Q8NMN7 (NADE_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Cgl2534, cg2792

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152165
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NMN7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 18C1F62CEA756E35	FASTA	277	30,426
	10 20 30 40 50 60 MTNTQTEIIN ELKVSPAIDV AKEVEFRVQF LVDYLRASHT KGFVLGISGG QDSTLAGRLT 70 80 90 100 110 120 QLAVERIRAE ENSTDYVFYA VRLPYAIQAD EDDAQVALEF IAPDKSVTVN VKDATDATEA 130 140 150 160 170 180 TVAAALELPE LTDFNRGNIK ARQRMVAQYA IAGQLGLLVI GTDHAAENVT GFFTKFGDGA 190 200 210 220 230 240 ADLLPLAGLS KRQGAAILEH LGAPSSTWTK VPTADLEEDR PALPDEEALG VSYADIDNYL 250 260 270 ENKPDVSEKA QQRIEHLWKV GQHKRHLPAT PQENWWR

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99927.1. BX927155 Genomic DNA. Translation: CAF21196.1.
RefSeq	NP_601734.1. NC_003450.3. YP_226775.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NMN7.
SMR	Q8NMN7. Positions 4-277.
ModBase	Search...
2D gel databases
World-2DPAGE	0001:Q8NMN7.
Proteomic databases
PRIDE	Q8NMN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020481. 3343843.
GenomeReviews	Gene locus Cgl2534 in contig BA000036_GR. Gene locus cg2792 in contig BX927147_GR.
KEGG	cgb:cg2792. cgl:NCgl2446.
PATRIC	21497084. VBICorGlu203724_2467.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	GLNKRRV.
PhylomeDB	Q8NMN7.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2792-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CORGL

Accession

Primary (citable) accession number: Q8NMN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program