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Q8NNK2 (COX2_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome c oxidase subunit 2

EC=1.9.3.1
Alternative name(s):
Cytochrome aa3 subunit 2
Cytochrome c oxidase polypeptide II
Oxidase aa(3) subunit 2
Gene names
Name:ctaC
Ordered Locus Names:Cgl2195, cg2409
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B) By similarity.

Catalytic activity

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactor

Binds 2 copper A ions per subunit By similarity.

Subunit structure

Associates with subunits I, III and IV to form cytochrome c oxidase. The 4 subunit cytochrome c oxidase forms a supercomplex with the menaquinol-cytochrome c reductase complex (cytochrome bc1). Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cytochrome c oxidase subunit 2 family.

Mass spectrometry

Molecular mass is 37314.7±83.2 Da from positions 29 - 359. Determined by MALDI. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.1
Chain29 – 359331Cytochrome c oxidase subunit 2
PRO_0000006054

Regions

Transmembrane64 – 8421Helical; Potential
Transmembrane107 – 12721Helical; Potential

Sites

Metal binding2441Copper A1 By similarity
Metal binding2851Copper A1 By similarity
Metal binding2851Copper A2 By similarity
Metal binding2871Copper A2; via carbonyl oxygen By similarity
Metal binding2891Copper A1 By similarity
Metal binding2891Copper A2 By similarity
Metal binding2931Copper A2 By similarity
Metal binding2961Copper A1 By similarity
Site291Not N-palmitoylated

Amino acid modifications

Lipidation291S-diacylglycerol cysteine

Experimental info

Sequence conflict691W → C in BAB64407. Ref.1
Sequence conflict3101E → V in BAB64407. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8NNK2 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 5BB65F6AB2BE493A

FASTA35939,632
        10         20         30         40         50         60 
MEQQNKRGLK RKALLGGVLG LGGLAMAGCE VAPPGGVLGD FLRMGWPDGI TPEAVAMGNF 

        70         80         90        100        110        120 
WSWVWVAAWI IGIIMWGLFL TAIFAWGAKR AEKRGEGEFP KQLQYNVPLE LVLTIVPIII 

       130        140        150        160        170        180 
VMVLFFFTVQ TQDKVTALDK NPEVTVDVTA YQWNWKFGYS EIDGSLAPGG QDYQGSDPER 

       190        200        210        220        230        240 
QAAAEASKKD PSGDNPIHGN SKSDVSYLEF NRIETLGTTD EIPVMVLPVN TPIEFNLASA 

       250        260        270        280        290        300 
DVAHSFWVPE FLFKRDAYAH PEANKSQRVF QIEEITEEGA FVGRCAEMCG TYHAMMNFEL 

       310        320        330        340        350 
RVVDRDSFAE YISFRDSNPD ATNAQALEHI GQAPYATSTS PFVSDRTATR DGENTQSNA 

« Hide

References

« Hide 'large scale' references
[1]"Cytochrome c oxidase contains an extra charged amino acid cluster in a new type of respiratory chain in the amino acid-producing Gram-positive bacterium Corynebacterium glutamicum."
Sakamoto J., Shibata T., Mine T., Miyahara R., Torigoe T., Noguchi S., Matsushita K., Sone N.
Microbiology 147:2865-2871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 29-50, SUBUNIT, HEME CHARACTERIZATION, DIACYLGLYCEROL AT CYS-29, MASS SPECTROMETRY.
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]"Purification of a cytochrome bc1-aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum. Identification of a fourth subunity of cytochrome aa3 oxidase and mutational analysis of diheme cytochrome c1."
Niebisch A., Bott M.
J. Biol. Chem. 278:4339-4346(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DETECTION IN A SUPERCOMPLEX WITH MENAQUINOL-CYTOCHROME C REDUCTASE (CYTOCHROME BC1).
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB052749 Genomic DNA. Translation: BAB64407.1.
BA000036 Genomic DNA. Translation: BAB99588.1.
BX927154 Genomic DNA. Translation: CAF20536.1.
RefSeqNP_601399.1. NC_003450.3.
YP_226437.1. NC_006958.1.

3D structure databases

ProteinModelPortalQ8NNK2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg2409.

Protein family/group databases

TCDB3.D.4.4.2. the proton-translocating cytochrome oxidase (cox) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB99588; BAB99588; BAB99588.
CAF20536; CAF20536; cg2409.
GeneID1020147.
3345096.
KEGGcgb:cg2409.
cgl:NCgl2115.
PATRIC21496390. VBICorGlu203724_2131.

Phylogenomic databases

eggNOGCOG1622.
HOGENOMHOG000245527.
KOK02275.
OMAMNFEVRA.
OrthoDBEOG68SVXT.
ProtClustDBCLSK2518737.

Enzyme and pathway databases

BioCycCGLU196627:GJDM-2169-MONOMER.

Family and domain databases

Gene3D1.10.287.90. 1 hit.
2.60.40.420. 2 hits.
InterProIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamPF00116. COX2. 1 hit.
[Graphical view]
SUPFAMSSF49503. SSF49503. 2 hits.
SSF81464. SSF81464. 1 hit.
PROSITEPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCOX2_CORGL
AccessionPrimary (citable) accession number: Q8NNK2
Secondary accession number(s): Q6M3N8, Q93HZ4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families