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Q8NN33

- MDH_CORGL

UniProt

Q8NN33 - MDH_CORGL

Protein

Malate dehydrogenase

Gene

mdh

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei97 – 971SubstrateUniRule annotation
    Binding sitei103 – 1031SubstrateUniRule annotation
    Binding sitei110 – 1101NADUniRule annotation
    Binding sitei117 – 1171NADUniRule annotation
    Binding sitei136 – 1361SubstrateUniRule annotation
    Binding sitei167 – 1671SubstrateUniRule annotation
    Active sitei192 – 1921Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 227NADUniRule annotation
    Nucleotide bindingi134 – 1363NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi1.1.1.37. 1648.
    SABIO-RKQ8NN33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:Cgl2380, cg2613
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Malate dehydrogenasePRO_0000113364Add
    BLAST

    2D gel databases

    World-2DPAGE0001:Q8NN33.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196627.cg2613.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NN33.
    SMRiQ8NN33. Positions 8-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiAFSQECI.
    OrthoDBiEOG6PP9Q2.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8NN33-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSPQNVSTK KVTVTGAAGQ ISYSLLWRIA NGEVFGTDTP VELKLLEIPQ    50
    ALGGAEGVAM ELLDSAFPLL RNITITADAN EAFDGANAAF LVGAKPRGKG 100
    EERADLLANN GKIFGPQGKA INDNAADDIR VLVVGNPANT NALIASAAAP 150
    DVPASRFNAM MRLDHNRAIS QLATKLGRGS AEFNNIVVWG NHSATQFPDI 200
    TYATVGGEKV TDLVDHDWYV EEFIPRVANR GAEIIEVRGK SSAASAASSA 250
    IDHMRDWVQG TEAWSSAAIP STGAYGIPEG IFVGLPTVSR NGEWEIVEGL 300
    EISDFQRARI DANAQELQAE REAVRDLL 328
    Length:328
    Mass (Da):34,908
    Last modified:October 1, 2002 - v1
    Checksum:i1B725310F07A7EB0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti274 – 2741A → G in CAC83073. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ303072 Genomic DNA. Translation: CAC83073.1.
    BA000036 Genomic DNA. Translation: BAB99773.1.
    BX927155 Genomic DNA. Translation: CAF21045.1.
    RefSeqiNP_601581.1. NC_003450.3.
    YP_226625.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99773; BAB99773; BAB99773.
    CAF21045; CAF21045; cg2613.
    GeneIDi1020330.
    KEGGicgb:cg2613.
    cgl:NCgl2297.
    PATRICi21496766. VBICorGlu203724_2315.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ303072 Genomic DNA. Translation: CAC83073.1 .
    BA000036 Genomic DNA. Translation: BAB99773.1 .
    BX927155 Genomic DNA. Translation: CAF21045.1 .
    RefSeqi NP_601581.1. NC_003450.3.
    YP_226625.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali Q8NN33.
    SMRi Q8NN33. Positions 8-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg2613.

    2D gel databases

    World-2DPAGE 0001:Q8NN33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99773 ; BAB99773 ; BAB99773 .
    CAF21045 ; CAF21045 ; cg2613 .
    GeneIDi 1020330.
    KEGGi cgb:cg2613.
    cgl:NCgl2297.
    PATRICi 21496766. VBICorGlu203724_2315.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000220953.
    KOi K00024.
    OMAi AFSQECI.
    OrthoDBi EOG6PP9Q2.

    Enzyme and pathway databases

    BRENDAi 1.1.1.37. 1648.
    SABIO-RK Q8NN33.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01517. Malate_dehydrog_2.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and expression of the gene encoding malate dehydrogenase in the amino-acid producer Corynebacterium glutamicum."
      Kos P.B., Puskas L.G., Hackler L., Yukawa H.
      Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiMDH_CORGL
    AccessioniPrimary (citable) accession number: Q8NN33
    Secondary accession number(s): Q8VNW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3