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Q8NNP0

- SYI_CORGL

UniProt

Q8NNP0 - SYI_CORGL

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Cgl2148, cg2359
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10541054Isoleucine--tRNA ligasePRO_0000098538Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg2359.

Structurei

3D structure databases

ProteinModelPortaliQ8NNP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi627 – 6315"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NNP0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEAVGGVYP QVDLSGGSSR FPEMEENVLS YWKKDDTFQA SIDQRDGAED
60 70 80 90 100
YVFYDGPPFA NGLPHYGHLL TGYVKDIVPR YQTMRGYRVP RVFGWDTHGL
110 120 130 140 150
PAELEAEKQL GIKDKGEIEA MGLAKFNEYC ATSVLQYTKE WEEYVTRQAR
160 170 180 190 200
WVDFENGYKT MDLSFMESVI WAFKELYDKG LIYQGFRVLP YSWAEHTPLS
210 220 230 240 250
NQETRLDDSY KLRQDPTLTV TFPVTGVVEG SSANAGLVGA LALAWTTTPW
260 270 280 290 300
TLPSNLALAV NPAVTYALVE VAEDGEAEFV GKRVLLAKDL VGSYAKELGA
310 320 330 340 350
EAVIVSEHPG SELVGLTYEP IFGYFRDHAN GFQILGAEYV TTEDGTGIVH
360 370 380 390 400
QAPAFGEDDM NTCNAAGIEP VIPVDIDGKF TGLVPEYQGQ LVFDANKDII
410 420 430 440 450
KDLKAAGRVV RHQTIEHSYP HSWRSGEPLI YMALPSWFVN VTEIRDRMVE
460 470 480 490 500
VNQDIEWMPA HIRDGQFGKW LEGARDWNIS RSRYWGSPIP AWVSDNDEYP
510 520 530 540 550
RVDVYGSLDE LEADFGVRPK SLHRPDIDEL TRPNPDDPTG KSTMRRVTDV
560 570 580 590 600
LDVWFDSGSM PFAQVHYPFE NKEWFDTHAP ADFIVEYIGQ TRGWFYLLHV
610 620 630 640 650
LSTALFDRPA FKKVVAHGIV LGDDGLKMSK SKGNYPNVNE VFDRDGSDAM
660 670 680 690 700
RWFLMSSPIL RGGNLIVTEK GIREGVRQAQ LPMWNAYSFL QLYTSKNATW
710 720 730 740 750
SVDSTDVLDR YILAKLHDLV AETQAALDGT DIAKACDLVR NFCDALTNWY
760 770 780 790 800
VRRSRDRFWA GDEAHPEAFN TLYTVLETLT RVAAPLLPMT TEVIWRGLTG
810 820 830 840 850
ERSVHLTDFP SAESFPADAD LVRTMDEIRG VCSAASSVRK AHKLRNRLPL
860 870 880 890 900
PGLTVALPDS ARLADFASII RDEVNVKNVD LTSDVDSVGT FEVVVNAKVA
910 920 930 940 950
GPRLGKDVQR VIKAVKAGNY TREGDVVVAD GIELNEGEFT ERLVAANPDS
960 970 980 990 1000
TAQIDGVDGL VVLDMEVTEE LEAEGWAADA IRGLQDARKN SGFEVSDRIS
1010 1020 1030 1040 1050
VVVSVPEDKK EWITTHADHI AAEVLATSFE IVTDALDGET HDIVAGVTAK

VTKN
Length:1,054
Mass (Da):117,404
Last modified:October 1, 2002 - v1
Checksum:iC3333C86C8554B1F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB99541.1.
BX927154 Genomic DNA. Translation: CAF20488.1.
RefSeqiNP_601350.1. NC_003450.3.
YP_226389.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99541; BAB99541; BAB99541.
CAF20488; CAF20488; cg2359.
GeneIDi1020100.
3345452.
KEGGicgb:cg2359.
cgl:NCgl2068.
PATRICi21496300. VBICorGlu203724_2086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB99541.1 .
BX927154 Genomic DNA. Translation: CAF20488.1 .
RefSeqi NP_601350.1. NC_003450.3.
YP_226389.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q8NNP0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg2359.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99541 ; BAB99541 ; BAB99541 .
CAF20488 ; CAF20488 ; cg2359 .
GeneIDi 1020100.
3345452.
KEGGi cgb:cg2359.
cgl:NCgl2068.
PATRICi 21496300. VBICorGlu203724_2086.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiSYI_CORGL
AccessioniPrimary (citable) accession number: Q8NNP0
Secondary accession number(s): Q6M3S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3