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Q8NN33

- MDH_CORGL

UniProt

Q8NN33 - MDH_CORGL

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Protein

Malate dehydrogenase

Gene

mdh

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971SubstrateUniRule annotation
Binding sitei103 – 1031SubstrateUniRule annotation
Binding sitei110 – 1101NADUniRule annotation
Binding sitei117 – 1171NADUniRule annotation
Binding sitei136 – 1361SubstrateUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation
Active sitei192 – 1921Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 227NADUniRule annotation
Nucleotide bindingi134 – 1363NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.37. 1648.
SABIO-RKQ8NN33.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Cgl2380, cg2613
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Malate dehydrogenasePRO_0000113364Add
BLAST

2D gel databases

World-2DPAGE0001:Q8NN33.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg2613.

Structurei

3D structure databases

ProteinModelPortaliQ8NN33.
SMRiQ8NN33. Positions 8-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFSQECI.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NN33-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSPQNVSTK KVTVTGAAGQ ISYSLLWRIA NGEVFGTDTP VELKLLEIPQ
60 70 80 90 100
ALGGAEGVAM ELLDSAFPLL RNITITADAN EAFDGANAAF LVGAKPRGKG
110 120 130 140 150
EERADLLANN GKIFGPQGKA INDNAADDIR VLVVGNPANT NALIASAAAP
160 170 180 190 200
DVPASRFNAM MRLDHNRAIS QLATKLGRGS AEFNNIVVWG NHSATQFPDI
210 220 230 240 250
TYATVGGEKV TDLVDHDWYV EEFIPRVANR GAEIIEVRGK SSAASAASSA
260 270 280 290 300
IDHMRDWVQG TEAWSSAAIP STGAYGIPEG IFVGLPTVSR NGEWEIVEGL
310 320
EISDFQRARI DANAQELQAE REAVRDLL
Length:328
Mass (Da):34,908
Last modified:October 1, 2002 - v1
Checksum:i1B725310F07A7EB0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2741A → G in CAC83073. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303072 Genomic DNA. Translation: CAC83073.1.
BA000036 Genomic DNA. Translation: BAB99773.1.
BX927155 Genomic DNA. Translation: CAF21045.1.
RefSeqiNP_601581.1. NC_003450.3.
YP_226625.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99773; BAB99773; BAB99773.
CAF21045; CAF21045; cg2613.
GeneIDi1020330.
3345438.
KEGGicgl:NCgl2297.
PATRICi21496766. VBICorGlu203724_2315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303072 Genomic DNA. Translation: CAC83073.1 .
BA000036 Genomic DNA. Translation: BAB99773.1 .
BX927155 Genomic DNA. Translation: CAF21045.1 .
RefSeqi NP_601581.1. NC_003450.3.
YP_226625.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q8NN33.
SMRi Q8NN33. Positions 8-327.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg2613.

2D gel databases

World-2DPAGE 0001:Q8NN33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99773 ; BAB99773 ; BAB99773 .
CAF21045 ; CAF21045 ; cg2613 .
GeneIDi 1020330.
3345438.
KEGGi cgl:NCgl2297.
PATRICi 21496766. VBICorGlu203724_2315.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000220953.
KOi K00024.
OMAi AFSQECI.
OrthoDBi EOG6PP9Q2.

Enzyme and pathway databases

BRENDAi 1.1.1.37. 1648.
SABIO-RK Q8NN33.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and expression of the gene encoding malate dehydrogenase in the amino-acid producer Corynebacterium glutamicum."
    Kos P.B., Puskas L.G., Hackler L., Yukawa H.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiMDH_CORGL
AccessioniPrimary (citable) accession number: Q8NN33
Secondary accession number(s): Q8VNW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3