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Q8NLS3

- TRMB_CORGL

UniProt

Q8NLS3 - TRMB_CORGL

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei83 – 831S-adenosyl-L-methionineUniRule annotation
    Binding sitei108 – 1081S-adenosyl-L-methionineUniRule annotation
    Binding sitei135 – 1351S-adenosyl-L-methionineUniRule annotation
    Active sitei158 – 1581By similarity
    Binding sitei158 – 1581S-adenosyl-L-methionineUniRule annotation
    Binding sitei162 – 1621SubstrateUniRule annotation
    Binding sitei194 – 1941SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:Cgl2865, cg3172
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 255255tRNA (guanine-N(7)-)-methyltransferasePRO_0000171324Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi196627.cg3172.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NLS3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni164 – 1696Interaction with RNAUniRule annotation
    Regioni232 – 2354Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiLHIATDH.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8NLS3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSISDNSRDQ LGELPAGRPL QSDFDNDLDY PRLGSVTFRR GTLTENQQTM    50
    WDEKWPELGR VLEDELIDVD AWFGREGAKT IVEIGSGTGT STAAMAPLEA 100
    DTNIVAVELY KPGLAKLMGS VVRGEIDNVR MVRGDGIEVL NRMFADGSLD 150
    GIRVYFPDPW PKARHNKRRI IQSGPLNLFA KKLKPGGVLH VATDHADYAE 200
    WINELVEVEP LLEYKGWPWE ECPQLTDRQV ITKFEGKGLE KDHVINEYLW 250
    QKVQN 255
    Length:255
    Mass (Da):28,806
    Last modified:October 1, 2002 - v1
    Checksum:i550F5003FAE01AA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000036 Genomic DNA. Translation: BAC00259.1.
    BX927156 Genomic DNA. Translation: CAF20890.1.
    RefSeqiNP_602057.1. NC_003450.3.
    YP_227106.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAC00259; BAC00259; BAC00259.
    CAF20890; CAF20890; cg3172.
    GeneIDi1020808.
    KEGGicgb:cg3172.
    cgl:NCgl2767.
    PATRICi21497772. VBICorGlu203724_2797.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000036 Genomic DNA. Translation: BAC00259.1 .
    BX927156 Genomic DNA. Translation: CAF20890.1 .
    RefSeqi NP_602057.1. NC_003450.3.
    YP_227106.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali Q8NLS3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg3172.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC00259 ; BAC00259 ; BAC00259 .
    CAF20890 ; CAF20890 ; cg3172 .
    GeneIDi 1020808.
    KEGGi cgb:cg3172.
    cgl:NCgl2767.
    PATRICi 21497772. VBICorGlu203724_2797.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi LHIATDH.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiTRMB_CORGL
    AccessioniPrimary (citable) accession number: Q8NLS3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3