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Q8NMH3 (PUR2_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Cgl2600, cg2878
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151446

Regions

Domain108 – 311204ATP-grasp
Nucleotide binding137 – 19256ATP By similarity

Sites

Metal binding2811Magnesium or manganese By similarity
Metal binding2831Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8NMH3 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7C9700A1F83EAAFD

FASTA42143,545
        10         20         30         40         50         60 
MRILVIGSGA REHALLRGLS TDPATTELHV APGNAGLGSI ATVHPGIKAD DPEAVTALAK 

        70         80         90        100        110        120 
ELNSDLVVIG PEIPLVAGVA DALRAAGIAV FGPNKDAARI EGSKAFAKDV MAAQGVRTAH 

       130        140        150        160        170        180 
AEAITPGASS EDIDAAIDRF GPTWVVKDDG LAAGKGVVVT PDRAAARAHV DAVLEGGNPV 

       190        200        210        220        230        240 
LLESFLDGPE VSLFCLVDGE TVVPLLPAQD HKRAYDNDEG PNTGGMGAYA PLPWLPEDGV 

       250        260        270        280        290        300 
QRIVDEVCVP VAREMVARGC AYSGLLYAGI AWGAEGPAVV EFNCRFGDPE TQAVLALLKT 

       310        320        330        340        350        360 
PLAVLLNAVA TGTLAEQPAL EWEDAYALTV VLASYNYPEA PRTGDVIRNA DADNVLHAGT 

       370        380        390        400        410        420 
ALNAEGELVS AGGRVLNVIG VGETLEAARD NAYTTIKDIE LEGSHYRSDI ALAALEGRIS 


I 

« Hide

References

[1]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000036 Genomic DNA. Translation: BAB99993.1.
BX927155 Genomic DNA. Translation: CAF21262.1.
RefSeqNP_601800.1. NC_003450.3.
YP_226841.1. NC_006958.1.

3D structure databases

ProteinModelPortalQ8NMH3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg2878.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB99993; BAB99993; BAB99993.
CAF21262; CAF21262; cg2878.
GeneID1020546.
3343136.
KEGGcgb:cg2878.
cgl:NCgl2511.
PATRIC21497242. VBICorGlu203724_2534.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_CORGL
AccessionPrimary (citable) accession number: Q8NMH3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways