Q8NMT3 (GLSA_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase
EC=3.5.1.2

Gene names

Name:	glsA
Ordered Locus Names:	Cgl2482, cg2728

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family. Contains 1 STAS domain.
Sequence caution	The sequence BAB99875.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Glutaminase HAMAP-Rule MF_00313		PRO_0000110606
Regions
	Domain	316 – 413	98	STAS
	Region	23 – 307	285	Glutaminase HAMAP-Rule MF_00313
Sites
	Binding site	65	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	261	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NMT3 [UniParc]. Last modified May 24, 2004. Version 2. Checksum: 91ADD397B9FFE473	FASTA	413	44,748
	10 20 30 40 50 60 MLTMPIPEYL HEILDDVRDT TSGELADYIP ELKSADPNPL AVALCTVNGH IYSAGDDDIE 70 80 90 100 110 120 FTMQSISKPF AYALALQECG FDEVSASVAL EPSGEAFNEL SLDGENRPMN PMINAGAIAI 130 140 150 160 170 180 NQLINGSDST VEDRVEKIRH YFSELAGREL TIDRVLAESE LAGADRNLSI AHMLRNYGVI 190 200 210 220 230 240 EDEAHDAVLS YTLQCAIKVT TRDLAVMTAT LAAGGTHPIT GKKLLDARVC RLTLSVMASA 250 260 270 280 290 300 GMYDEAGQWL STVGIPAKSG VAGGLIGILP GQLGIATFSP RLNPKGNSVR GVKIFKQLSD 310 320 330 340 350 360 DMGLHLMSTE QVSGHAVRSI TRDGDTTFIQ MQGAMNFSAS ESFLHAIVEH NFEGTEVVLD 370 380 390 400 410 LTRVLSFHPV AIRMIKEGLK RIRDAGFEVF ILDPDDVLPD FMFSDGTICK ERV

References

[1]

"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99875.1. Different initiation. BX927155 Genomic DNA. Translation: CAF21143.1.
RefSeq	NP_601682.1. NC_003450.3. YP_226722.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NMT3.
SMR	Q8NMT3. Positions 3-349.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg2728.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB99875; BAB99875; BAB99875. CAF21143; CAF21143; cg2728.
GeneID	1020428. 3342801.
KEGG	cgb:cg2728. cgl:NCgl2395.
PATRIC	21496976. VBICorGlu203724_2414.
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	VYTKQCA.
ProtClustDB	PRK00971.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-2460-MONOMER.
Family and domain databases
Gene3D	3.30.750.24. 1 hit. 3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase. Fused.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. IPR002645. STAS_dom. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit. SSF52091. STAS. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
PROSITE	PS50801. STAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLSA_CORGL

Accession

Primary (citable) accession number: Q8NMT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program