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Protein

Cytochrome c oxidase subunit 2

Gene

ctaC

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B) (By similarity).By similarity

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactori

binuclear copper center (CuA)By similarityNote: Binds a binuclear copper A center per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei29 – 291Not N-palmitoylated
Metal bindingi244 – 2441Copper A1By similarity
Metal bindingi285 – 2851Copper A1By similarity
Metal bindingi285 – 2851Copper A2By similarity
Metal bindingi287 – 2871Copper A2; via carbonyl oxygenBy similarity
Metal bindingi289 – 2891Copper A1By similarity
Metal bindingi289 – 2891Copper A2By similarity
Metal bindingi293 – 2931Copper A2By similarity
Metal bindingi296 – 2961Copper A1By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. cytochrome-c oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. electron transport chain Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-2169-MONOMER.

Protein family/group databases

TCDBi3.D.4.4.2. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 2 (EC:1.9.3.1)
Alternative name(s):
Cytochrome aa3 subunit 2
Cytochrome c oxidase polypeptide II
Oxidase aa(3) subunit 2
Gene namesi
Name:ctaC
Ordered Locus Names:Cgl2195, cg2409
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001009 Componenti: Chromosome UP000000582 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei64 – 8421HelicalSequence AnalysisAdd
BLAST
Transmembranei107 – 12721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
  3. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 359331Cytochrome c oxidase subunit 2PRO_0000006054Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi29 – 291S-diacylglycerol cysteine1 Publication

Keywords - PTMi

Lipoprotein

Interactioni

Subunit structurei

Associates with subunits I, III and IV to form cytochrome c oxidase. The 4 subunit cytochrome c oxidase forms a supercomplex with the menaquinol-cytochrome c reductase complex (cytochrome bc1).1 Publication

Protein-protein interaction databases

STRINGi196627.cg2409.

Structurei

3D structure databases

ProteinModelPortaliQ8NNK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1622.
HOGENOMiHOG000245527.
KOiK02275.
OMAiVLRFGWP.
OrthoDBiEOG68SVXT.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 2 hits.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 2 hits.
SSF81464. SSF81464. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NNK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQQNKRGLK RKALLGGVLG LGGLAMAGCE VAPPGGVLGD FLRMGWPDGI
60 70 80 90 100
TPEAVAMGNF WSWVWVAAWI IGIIMWGLFL TAIFAWGAKR AEKRGEGEFP
110 120 130 140 150
KQLQYNVPLE LVLTIVPIII VMVLFFFTVQ TQDKVTALDK NPEVTVDVTA
160 170 180 190 200
YQWNWKFGYS EIDGSLAPGG QDYQGSDPER QAAAEASKKD PSGDNPIHGN
210 220 230 240 250
SKSDVSYLEF NRIETLGTTD EIPVMVLPVN TPIEFNLASA DVAHSFWVPE
260 270 280 290 300
FLFKRDAYAH PEANKSQRVF QIEEITEEGA FVGRCAEMCG TYHAMMNFEL
310 320 330 340 350
RVVDRDSFAE YISFRDSNPD ATNAQALEHI GQAPYATSTS PFVSDRTATR

DGENTQSNA
Length:359
Mass (Da):39,632
Last modified:October 1, 2002 - v1
Checksum:i5BB65F6AB2BE493A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691W → C in BAB64407 (PubMed:11577165).Curated
Sequence conflicti310 – 3101E → V in BAB64407 (PubMed:11577165).Curated

Mass spectrometryi

Molecular mass is 37314.7±83.2 Da from positions 29 - 359. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052749 Genomic DNA. Translation: BAB64407.1.
BA000036 Genomic DNA. Translation: BAB99588.1.
BX927154 Genomic DNA. Translation: CAF20536.1.
RefSeqiNP_601399.1. NC_003450.3.
YP_226437.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99588; BAB99588; BAB99588.
GeneIDi1020147.
KEGGicgb:cg2409.
cgl:NCgl2115.
PATRICi21496390. VBICorGlu203724_2131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052749 Genomic DNA. Translation: BAB64407.1.
BA000036 Genomic DNA. Translation: BAB99588.1.
BX927154 Genomic DNA. Translation: CAF20536.1.
RefSeqiNP_601399.1. NC_003450.3.
YP_226437.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NNK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg2409.

Protein family/group databases

TCDBi3.D.4.4.2. the proton-translocating cytochrome oxidase (cox) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99588; BAB99588; BAB99588.
GeneIDi1020147.
KEGGicgb:cg2409.
cgl:NCgl2115.
PATRICi21496390. VBICorGlu203724_2131.

Phylogenomic databases

eggNOGiCOG1622.
HOGENOMiHOG000245527.
KOiK02275.
OMAiVLRFGWP.
OrthoDBiEOG68SVXT.

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-2169-MONOMER.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 2 hits.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 2 hits.
SSF81464. SSF81464. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cytochrome c oxidase contains an extra charged amino acid cluster in a new type of respiratory chain in the amino acid-producing Gram-positive bacterium Corynebacterium glutamicum."
    Sakamoto J., Shibata T., Mine T., Miyahara R., Torigoe T., Noguchi S., Matsushita K., Sone N.
    Microbiology 147:2865-2871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 29-50, SUBUNIT, HEME CHARACTERIZATION, DIACYLGLYCEROL AT CYS-29, MASS SPECTROMETRY.
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. "Purification of a cytochrome bc1-aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum. Identification of a fourth subunity of cytochrome aa3 oxidase and mutational analysis of diheme cytochrome c1."
    Niebisch A., Bott M.
    J. Biol. Chem. 278:4339-4346(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DETECTION IN A SUPERCOMPLEX WITH MENAQUINOL-CYTOCHROME C REDUCTASE (CYTOCHROME BC1).
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiCOX2_CORGL
AccessioniPrimary (citable) accession number: Q8NNK2
Secondary accession number(s): Q6M3N8, Q93HZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2002
Last modified: April 1, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.