Q8NMD7 (SYC_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Synonyms:	cysS1
Ordered Locus Names:	Cgl2641, cg2924

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159388
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	212	1	Zinc By similarity
	Metal binding	237	1	Zinc By similarity
	Metal binding	241	1	Zinc By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NMD7 [UniParc]. Last modified October 25, 2002. Version 1. Checksum: C28B536BFC24C44A	FASTA	460	51,047
	10 20 30 40 50 60 MTLRIFDTGT RTLRDFKPVQ PGHASVYLCG ATPQSSPHIG HVRSAVAFDI LRRWLMAKGL 70 80 90 100 110 120 DVAFVRNVTD IDDKILTKAS ENGRPWWEWV STYEREFTWT YNTLGVLPPS TEPRATGHVT 130 140 150 160 170 180 QMIKYMQRLI DNGFAYAVDG SVYFDVAAWS KAEGSDYGSL SGNRVEDMEQ GEPDNFGKRG 190 200 210 220 230 240 PQDFALWKAA KPGEPSWPTP WGDGRPGWHL ECSAMATYYL GEQFDIHCGG LDLQFPHHEN 250 260 270 280 290 300 EIAQAHAAGD KFANYWMHNH WVTMAGEKMS KSLGNVLAVP EMLKQVRPVE LRYYLGSAHY 310 320 330 340 350 360 RSVLEYSESA LSEAAVGYRR IESFLERVGD VEVGEWTPGF EVAMDEDIAV PKALAEIHNA 370 380 390 400 410 420 VREGNAALDK GDREAAEKLA SSVRAMTGVL GFDPVEWGSD AGADGKADKA LDVLISSELE 430 440 450 460 RRATARAEKN WAVADEVRDR LADAGIEVVD TADGATWKLQ

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAC00035.1. BX927155 Genomic DNA. Translation: CAF21303.1.
RefSeq	NP_601841.1. NC_003450.3. YP_226882.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NMD7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020589. 3342707.
GenomeReviews	Gene locus Cgl2641 in contig BA000036_GR. Gene locus cg2924 in contig BX927147_GR.
KEGG	cgb:cg2924. cgl:NCgl2552.
PATRIC	21497330. VBICorGlu203724_2577.
Phylogenomic databases
HOGENOM	HBG327651.
OMA	DFDALNM.
PhylomeDB	Q8NMD7.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2924-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_CORGL

Accession

Primary (citable) accession number: Q8NMD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program