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Q8NN33

- MDH_CORGL

UniProt

Q8NN33 - MDH_CORGL

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Protein

Malate dehydrogenase

Gene
mdh, Cgl2380, cg2613
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971Substrate By similarity
Binding sitei103 – 1031Substrate By similarity
Binding sitei110 – 1101NAD By similarity
Binding sitei117 – 1171NAD By similarity
Binding sitei136 – 1361Substrate By similarity
Binding sitei167 – 1671Substrate By similarity
Active sitei192 – 1921Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 227NAD By similarity
Nucleotide bindingi134 – 1363NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.37. 1648.
SABIO-RKQ8NN33.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:Cgl2380, cg2613
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Malate dehydrogenaseUniRule annotationPRO_0000113364Add
BLAST

2D gel databases

World-2DPAGE0001:Q8NN33.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg2613.

Structurei

3D structure databases

ProteinModelPortaliQ8NN33.
SMRiQ8NN33. Positions 8-327.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFSQECI.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NN33-1 [UniParc]FASTAAdd to Basket

« Hide

MNSPQNVSTK KVTVTGAAGQ ISYSLLWRIA NGEVFGTDTP VELKLLEIPQ    50
ALGGAEGVAM ELLDSAFPLL RNITITADAN EAFDGANAAF LVGAKPRGKG 100
EERADLLANN GKIFGPQGKA INDNAADDIR VLVVGNPANT NALIASAAAP 150
DVPASRFNAM MRLDHNRAIS QLATKLGRGS AEFNNIVVWG NHSATQFPDI 200
TYATVGGEKV TDLVDHDWYV EEFIPRVANR GAEIIEVRGK SSAASAASSA 250
IDHMRDWVQG TEAWSSAAIP STGAYGIPEG IFVGLPTVSR NGEWEIVEGL 300
EISDFQRARI DANAQELQAE REAVRDLL 328
Length:328
Mass (Da):34,908
Last modified:October 1, 2002 - v1
Checksum:i1B725310F07A7EB0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2741A → G in CAC83073. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ303072 Genomic DNA. Translation: CAC83073.1.
BA000036 Genomic DNA. Translation: BAB99773.1.
BX927155 Genomic DNA. Translation: CAF21045.1.
RefSeqiNP_601581.1. NC_003450.3.
YP_226625.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99773; BAB99773; BAB99773.
CAF21045; CAF21045; cg2613.
GeneIDi1020330.
KEGGicgb:cg2613.
cgl:NCgl2297.
PATRICi21496766. VBICorGlu203724_2315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ303072 Genomic DNA. Translation: CAC83073.1 .
BA000036 Genomic DNA. Translation: BAB99773.1 .
BX927155 Genomic DNA. Translation: CAF21045.1 .
RefSeqi NP_601581.1. NC_003450.3.
YP_226625.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q8NN33.
SMRi Q8NN33. Positions 8-327.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg2613.

2D gel databases

World-2DPAGE 0001:Q8NN33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99773 ; BAB99773 ; BAB99773 .
CAF21045 ; CAF21045 ; cg2613 .
GeneIDi 1020330.
KEGGi cgb:cg2613.
cgl:NCgl2297.
PATRICi 21496766. VBICorGlu203724_2315.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000220953.
KOi K00024.
OMAi AFSQECI.
OrthoDBi EOG6PP9Q2.

Enzyme and pathway databases

BRENDAi 1.1.1.37. 1648.
SABIO-RK Q8NN33.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and expression of the gene encoding malate dehydrogenase in the amino-acid producer Corynebacterium glutamicum."
    Kos P.B., Puskas L.G., Hackler L., Yukawa H.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiMDH_CORGL
AccessioniPrimary (citable) accession number: Q8NN33
Secondary accession number(s): Q8VNW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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