Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei995-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1005-phosphoribose 1-diphosphateUniRule annotation1
Binding sitei1035-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1055-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei129OrotateUniRule annotation1
Binding sitei157OrotateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciCORYNE:G18NG-12390-MONOMER.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:Cgl2773, cg3071
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001106911 – 184Orotate phosphoribosyltransferaseAdd BLAST184

Proteomic databases

PRIDEiQ8NM11.

2D gel databases

World-2DPAGE0001:Q8NM11.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg3071.

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 2110
Helixi43 – 464
Helixi49 – 6113
Turni62 – 654
Beta strandi69 – 746
Turni75 – 773
Helixi78 – 8710
Beta strandi88 – 914
Beta strandi94 – 985
Beta strandi110 – 1145
Beta strandi120 – 1278
Beta strandi129 – 1313
Helixi132 – 14413
Beta strandi147 – 1559
Helixi161 – 1666
Turni167 – 1693
Beta strandi172 – 1765
Turni179 – 1824

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P1ZX-ray2.44A/B12-173[»]
ProteinModelPortaliQ8NM11.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NM11.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 1335-phosphoribose 1-diphosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
KOiK00762.
OMAiYVNCKPV.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NM11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNSINAEA RAELAELIKE LAVVHGEVTL SSGKKADYYI DVRRATLHAR
60 70 80 90 100
ASRLIGQLLR EATADWDYDA VGGLTLGADP VATAIMHADG RDINAFVVRK
110 120 130 140 150
EAKKHGMQRR IEGPDLTGKK VLVVEDTTTT GNSPLTAVAA LREAGIEVVG
160 170 180
VATVVDRATG ADEVIAAEGL PYRSLLGLSD LGLN
Length:184
Mass (Da):19,445
Last modified:October 10, 2002 - v1
Checksum:iBBD7CF256BAB93B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAC00167.1.
BX927156 Genomic DNA. Translation: CAF20794.1.
RefSeqiNP_601967.1. NC_003450.3.
WP_003862850.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAC00167; BAC00167; BAC00167.
CAF20794; CAF20794; cg3071.
GeneIDi1020717.
KEGGicgb:cg3071.
cgl:NCgl2676.
PATRICi21497584. VBICorGlu203724_2704.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAC00167.1.
BX927156 Genomic DNA. Translation: CAF20794.1.
RefSeqiNP_601967.1. NC_003450.3.
WP_003862850.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P1ZX-ray2.44A/B12-173[»]
ProteinModelPortaliQ8NM11.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg3071.

2D gel databases

World-2DPAGE0001:Q8NM11.

Proteomic databases

PRIDEiQ8NM11.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC00167; BAC00167; BAC00167.
CAF20794; CAF20794; cg3071.
GeneIDi1020717.
KEGGicgb:cg3071.
cgl:NCgl2676.
PATRICi21497584. VBICorGlu203724_2704.

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
KOiK00762.
OMAiYVNCKPV.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
BioCyciCORYNE:G18NG-12390-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8NM11.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRE_CORGL
AccessioniPrimary (citable) accession number: Q8NM11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.