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Q8NMI9 (Q8NMI9_CORGL) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Name:purF EMBL CAF21246.1
Ordered Locus Names:cg2857, Cgl2584
OrganismCorynebacterium glutamicum (Brevibacterium flavum)
Taxonomic identifier1718 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site201For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2591Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4051Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4631Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4661Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
Q8NMI9 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 9C1FC286CAEFEBAE

FASTA49452,876
        10         20         30         40         50         60 
MTQVNHPYDD LNEQAPQEEC GVFGVWAPGE EVSKLTYFGL FALQHRGQEA AGIAVGDGEQ 

        70         80         90        100        110        120 
ILVFKDLGLV SQVFDEPILE SLRGNIAIGH TRYTTAGGNT WENAQPMFRM APDGTDIALG 

       130        140        150        160        170        180 
HNGNLINYIE LLDKATELGL VDPAKKPSDT DVLTGLLASG VHDGNNLFDS AKELLPSVKG 

       190        200        210        220        230        240 
AYCLTFTDGH TLYAARDPFG IRPLSIGRLE RGWVVASETA ALDIVGASHV REVEPGELIA 

       250        260        270        280        290        300 
IDESGLKSAR FAETTRKGCV FEYVYLARPD SVIKGRNVNE ARLEIGRKLA AEAPAVGDLV 

       310        320        330        340        350        360 
IPTPESGTPA AVGFAQASGI PFGQGMVKNA YVGRTFIQPS DTLRQLGIRL KLNPLREVIA 

       370        380        390        400        410        420 
GKRLVVVDDS IVRGNTQRAV IRMLREAGAA EVHVRIASPP VKWPCFYGID FATPGELIAN 

       430        440        450        460        470        480 
AVTSDNEAEM VEAVRSAIGA DTLGYVSIDS MVAATEQPAN ELCIACFDGK YPMGLPQGNS 

       490 
NADLVRKMQA TASS

« Hide

References

[1]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025 [Kalinowski].
[2]"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032."
Nakagawa S.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025 [Nakagawa].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000036 Genomic DNA. Translation: BAB99977.1.
BX927155 Genomic DNA. Translation: CAF21246.1.
RefSeqNP_601782.1. NC_003450.3.
YP_226825.1. NC_006958.1.

3D structure databases

HSSPHSSP built from PDB template 1AO0 based on UniProtKB P00497.
ProteinModelPortalQ8NMI9.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1020530.
3345335.
GenomeReviewsGene locus Cgl2584 in contig BA000036_GR.
Gene locus cg2857 in contig BX927147_GR.
KEGGcgb:cg2857.
cgl:NCgl2495.
PATRIC21497210. VBICorGlu203724_2518.

Phylogenomic databases

HOGENOMHBG392416.
OMAVWAPGEE.
PhylomeDBQ8NMI9.
ProtClustDBPRK07847.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ8NMI9_CORGL
AccessionPrimary (citable) accession number: Q8NMI9
Secondary accession number(s): Q6M2P8
Entry history
Integrated into UniProtKB/TrEMBL: October 1, 2002
Last sequence update: October 1, 2002
Last modified: January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info