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Protein

DNA polymerase IV

Gene

dinB

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Sitei14Substrate discriminationUniRule annotation1
Metal bindingi104MagnesiumUniRule annotation1
Active sitei105UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCORYNE:G18NG-11736-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Synonyms:dinP
Ordered Locus Names:Cgl2144, cg2355
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739111 – 467DNA polymerase IVAdd BLAST467

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg2355

Structurei

3D structure databases

ProteinModelPortaliQ8NNP4
SMRiQ8NNP4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 187UmuCUniRule annotationAdd BLAST183

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3 Bacteria
COG0389 LUCA
HOGENOMiHOG000082707
KOiK02346
OMAiGMQIREI

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR022880 DNApol_IV
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

Q8NNP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRWVLHIDM DAFFASCEQL TRPTLRGRPV LVGGVSGRGV VAGASYEARK
60 70 80 90 100
FGARSAMPMH QAKARVGFGA VVVTPRHIVY SAASRRVFQI VEKRAGIVER
110 120 130 140 150
LSIDEGFMEP EALVGATPEE VKQWAEELRA EIKEVTGLPS SVGAGSGKQI
160 170 180 190 200
AKIGSGEAKP DGVFVVPVDK QHDLLDPLPV GALWGVGPVT GSKLASMGVE
210 220 230 240 250
TIGDLAALTQ KEVEISLGAT IGISLWNLAR GIDDRPVEPR AEAKQISQEH
260 270 280 290 300
TYEKDLLTRQ QVDAAIIRSA EGAHRRLLKD GRGARTVSVK LRMADFRIES
310 320 330 340 350
RSYTLSYATD DYATLEATAF RLARYPGEVG PIRLVGVSFS GLEESRQDIL
360 370 380 390 400
FPELDQQIIV PPAPDTDYEV GVQSSSSSES TQVEAPQDVA LSMWCATQDV
410 420 430 440 450
YHPEYGHGWV QGAGHGVVSV RFETRSTTKG RTKSFSMDDP DLTPADPLDS
460
LDWADWFAEN GETGDDE
Length:467
Mass (Da):50,688
Last modified:October 19, 2002 - v1
Checksum:i37B8F85D9D80CDDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA Translation: BAB99537.1
BX927154 Genomic DNA Translation: CAF20484.1
RefSeqiNP_601346.1, NC_003450.3
WP_011014912.1, NC_006958.1

Genome annotation databases

EnsemblBacteriaiBAB99537; BAB99537; BAB99537
CAF20484; CAF20484; cg2355
GeneIDi1020096
KEGGicgb:cg2355
cgl:NCgl2064
PATRICifig|196627.13.peg.2082

Similar proteinsi

Entry informationi

Entry nameiDPO4_CORGL
AccessioniPrimary (citable) accession number: Q8NNP4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health