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Protein

Malate dehydrogenase

Gene

mdh

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate. Exhibits higher catalytic efficiency for oxaloacetate reduction than for malate oxidation in vitro. Almost equally active both for NADH and NADPH on the bases of the kcat values at pH 6.5, but catalytic efficiency for oxaloacetate reduction is 50-fold higher with NADH.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation1 Publication

Enzyme regulationi

Citrate activates the enzyme in the oxidation of malate to oxaloacetate and inhibits it in the reverse reaction.1 Publication

Kineticsi

kcat is 360 sec(-1) for NADH-dependent reduction of oxaloacetate. kcat is 150 sec(-1) for NADPH-dependent reduction of oxaloacetate. kcat is 140 sec(-1) for NAD+-dependent oxidation of malate.1 Publication

  1. KM=0.025 mM for oxaloacetate (at pH 6.5 in the presence of NADH)1 Publication
  2. KM=0.55 mM for oxaloacetate (at pH 6.5 in the presence of NADPH)1 Publication
  3. KM=0.07 mM for NADH (at pH 6.5)1 Publication
  4. KM=0.25 mM for NADPH (at pH 6.5)1 Publication
  5. KM=0.8 mM for malate (at pH 10.5 in the presence of NAD)1 Publication
  6. KM=0.4 mM for NAD (at pH 10.5)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei97 – 971SubstrateUniRule annotation
    Binding sitei103 – 1031SubstrateUniRule annotation
    Binding sitei110 – 1101NADUniRule annotation
    Binding sitei117 – 1171NADUniRule annotation
    Binding sitei136 – 1361SubstrateUniRule annotation
    Binding sitei167 – 1671SubstrateUniRule annotation
    Active sitei192 – 1921Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 227NADUniRule annotation
    Nucleotide bindingi134 – 1363NADUniRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi1.1.1.37. 960.
    SABIO-RKQ8NN33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation1 Publication)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:Cgl2380, cg2613
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
    Proteomesi
    • UP000000582 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemoved1 Publication
    Chaini2 – 328327Malate dehydrogenasePRO_0000113364Add
    BLAST

    2D gel databases

    World-2DPAGE0001:Q8NN33.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi196627.cg2613.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NN33.
    SMRiQ8NN33. Positions 8-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105D9Z. Bacteria.
    COG0039. LUCA.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiAFSQECI.
    OrthoDBiEOG6PP9Q2.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8NN33-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNSPQNVSTK KVTVTGAAGQ ISYSLLWRIA NGEVFGTDTP VELKLLEIPQ
    60 70 80 90 100
    ALGGAEGVAM ELLDSAFPLL RNITITADAN EAFDGANAAF LVGAKPRGKG
    110 120 130 140 150
    EERADLLANN GKIFGPQGKA INDNAADDIR VLVVGNPANT NALIASAAAP
    160 170 180 190 200
    DVPASRFNAM MRLDHNRAIS QLATKLGRGS AEFNNIVVWG NHSATQFPDI
    210 220 230 240 250
    TYATVGGEKV TDLVDHDWYV EEFIPRVANR GAEIIEVRGK SSAASAASSA
    260 270 280 290 300
    IDHMRDWVQG TEAWSSAAIP STGAYGIPEG IFVGLPTVSR NGEWEIVEGL
    310 320
    EISDFQRARI DANAQELQAE REAVRDLL
    Length:328
    Mass (Da):34,908
    Last modified:October 1, 2002 - v1
    Checksum:i1B725310F07A7EB0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti274 – 2741A → G in CAC83073 (Ref. 1) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ303072 Genomic DNA. Translation: CAC83073.1.
    BA000036 Genomic DNA. Translation: BAB99773.1.
    BX927155 Genomic DNA. Translation: CAF21045.1.
    RefSeqiNP_601581.1. NC_003450.3.
    WP_011015079.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99773; BAB99773; BAB99773.
    CAF21045; CAF21045; cg2613.
    GeneIDi1020330.
    KEGGicgb:cg2613.
    cgl:NCgl2297.
    PATRICi21496766. VBICorGlu203724_2315.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ303072 Genomic DNA. Translation: CAC83073.1.
    BA000036 Genomic DNA. Translation: BAB99773.1.
    BX927155 Genomic DNA. Translation: CAF21045.1.
    RefSeqiNP_601581.1. NC_003450.3.
    WP_011015079.1. NC_006958.1.

    3D structure databases

    ProteinModelPortaliQ8NN33.
    SMRiQ8NN33. Positions 8-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi196627.cg2613.

    2D gel databases

    World-2DPAGE0001:Q8NN33.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAB99773; BAB99773; BAB99773.
    CAF21045; CAF21045; cg2613.
    GeneIDi1020330.
    KEGGicgb:cg2613.
    cgl:NCgl2297.
    PATRICi21496766. VBICorGlu203724_2315.

    Phylogenomic databases

    eggNOGiENOG4105D9Z. Bacteria.
    COG0039. LUCA.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiAFSQECI.
    OrthoDBiEOG6PP9Q2.

    Enzyme and pathway databases

    BRENDAi1.1.1.37. 960.
    SABIO-RKQ8NN33.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning, sequencing and expression of the gene encoding malate dehydrogenase in the amino-acid producer Corynebacterium glutamicum."
      Kos P.B., Puskas L.G., Hackler L., Yukawa H.
      Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    4. "Purification and characterization of malate dehydrogenase from Corynebacterium glutamicum."
      Genda T., Nakamatsu T., Ozak H.
      J. Biosci. Bioeng. 95:562-566(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-9, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: ATCC 14067 / DSM 20411 / NCIB 9565 / 2247.

    Entry informationi

    Entry nameiMDH_CORGL
    AccessioniPrimary (citable) accession number: Q8NN33
    Secondary accession number(s): Q8VNW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: October 1, 2002
    Last modified: May 11, 2016
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.