Q8NNM7 (RSMH_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	Cgl2166, cg2377

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108615
Regions
	Region	45 – 47	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	64	1	S-adenosyl-L-methionine By similarity
	Binding site	91	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	120	1	S-adenosyl-L-methionine By similarity
	Binding site	127	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NNM7 [UniParc]. Last modified October 19, 2002. Version 1. Checksum: A16871B75759BF9A	FASTA	337	36,809
	10 20 30 40 50 60 MEDFSLDGNH GHVPVMRDRM AALIAEHVEA LGENAVIVDA TLGAGGHAEF FLNTFPKARL 70 80 90 100 110 120 IGLDRDQNAL RDARARLAPF GERFIGVQTR FDGLREVLES VEGDIIDLAR EHGIAGALFD 130 140 150 160 170 180 LGVSSMQLDQ VERGFAYRTD APLDMRMDAT QGITAADILN TYSHGDIARI LKTYGDERFA 190 200 210 220 230 240 GKIASAVLKE REKEPFTTSA RLVELLYDAI PAATRRTGGH PAKRTFQALR VEVNNELDSL 250 260 270 280 290 300 KNVLPQITDA LNVGGRAVFM SYQSHEDKLV KKFFTDLTTS KTPPGLPVDL PGTAPQFKQV 310 320 330 TRGAETASEA EIEENPRAAP VKVRAIERIG NNSGDLS

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99559.1. BX927154 Genomic DNA. Translation: CAF20506.1.
RefSeq	NP_601368.1. NC_003450.3. YP_226407.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NNM7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020118. 3342863.
GenomeReviews	Gene locus Cgl2166 in contig BA000036_GR. Gene locus cg2377 in contig BX927147_GR.
KEGG	cgb:cg2377. cgl:NCgl2086.
PATRIC	21496336. VBICorGlu203724_2104.
Phylogenomic databases
HOGENOM	HBG302779.
OMA	GHVPVMR.
PhylomeDB	Q8NNM7.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2377-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_CORGL

Accession

Primary (citable) accession number: Q8NNM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program