Q8NMR5 (NTPA_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

Cgl2500, cg2751

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178159
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	72 – 73	2	Substrate binding By similarity
Sites
	Metal binding	72	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	188	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NMR5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 90D87C1F9301A2C3	FASTA	206	22,098
	10 20 30 40 50 60 MKLLLASNNA KKLKELQRIL DQAGLDSVEL LALRDVEAYD EPIEDGRTFA DNAQIKARAG 70 80 90 100 110 120 VTHTGIATIA DDSGIAVEEL NGMPGVLSAR WSGAHGNDTA NNELLLAQME HVPDERRNAA 130 140 150 160 170 180 FVSVCVLALP DGQEFVQEGR WEGQLLRGPK GENGFGYDPL FIPAEEIDGQ GRSSAELSAE 190 200 EKDALSHRGQ ALRGLVEKIA QVAAAS

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99893.1. BX927155 Genomic DNA. Translation: CAF21163.1.
RefSeq	NP_601702.1. NC_003450.3. YP_226742.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NMR5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020447. 3342886.
GenomeReviews	Gene locus Cgl2500 in contig BA000036_GR. Gene locus cg2751 in contig BX927147_GR.
KEGG	cgb:cg2751. cgl:NCgl2414.
PATRIC	21497016. VBICorGlu203724_2433.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
PhylomeDB	Q8NMR5.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2751-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_CORGL

Accession

Primary (citable) accession number: Q8NMR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program