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Q8NNK2

- COX2_CORGL

UniProt

Q8NNK2 - COX2_CORGL

Protein

Cytochrome c oxidase subunit 2

Gene

ctaC

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B) By similarity.By similarity

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Cofactori

    Binds 2 copper A ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei29 – 291Not N-palmitoylated
    Metal bindingi244 – 2441Copper A1By similarity
    Metal bindingi285 – 2851Copper A1By similarity
    Metal bindingi285 – 2851Copper A2By similarity
    Metal bindingi287 – 2871Copper A2; via carbonyl oxygenBy similarity
    Metal bindingi289 – 2891Copper A1By similarity
    Metal bindingi289 – 2891Copper A2By similarity
    Metal bindingi293 – 2931Copper A2By similarity
    Metal bindingi296 – 2961Copper A1By similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. cytochrome-c oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. electron transport chain Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    BioCyciCGLU196627:GJDM-2169-MONOMER.

    Protein family/group databases

    TCDBi3.D.4.4.2. the proton-translocating cytochrome oxidase (cox) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 2 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome aa3 subunit 2
    Cytochrome c oxidase polypeptide II
    Oxidase aa(3) subunit 2
    Gene namesi
    Name:ctaC
    Ordered Locus Names:Cgl2195, cg2409
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 PublicationAdd
    BLAST
    Chaini29 – 359331Cytochrome c oxidase subunit 2PRO_0000006054Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi29 – 291S-diacylglycerol cysteine1 Publication

    Keywords - PTMi

    Lipoprotein

    Interactioni

    Subunit structurei

    Associates with subunits I, III and IV to form cytochrome c oxidase. The 4 subunit cytochrome c oxidase forms a supercomplex with the menaquinol-cytochrome c reductase complex (cytochrome bc1).1 Publication

    Protein-protein interaction databases

    STRINGi196627.cg2409.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NNK2.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei64 – 8421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei107 – 12721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1622.
    HOGENOMiHOG000245527.
    KOiK02275.
    OMAiMRELWTW.
    OrthoDBiEOG68SVXT.

    Family and domain databases

    Gene3Di1.10.287.90. 1 hit.
    2.60.40.420. 2 hits.
    InterProiIPR001505. Copper_CuA.
    IPR008972. Cupredoxin.
    IPR002429. Cyt_c_oxidase_su2_C.
    IPR011759. Cyt_c_oxidase_su2_TM_dom.
    [Graphical view]
    PfamiPF00116. COX2. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 2 hits.
    SSF81464. SSF81464. 1 hit.
    PROSITEiPS00078. COX2. 1 hit.
    PS50857. COX2_CUA. 1 hit.
    PS50999. COX2_TM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8NNK2-1 [UniParc]FASTAAdd to Basket

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    MEQQNKRGLK RKALLGGVLG LGGLAMAGCE VAPPGGVLGD FLRMGWPDGI    50
    TPEAVAMGNF WSWVWVAAWI IGIIMWGLFL TAIFAWGAKR AEKRGEGEFP 100
    KQLQYNVPLE LVLTIVPIII VMVLFFFTVQ TQDKVTALDK NPEVTVDVTA 150
    YQWNWKFGYS EIDGSLAPGG QDYQGSDPER QAAAEASKKD PSGDNPIHGN 200
    SKSDVSYLEF NRIETLGTTD EIPVMVLPVN TPIEFNLASA DVAHSFWVPE 250
    FLFKRDAYAH PEANKSQRVF QIEEITEEGA FVGRCAEMCG TYHAMMNFEL 300
    RVVDRDSFAE YISFRDSNPD ATNAQALEHI GQAPYATSTS PFVSDRTATR 350
    DGENTQSNA 359
    Length:359
    Mass (Da):39,632
    Last modified:October 1, 2002 - v1
    Checksum:i5BB65F6AB2BE493A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691W → C in BAB64407. (PubMed:11577165)Curated
    Sequence conflicti310 – 3101E → V in BAB64407. (PubMed:11577165)Curated

    Mass spectrometryi

    Molecular mass is 37314.7±83.2 Da from positions 29 - 359. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB052749 Genomic DNA. Translation: BAB64407.1.
    BA000036 Genomic DNA. Translation: BAB99588.1.
    BX927154 Genomic DNA. Translation: CAF20536.1.
    RefSeqiNP_601399.1. NC_003450.3.
    YP_226437.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99588; BAB99588; BAB99588.
    CAF20536; CAF20536; cg2409.
    GeneIDi1020147.
    KEGGicgb:cg2409.
    cgl:NCgl2115.
    PATRICi21496390. VBICorGlu203724_2131.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB052749 Genomic DNA. Translation: BAB64407.1 .
    BA000036 Genomic DNA. Translation: BAB99588.1 .
    BX927154 Genomic DNA. Translation: CAF20536.1 .
    RefSeqi NP_601399.1. NC_003450.3.
    YP_226437.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali Q8NNK2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg2409.

    Protein family/group databases

    TCDBi 3.D.4.4.2. the proton-translocating cytochrome oxidase (cox) superfamily.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99588 ; BAB99588 ; BAB99588 .
    CAF20536 ; CAF20536 ; cg2409 .
    GeneIDi 1020147.
    KEGGi cgb:cg2409.
    cgl:NCgl2115.
    PATRICi 21496390. VBICorGlu203724_2131.

    Phylogenomic databases

    eggNOGi COG1622.
    HOGENOMi HOG000245527.
    KOi K02275.
    OMAi MRELWTW.
    OrthoDBi EOG68SVXT.

    Enzyme and pathway databases

    BioCyci CGLU196627:GJDM-2169-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.90. 1 hit.
    2.60.40.420. 2 hits.
    InterProi IPR001505. Copper_CuA.
    IPR008972. Cupredoxin.
    IPR002429. Cyt_c_oxidase_su2_C.
    IPR011759. Cyt_c_oxidase_su2_TM_dom.
    [Graphical view ]
    Pfami PF00116. COX2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 2 hits.
    SSF81464. SSF81464. 1 hit.
    PROSITEi PS00078. COX2. 1 hit.
    PS50857. COX2_CUA. 1 hit.
    PS50999. COX2_TM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cytochrome c oxidase contains an extra charged amino acid cluster in a new type of respiratory chain in the amino acid-producing Gram-positive bacterium Corynebacterium glutamicum."
      Sakamoto J., Shibata T., Mine T., Miyahara R., Torigoe T., Noguchi S., Matsushita K., Sone N.
      Microbiology 147:2865-2871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 29-50, SUBUNIT, HEME CHARACTERIZATION, DIACYLGLYCEROL AT CYS-29, MASS SPECTROMETRY.
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    4. "Purification of a cytochrome bc1-aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum. Identification of a fourth subunity of cytochrome aa3 oxidase and mutational analysis of diheme cytochrome c1."
      Niebisch A., Bott M.
      J. Biol. Chem. 278:4339-4346(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DETECTION IN A SUPERCOMPLEX WITH MENAQUINOL-CYTOCHROME C REDUCTASE (CYTOCHROME BC1).
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiCOX2_CORGL
    AccessioniPrimary (citable) accession number: Q8NNK2
    Secondary accession number(s): Q6M3N8, Q93HZ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3