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Protein

Malate dehydrogenase

Gene

mdh

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971SubstrateUniRule annotation
Binding sitei103 – 1031SubstrateUniRule annotation
Binding sitei110 – 1101NADUniRule annotation
Binding sitei117 – 1171NADUniRule annotation
Binding sitei136 – 1361SubstrateUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation
Active sitei192 – 1921Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 227NADUniRule annotation
Nucleotide bindingi134 – 1363NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.37. 960.
SABIO-RKQ8NN33.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Cgl2380, cg2613
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Malate dehydrogenasePRO_0000113364Add
BLAST

2D gel databases

World-2DPAGE0001:Q8NN33.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg2613.

Structurei

3D structure databases

ProteinModelPortaliQ8NN33.
SMRiQ8NN33. Positions 8-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFKDTDY.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NN33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSPQNVSTK KVTVTGAAGQ ISYSLLWRIA NGEVFGTDTP VELKLLEIPQ
60 70 80 90 100
ALGGAEGVAM ELLDSAFPLL RNITITADAN EAFDGANAAF LVGAKPRGKG
110 120 130 140 150
EERADLLANN GKIFGPQGKA INDNAADDIR VLVVGNPANT NALIASAAAP
160 170 180 190 200
DVPASRFNAM MRLDHNRAIS QLATKLGRGS AEFNNIVVWG NHSATQFPDI
210 220 230 240 250
TYATVGGEKV TDLVDHDWYV EEFIPRVANR GAEIIEVRGK SSAASAASSA
260 270 280 290 300
IDHMRDWVQG TEAWSSAAIP STGAYGIPEG IFVGLPTVSR NGEWEIVEGL
310 320
EISDFQRARI DANAQELQAE REAVRDLL
Length:328
Mass (Da):34,908
Last modified:October 1, 2002 - v1
Checksum:i1B725310F07A7EB0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2741A → G in CAC83073 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303072 Genomic DNA. Translation: CAC83073.1.
BA000036 Genomic DNA. Translation: BAB99773.1.
BX927155 Genomic DNA. Translation: CAF21045.1.
RefSeqiNP_601581.1. NC_003450.3.
WP_011015079.1. NC_006958.1.
YP_226625.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99773; BAB99773; BAB99773.
CAF21045; CAF21045; cg2613.
GeneIDi1020330.
KEGGicgb:cg2613.
cgl:NCgl2297.
PATRICi21496766. VBICorGlu203724_2315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303072 Genomic DNA. Translation: CAC83073.1.
BA000036 Genomic DNA. Translation: BAB99773.1.
BX927155 Genomic DNA. Translation: CAF21045.1.
RefSeqiNP_601581.1. NC_003450.3.
WP_011015079.1. NC_006958.1.
YP_226625.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NN33.
SMRiQ8NN33. Positions 8-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg2613.

2D gel databases

World-2DPAGE0001:Q8NN33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99773; BAB99773; BAB99773.
CAF21045; CAF21045; cg2613.
GeneIDi1020330.
KEGGicgb:cg2613.
cgl:NCgl2297.
PATRICi21496766. VBICorGlu203724_2315.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFKDTDY.
OrthoDBiEOG6PP9Q2.

Enzyme and pathway databases

BRENDAi1.1.1.37. 960.
SABIO-RKQ8NN33.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and expression of the gene encoding malate dehydrogenase in the amino-acid producer Corynebacterium glutamicum."
    Kos P.B., Puskas L.G., Hackler L., Yukawa H.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiMDH_CORGL
AccessioniPrimary (citable) accession number: Q8NN33
Secondary accession number(s): Q8VNW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2002
Last modified: June 24, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.