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Q8NNP0

- SYI_CORGL

UniProt

Q8NNP0 - SYI_CORGL

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei630 – 6301ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Cgl2148, cg2359
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10541054Isoleucine--tRNA ligasePRO_0000098538Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196627.cg2359.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NNP0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi627 – 6315"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8NNP0-1 [UniParc]FASTAAdd to Basket

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    MSEAVGGVYP QVDLSGGSSR FPEMEENVLS YWKKDDTFQA SIDQRDGAED     50
    YVFYDGPPFA NGLPHYGHLL TGYVKDIVPR YQTMRGYRVP RVFGWDTHGL 100
    PAELEAEKQL GIKDKGEIEA MGLAKFNEYC ATSVLQYTKE WEEYVTRQAR 150
    WVDFENGYKT MDLSFMESVI WAFKELYDKG LIYQGFRVLP YSWAEHTPLS 200
    NQETRLDDSY KLRQDPTLTV TFPVTGVVEG SSANAGLVGA LALAWTTTPW 250
    TLPSNLALAV NPAVTYALVE VAEDGEAEFV GKRVLLAKDL VGSYAKELGA 300
    EAVIVSEHPG SELVGLTYEP IFGYFRDHAN GFQILGAEYV TTEDGTGIVH 350
    QAPAFGEDDM NTCNAAGIEP VIPVDIDGKF TGLVPEYQGQ LVFDANKDII 400
    KDLKAAGRVV RHQTIEHSYP HSWRSGEPLI YMALPSWFVN VTEIRDRMVE 450
    VNQDIEWMPA HIRDGQFGKW LEGARDWNIS RSRYWGSPIP AWVSDNDEYP 500
    RVDVYGSLDE LEADFGVRPK SLHRPDIDEL TRPNPDDPTG KSTMRRVTDV 550
    LDVWFDSGSM PFAQVHYPFE NKEWFDTHAP ADFIVEYIGQ TRGWFYLLHV 600
    LSTALFDRPA FKKVVAHGIV LGDDGLKMSK SKGNYPNVNE VFDRDGSDAM 650
    RWFLMSSPIL RGGNLIVTEK GIREGVRQAQ LPMWNAYSFL QLYTSKNATW 700
    SVDSTDVLDR YILAKLHDLV AETQAALDGT DIAKACDLVR NFCDALTNWY 750
    VRRSRDRFWA GDEAHPEAFN TLYTVLETLT RVAAPLLPMT TEVIWRGLTG 800
    ERSVHLTDFP SAESFPADAD LVRTMDEIRG VCSAASSVRK AHKLRNRLPL 850
    PGLTVALPDS ARLADFASII RDEVNVKNVD LTSDVDSVGT FEVVVNAKVA 900
    GPRLGKDVQR VIKAVKAGNY TREGDVVVAD GIELNEGEFT ERLVAANPDS 950
    TAQIDGVDGL VVLDMEVTEE LEAEGWAADA IRGLQDARKN SGFEVSDRIS 1000
    VVVSVPEDKK EWITTHADHI AAEVLATSFE IVTDALDGET HDIVAGVTAK 1050
    VTKN 1054
    Length:1,054
    Mass (Da):117,404
    Last modified:October 1, 2002 - v1
    Checksum:iC3333C86C8554B1F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000036 Genomic DNA. Translation: BAB99541.1.
    BX927154 Genomic DNA. Translation: CAF20488.1.
    RefSeqiNP_601350.1. NC_003450.3.
    YP_226389.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99541; BAB99541; BAB99541.
    CAF20488; CAF20488; cg2359.
    GeneIDi1020100.
    KEGGicgb:cg2359.
    cgl:NCgl2068.
    PATRICi21496300. VBICorGlu203724_2086.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000036 Genomic DNA. Translation: BAB99541.1 .
    BX927154 Genomic DNA. Translation: CAF20488.1 .
    RefSeqi NP_601350.1. NC_003450.3.
    YP_226389.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali Q8NNP0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg2359.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99541 ; BAB99541 ; BAB99541 .
    CAF20488 ; CAF20488 ; cg2359 .
    GeneIDi 1020100.
    KEGGi cgb:cg2359.
    cgl:NCgl2068.
    PATRICi 21496300. VBICorGlu203724_2086.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiSYI_CORGL
    AccessioniPrimary (citable) accession number: Q8NNP0
    Secondary accession number(s): Q6M3S6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3