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Q8NNP0

- SYI_CORGL

UniProt

Q8NNP0 - SYI_CORGL

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Cgl2148, cg2359
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Cgl2148, cg2359
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10541054Isoleucine--tRNA ligaseUniRule annotationPRO_0000098538Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg2359.

Structurei

3D structure databases

ProteinModelPortaliQ8NNP0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi627 – 6315"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NNP0-1 [UniParc]FASTAAdd to Basket

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MSEAVGGVYP QVDLSGGSSR FPEMEENVLS YWKKDDTFQA SIDQRDGAED     50
YVFYDGPPFA NGLPHYGHLL TGYVKDIVPR YQTMRGYRVP RVFGWDTHGL 100
PAELEAEKQL GIKDKGEIEA MGLAKFNEYC ATSVLQYTKE WEEYVTRQAR 150
WVDFENGYKT MDLSFMESVI WAFKELYDKG LIYQGFRVLP YSWAEHTPLS 200
NQETRLDDSY KLRQDPTLTV TFPVTGVVEG SSANAGLVGA LALAWTTTPW 250
TLPSNLALAV NPAVTYALVE VAEDGEAEFV GKRVLLAKDL VGSYAKELGA 300
EAVIVSEHPG SELVGLTYEP IFGYFRDHAN GFQILGAEYV TTEDGTGIVH 350
QAPAFGEDDM NTCNAAGIEP VIPVDIDGKF TGLVPEYQGQ LVFDANKDII 400
KDLKAAGRVV RHQTIEHSYP HSWRSGEPLI YMALPSWFVN VTEIRDRMVE 450
VNQDIEWMPA HIRDGQFGKW LEGARDWNIS RSRYWGSPIP AWVSDNDEYP 500
RVDVYGSLDE LEADFGVRPK SLHRPDIDEL TRPNPDDPTG KSTMRRVTDV 550
LDVWFDSGSM PFAQVHYPFE NKEWFDTHAP ADFIVEYIGQ TRGWFYLLHV 600
LSTALFDRPA FKKVVAHGIV LGDDGLKMSK SKGNYPNVNE VFDRDGSDAM 650
RWFLMSSPIL RGGNLIVTEK GIREGVRQAQ LPMWNAYSFL QLYTSKNATW 700
SVDSTDVLDR YILAKLHDLV AETQAALDGT DIAKACDLVR NFCDALTNWY 750
VRRSRDRFWA GDEAHPEAFN TLYTVLETLT RVAAPLLPMT TEVIWRGLTG 800
ERSVHLTDFP SAESFPADAD LVRTMDEIRG VCSAASSVRK AHKLRNRLPL 850
PGLTVALPDS ARLADFASII RDEVNVKNVD LTSDVDSVGT FEVVVNAKVA 900
GPRLGKDVQR VIKAVKAGNY TREGDVVVAD GIELNEGEFT ERLVAANPDS 950
TAQIDGVDGL VVLDMEVTEE LEAEGWAADA IRGLQDARKN SGFEVSDRIS 1000
VVVSVPEDKK EWITTHADHI AAEVLATSFE IVTDALDGET HDIVAGVTAK 1050
VTKN 1054
Length:1,054
Mass (Da):117,404
Last modified:October 1, 2002 - v1
Checksum:iC3333C86C8554B1F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB99541.1.
BX927154 Genomic DNA. Translation: CAF20488.1.
RefSeqiNP_601350.1. NC_003450.3.
YP_226389.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99541; BAB99541; BAB99541.
CAF20488; CAF20488; cg2359.
GeneIDi1020100.
KEGGicgb:cg2359.
cgl:NCgl2068.
PATRICi21496300. VBICorGlu203724_2086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB99541.1 .
BX927154 Genomic DNA. Translation: CAF20488.1 .
RefSeqi NP_601350.1. NC_003450.3.
YP_226389.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q8NNP0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg2359.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99541 ; BAB99541 ; BAB99541 .
CAF20488 ; CAF20488 ; cg2359 .
GeneIDi 1020100.
KEGGi cgb:cg2359.
cgl:NCgl2068.
PATRICi 21496300. VBICorGlu203724_2086.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiSYI_CORGL
AccessioniPrimary (citable) accession number: Q8NNP0
Secondary accession number(s): Q6M3S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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