity. HAMAP MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity HAMAP MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro rRNA catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Ribonuclease 3 HAMAP MF_00104		PRO_0000180393
Regions
	Domain	21 – 149	129	RNase III
	Domain	176 – 244	69	DRBM

Sequences

	Sequence		Length	Mass (Da)
	Q8NNV6 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1CDB46E96F1279D8	FASTA	247	27,481
	10 20 30 40 50 60 MSRKKNRLTG VDALNEAFDA VDHQPLLDHL GVDIQRDLLV LALTHRSFAN ENGMLPNNER 70 80 90 100 110 120 LEFLGDAVLG LSVANKLYEQ YPSSPESDVS KMRASIVSRY GLADIAREID LGNHILLGKG 130 140 150 160 170 180 ELLTEGRSKD SILADTTEAL FGAIFRQHGF ETARDVILRL FAYKIDNASA RGIHQDWKTT 190 200 210 220 230 240 LQEELAQRKR PMAEYSATSV GPDHDLVFTA IVTLEGEEMG RGEGPNKKLA EQEAAHQAFR KLRESRA

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99467.1. BX927154 Genomic DNA. Translation: CAF20410.1.
RefSeq	NP_601274.1. NC_003450.3. YP_226311.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NNV6.
ModBase	Search...
2D gel databases
World-2DPAGE	0001:Q8NNV6.
Proteomic databases
PRIDE	Q8NNV6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020026. 3344302.
GenomeReviews	Gene locus Cgl2074 in contig BA000036_GR. Gene locus cg2273 in contig BX927147_GR.
KEGG	cgb:cg2273. cgl:NCgl1994.
PATRIC	21496148. VBICorGlu203724_2010.
Phylogenomic databases
HOGENOM	HBG744556.
OMA	ALTHKSF.
PhylomeDB	Q8NNV6.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2273-MONOMER.
Family and domain databases
HAMAP	MF_00104. RNase_III. [Tree]
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
KO	K03685.
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_CORGL

Accession

Primary (citable) accession number: Q8NNV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program