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Protein

DNA polymerase IV

Gene

dinB

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91MagnesiumUniRule annotation
Sitei14 – 141Substrate discriminationUniRule annotation
Metal bindingi104 – 1041MagnesiumUniRule annotation
Active sitei105 – 1051UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Synonyms:dinP
Ordered Locus Names:Cgl2144, cg2355
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467DNA polymerase IVPRO_0000173911Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg2355.

Structurei

3D structure databases

ProteinModelPortaliQ8NNP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 187183UmuCUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation
Contains 1 umuC domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK02346.
OMAiCYVARIR.
OrthoDBiEOG6FZ4D8.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NNP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRWVLHIDM DAFFASCEQL TRPTLRGRPV LVGGVSGRGV VAGASYEARK
60 70 80 90 100
FGARSAMPMH QAKARVGFGA VVVTPRHIVY SAASRRVFQI VEKRAGIVER
110 120 130 140 150
LSIDEGFMEP EALVGATPEE VKQWAEELRA EIKEVTGLPS SVGAGSGKQI
160 170 180 190 200
AKIGSGEAKP DGVFVVPVDK QHDLLDPLPV GALWGVGPVT GSKLASMGVE
210 220 230 240 250
TIGDLAALTQ KEVEISLGAT IGISLWNLAR GIDDRPVEPR AEAKQISQEH
260 270 280 290 300
TYEKDLLTRQ QVDAAIIRSA EGAHRRLLKD GRGARTVSVK LRMADFRIES
310 320 330 340 350
RSYTLSYATD DYATLEATAF RLARYPGEVG PIRLVGVSFS GLEESRQDIL
360 370 380 390 400
FPELDQQIIV PPAPDTDYEV GVQSSSSSES TQVEAPQDVA LSMWCATQDV
410 420 430 440 450
YHPEYGHGWV QGAGHGVVSV RFETRSTTKG RTKSFSMDDP DLTPADPLDS
460
LDWADWFAEN GETGDDE
Length:467
Mass (Da):50,688
Last modified:October 19, 2002 - v1
Checksum:i37B8F85D9D80CDDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB99537.1.
BX927154 Genomic DNA. Translation: CAF20484.1.
RefSeqiNP_601346.1. NC_003450.3.
WP_011014912.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99537; BAB99537; BAB99537.
CAF20484; CAF20484; cg2355.
GeneIDi1020096.
KEGGicgb:cg2355.
cgl:NCgl2064.
PATRICi21496292. VBICorGlu203724_2082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB99537.1.
BX927154 Genomic DNA. Translation: CAF20484.1.
RefSeqiNP_601346.1. NC_003450.3.
WP_011014912.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NNP4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg2355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99537; BAB99537; BAB99537.
CAF20484; CAF20484; cg2355.
GeneIDi1020096.
KEGGicgb:cg2355.
cgl:NCgl2064.
PATRICi21496292. VBICorGlu203724_2082.

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK02346.
OMAiCYVARIR.
OrthoDBiEOG6FZ4D8.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiDPO4_CORGL
AccessioniPrimary (citable) accession number: Q8NNP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: November 11, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.