Q8NMH3 (PUR2_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Cgl2600, cg2878

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151446
Regions
	Domain	108 – 311	204	ATP-grasp
	Nucleotide binding	137 – 192	56	ATP By similarity
Sites
	Metal binding	281	1	Magnesium or manganese By similarity
	Metal binding	283	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NMH3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 7C9700A1F83EAAFD	FASTA	421	43,545
	10 20 30 40 50 60 MRILVIGSGA REHALLRGLS TDPATTELHV APGNAGLGSI ATVHPGIKAD DPEAVTALAK 70 80 90 100 110 120 ELNSDLVVIG PEIPLVAGVA DALRAAGIAV FGPNKDAARI EGSKAFAKDV MAAQGVRTAH 130 140 150 160 170 180 AEAITPGASS EDIDAAIDRF GPTWVVKDDG LAAGKGVVVT PDRAAARAHV DAVLEGGNPV 190 200 210 220 230 240 LLESFLDGPE VSLFCLVDGE TVVPLLPAQD HKRAYDNDEG PNTGGMGAYA PLPWLPEDGV 250 260 270 280 290 300 QRIVDEVCVP VAREMVARGC AYSGLLYAGI AWGAEGPAVV EFNCRFGDPE TQAVLALLKT 310 320 330 340 350 360 PLAVLLNAVA TGTLAEQPAL EWEDAYALTV VLASYNYPEA PRTGDVIRNA DADNVLHAGT 370 380 390 400 410 420 ALNAEGELVS AGGRVLNVIG VGETLEAARD NAYTTIKDIE LEGSHYRSDI ALAALEGRIS I

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99993.1. BX927155 Genomic DNA. Translation: CAF21262.1.
RefSeq	NP_601800.1. NC_003450.3. YP_226841.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NMH3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020546. 3343136.
GenomeReviews	Gene locus Cgl2600 in contig BA000036_GR. Gene locus cg2878 in contig BX927147_GR.
KEGG	cgb:cg2878. cgl:NCgl2511.
PATRIC	21497242. VBICorGlu203724_2534.
Phylogenomic databases
HOGENOM	HBG404060.
OMA	MGAYTPL.
PhylomeDB	Q8NMH3.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2878-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01945.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. PurD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_CORGL

Accession

Primary (citable) accession number: Q8NMH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program