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Q8NN33 (MDH_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:Cgl2380, cg2613
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01517

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Malate dehydrogenase HAMAP-Rule MF_01517
PRO_0000113364

Regions

Nucleotide binding16 – 227NAD By similarity
Nucleotide binding134 – 1363NAD By similarity

Sites

Active site1921Proton acceptor By similarity
Binding site971Substrate By similarity
Binding site1031Substrate By similarity
Binding site1101NAD By similarity
Binding site1171NAD By similarity
Binding site1361Substrate By similarity
Binding site1671Substrate By similarity

Experimental info

Sequence conflict2741A → G in CAC83073. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8NN33 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 1B725310F07A7EB0

FASTA32834,908
        10         20         30         40         50         60 
MNSPQNVSTK KVTVTGAAGQ ISYSLLWRIA NGEVFGTDTP VELKLLEIPQ ALGGAEGVAM 

        70         80         90        100        110        120 
ELLDSAFPLL RNITITADAN EAFDGANAAF LVGAKPRGKG EERADLLANN GKIFGPQGKA 

       130        140        150        160        170        180 
INDNAADDIR VLVVGNPANT NALIASAAAP DVPASRFNAM MRLDHNRAIS QLATKLGRGS 

       190        200        210        220        230        240 
AEFNNIVVWG NHSATQFPDI TYATVGGEKV TDLVDHDWYV EEFIPRVANR GAEIIEVRGK 

       250        260        270        280        290        300 
SSAASAASSA IDHMRDWVQG TEAWSSAAIP STGAYGIPEG IFVGLPTVSR NGEWEIVEGL 

       310        320 
EISDFQRARI DANAQELQAE REAVRDLL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and expression of the gene encoding malate dehydrogenase in the amino-acid producer Corynebacterium glutamicum."
Kos P.B., Puskas L.G., Hackler L., Yukawa H.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
[2]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ303072 Genomic DNA. Translation: CAC83073.1.
BA000036 Genomic DNA. Translation: BAB99773.1.
BX927155 Genomic DNA. Translation: CAF21045.1.
RefSeqNP_601581.1. NC_003450.3.
YP_226625.1. NC_006958.1.

3D structure databases

ProteinModelPortalQ8NN33.
SMRQ8NN33. Positions 8-327.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg2613.

2D gel databases

World-2DPAGE0001:Q8NN33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB99773; BAB99773; BAB99773.
CAF21045; CAF21045; cg2613.
GeneID1020330.
3345438.
KEGGcgb:cg2613.
cgl:NCgl2297.
PATRIC21496766. VBICorGlu203724_2315.

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000220953.
KOK00024.
OMACRMLPSG.
OrthoDBEOG6PP9Q2.
ProtClustDBPRK05442.

Enzyme and pathway databases

BRENDA1.1.1.37. 1648.
SABIO-RKQ8NN33.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01517. Malate_dehydrog_2.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_CORGL
AccessionPrimary (citable) accession number: Q8NN33
Secondary accession number(s): Q8VNW2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families