Q8NNR1 (Q8NNR1_CORGL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

Cgl2125

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	270	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	307	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	403	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	Q8NNR1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 501C18E977D616EA	FASTA	595	65,737
	10 20 30 40 50 60 MCHSISHLSS PTGSIFTSLV AMLTSQSFSV WAPLPHDVHL ILNGETLPMH KTEGSWWRAE 70 80 90 100 110 120 IAPKAGDRYG FSLFDGSSWS KTLPDPRSTS QPDGVHGLSE VSDDSYLWGD QQWTGRILPG 130 140 150 160 170 180 SVLYELHVGT FSEDGTFEGV VDKLPYLRDL GVTAIELLPV QPFGGNRNWG YDGVLWHAVH 190 200 210 220 230 240 AGYGGPAGLK KLIDASHQAG IAVYLDVVYN HFGPDGNYNG QFGPYTSGGS TGWGDVVNIN 250 260 270 280 290 300 GHDSDEVRNY ILDAARQWFE DFHVDGLRLD AVHSLDDRGA YSLLAQLTMV AEDVSAQTGI 310 320 330 340 350 360 PRSLIAESEL NDPKFVTSRE AGGFGLDAQW VDDIHHALHA LVSGERNGYY SDFGSVDTLA 370 380 390 400 410 420 KTLREVFEHT GNYSTYRGRN HGRPVHPDIT PASRFVTYTT THDQTGNRAI GDRPSTTLTP 430 440 450 460 470 480 EQQVLKAAII YSSPYTPMLF MGEEFGATTP FAFFCSHTDP ELNRLTSEGR KREFARLGWN 490 500 510 520 530 540 ADDIPSPELE STFTSSKLDW EFTAEQRRIN DAYKQLLHLR HTLGFSQPNL LTLEVEHGEN 550 560 570 580 590 WLSMANGRGR ILANFSDDTI TVPLGGELIY SFTSPTVTDT STTLQPWGFA ILTRN

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025 [Nakagawa].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99519.1.
RefSeq	NP_601327.2. NC_003450.3.
3D structure databases
HSSP	HSSP built from PDB template 1EH9 based on UniProtKB Q55088.
ProteinModelPortal	Q8NNR1.
ModBase	Search...
2D gel databases
World-2DPAGE	0001:Q8NNR1.
Proteomic databases
PRIDE	Q8NNR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020077.
GenomeReviews	Gene locus Cgl2125 in contig BA000036_GR.
KEGG	cgl:NCgl2045.
PATRIC	21496256. VBICorGlu203724_2064.
Phylogenomic databases
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK638715.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q8NNR1_CORGL

Accession

Primary (citable) accession number: Q8NNR1

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)