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Protein

GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase

Gene

pimB

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of a mannose residue from GDP-D-mannose to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (PIM2). Can also add a mannose residue to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol mannoside (PIM1).1 Publication

Catalytic activityi

Transfers one or more alpha-D-mannose residues from GDP-mannose to positions 2,6 and others in 1-phosphatidyl-myo-inositol.

Pathwayi: phosphatidylinositol metabolism

This protein is involved in the pathway phosphatidylinositol metabolism, which is part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the pathway phosphatidylinositol metabolism and in Phospholipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201Substrate; via amide nitrogenBy similarity
Sitei118 – 1181Important for catalytic activityBy similarity
Binding sitei206 – 2061SubstrateBy similarity
Binding sitei261 – 2611SubstrateBy similarity
Binding sitei265 – 2651SubstrateBy similarity
Sitei290 – 2901Important for catalytic activityBy similarity
Binding sitei298 – 2981SubstrateBy similarity

GO - Molecular functioni

  • glycolipid 6-alpha-mannosyltransferase activity Source: UniProtKB
  • phosphatidylinositol alpha-mannosyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00949.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase (EC:2.4.1.57)
Alternative name(s):
Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferase
Alpha-mannosyltransferase
Short name:
Alpha-ManT
Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase
Phosphatidylinositol alpha-mannosyltransferase
Short name:
PI alpha-mannosyltransferase
Gene namesi
Name:pimB
Ordered Locus Names:Cgl2186, cg2400
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferasePRO_0000393734Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196627.cg2400.

Structurei

Secondary structure

1
381
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Helixi20 – 2910Combined sources
Helixi34 – 363Combined sources
Beta strandi37 – 426Combined sources
Helixi46 – 538Combined sources
Beta strandi57 – 6711Combined sources
Helixi72 – 8413Combined sources
Beta strandi88 – 947Combined sources
Helixi98 – 1014Combined sources
Helixi102 – 1076Combined sources
Beta strandi111 – 1166Combined sources
Helixi121 – 1244Combined sources
Helixi128 – 14013Combined sources
Beta strandi142 – 1476Combined sources
Helixi149 – 15911Combined sources
Beta strandi161 – 1677Combined sources
Turni174 – 1763Combined sources
Helixi182 – 19110Combined sources
Beta strandi200 – 2056Combined sources
Helixi209 – 2113Combined sources
Helixi213 – 22614Combined sources
Beta strandi231 – 2355Combined sources
Helixi241 – 2477Combined sources
Helixi249 – 2546Combined sources
Beta strandi255 – 2606Combined sources
Helixi263 – 27210Combined sources
Beta strandi274 – 2785Combined sources
Helixi284 – 2863Combined sources
Helixi294 – 3018Combined sources
Beta strandi306 – 3083Combined sources
Helixi314 – 3174Combined sources
Turni320 – 3223Combined sources
Beta strandi323 – 3253Combined sources
Helixi331 – 34212Combined sources
Helixi345 – 36218Combined sources
Helixi365 – 37713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OKAX-ray2.20A/B1-381[»]
3OKCX-ray2.40A1-381[»]
3OKPX-ray2.00A1-381[»]
ProteinModelPortaliQ8NNK8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NNK8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 2112Substrate bindingBy similarity
Regioni290 – 2945Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105JNK. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077286.
KOiK13668.
OMAiRSDHVTA.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NNK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASRKTLVV TNDFPPRIGG IQSYLRDFIA TQDPESIVVF ASTQNAEEAH
60 70 80 90 100
AYDKTLDYEV IRWPRSVMLP TPTTAHAMAE IIREREIDNV WFGAAAPLAL
110 120 130 140 150
MAGTAKQAGA SKVIASTHGH EVGWSMLPGS RQSLRKIGTE VDVLTYISQY
160 170 180 190 200
TLRRFKSAFG SHPTFEHLPS GVDVKRFTPA TPEDKSATRK KLGFTDTTPV
210 220 230 240 250
IACNSRLVPR KGQDSLIKAM PQVIAARPDA QLLIVGSGRY ESTLRRLATD
260 270 280 290 300
VSQNVKFLGR LEYQDMINTL AAADIFAMPA RTRGGGLDVE GLGIVYLEAQ
310 320 330 340 350
ACGVPVIAGT SGGAPETVTP ATGLVVEGSD VDKLSELLIE LLDDPIRRAA
360 370 380
MGAAGRAHVE AEWSWEIMGE RLTNILQSEP R
Length:381
Mass (Da):41,328
Last modified:October 1, 2002 - v1
Checksum:i99AECD1E156444DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB99579.1.
BX927154 Genomic DNA. Translation: CAF20527.1.
RefSeqiNP_601390.1. NC_003450.3.
WP_011014943.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99579; BAB99579; BAB99579.
CAF20527; CAF20527; cg2400.
GeneIDi1020138.
KEGGicgb:cg2400.
cgl:NCgl2106.
PATRICi21496374. VBICorGlu203724_2123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB99579.1.
BX927154 Genomic DNA. Translation: CAF20527.1.
RefSeqiNP_601390.1. NC_003450.3.
WP_011014943.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OKAX-ray2.20A/B1-381[»]
3OKCX-ray2.40A1-381[»]
3OKPX-ray2.00A1-381[»]
ProteinModelPortaliQ8NNK8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg2400.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99579; BAB99579; BAB99579.
CAF20527; CAF20527; cg2400.
GeneIDi1020138.
KEGGicgb:cg2400.
cgl:NCgl2106.
PATRICi21496374. VBICorGlu203724_2123.

Phylogenomic databases

eggNOGiENOG4105JNK. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077286.
KOiK13668.
OMAiRSDHVTA.

Enzyme and pathway databases

UniPathwayiUPA00949.

Miscellaneous databases

EvolutionaryTraceiQ8NNK8.
PROiQ8NNK8.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIMB_CORGL
AccessioniPrimary (citable) accession number: Q8NNK8
Secondary accession number(s): Q6M3P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.