ID   NADE_CORGL              Reviewed;         277 AA.
AC   Q8NMN7;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 39.
DE   RecName: Full=NH(3)-dependent NAD(+) synthetase;
DE            EC=6.3.1.5;
GN   Name=nadE; OrderedLocusNames=Cgl2534, cg2792;
OS   Corynebacterium glutamicum (Brevibacterium flavum).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Corynebacteriaceae; Corynebacterium.
OX   NCBI_TaxID=1718;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
RA   Nakagawa S.;
RT   "Complete genomic sequence of Corynebacterium glutamicum ATCC 13032.";
RL   Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
RX   MEDLINE=22830012; PubMed=12948626; DOI=10.1016/S0168-1656(03)00154-8;
RA   Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M.,
RA   Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L.,
RA   Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B.,
RA   McHardy A.C., Meyer F., Moeckel B., Pfefferle W., Puehler A.,
RA   Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I.,
RA   Tauch A.;
RT   "The complete Corynebacterium glutamicum ATCC 13032 genome sequence
RT   and its impact on the production of L-aspartate-derived amino acids
RT   and vitamins.";
RL   J. Biotechnol. 104:5-25(2003).
CC   -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP +
CC       diphosphate + NAD(+).
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (ammonia route): step 1/1.
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
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DR   EMBL; BA000036; BAB99927.1; -; Genomic_DNA.
DR   EMBL; BX927155; CAF21196.1; -; Genomic_DNA.
DR   RefSeq; NP_601734.1; -.
DR   RefSeq; YP_226775.1; -.
DR   HSSP; P08164; 1IH8.
DR   World-2DPAGE; 0001:Q8NMN7; -.
DR   PRIDE; Q8NMN7; -.
DR   GeneID; 1020481; -.
DR   GeneID; 3343843; -.
DR   GenomeReviews; BA000036_GR; Cgl2534.
DR   GenomeReviews; BX927147_GR; cg2792.
DR   KEGG; cgb:cg2792; -.
DR   KEGG; cgl:NCgl2446; -.
DR   HOGENOM; Q8NMN7; -.
DR   OMA; Q8NMN7; ADLEEDR.
DR   BioCyc; CGLU196627-1:CG2792-MON; -.
DR   BRENDA; 6.3.1.5; 812.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0008795; F:NAD+ synthase activity; IEA:EC.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00193; -; 1.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding.
FT   CHAIN         1    277       NH(3)-dependent NAD(+) synthetase.
FT                                /FTId=PRO_0000152165.
FT   NP_BIND      46     53       ATP (By similarity).
FT   ACT_SITE     48     48       By similarity.
SQ   SEQUENCE   277 AA;  30426 MW;  18C1F62CEA756E35 CRC64;
     MTNTQTEIIN ELKVSPAIDV AKEVEFRVQF LVDYLRASHT KGFVLGISGG QDSTLAGRLT
     QLAVERIRAE ENSTDYVFYA VRLPYAIQAD EDDAQVALEF IAPDKSVTVN VKDATDATEA
     TVAAALELPE LTDFNRGNIK ARQRMVAQYA IAGQLGLLVI GTDHAAENVT GFFTKFGDGA
     ADLLPLAGLS KRQGAAILEH LGAPSSTWTK VPTADLEEDR PALPDEEALG VSYADIDNYL
     ENKPDVSEKA QQRIEHLWKV GQHKRHLPAT PQENWWR
//