Q8NLP9 (GLPK_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Cgl2890, cg3198

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Glycerol kinase HAMAP MF_00186		PRO_0000059448
Regions
	Nucleotide binding	423 – 427	5	ATP By similarity
Sites
	Binding site	17	1	Substrate By similarity
	Binding site	21	1	ATP By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	256	1	Substrate By similarity
	Binding site	278	1	ATP By similarity
	Binding site	322	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NLP9 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: EC8B65618D84C480	FASTA	509	56,145
	10 20 30 40 50 60 MRISKANAYV AAIDQGTTST RCIFIDAQGK VVSSASKEHR QIFPQQGWVE HDPEEIWDNI 70 80 90 100 110 120 RSVVSQAMVS IDITPHEVAS LGVTNQRETT VVWDKHTGEP VYNAIVWQDT RTSDICLEIA 130 140 150 160 170 180 GEEGQEKWLD RTGLLINSYP SGPKIKWILD NVEGARERAE KGDLLFGTMD TWVLWNLTGG 190 200 210 220 230 240 VRGDDGDDAI HVTDVTNASR TLLMDLRTQQ WDPELCEALD IPMSMLPEIR PSVGEFRSVR 250 260 270 280 290 300 HRGTLADVPI TGVLGDQQAA LFGQGGFHEG AAKNTYGTGL FLLMNTGTSL KISEHGLLST 310 320 330 340 350 360 IAYQREGSAP VYALEGSVSM GGSLVQWLRD NLQLIPNAPA IENLAREVED NGGVHVVPAF 370 380 390 400 410 420 TGLFAPRWRP DARGVITGLT RFANRKHIAR AVLEANAFQT REVVDAMAKD AGKALESLRV 430 440 450 460 470 480 DGAMVENDLL MQMQADFLGI DVQRLEDVET TAVGVAFAAG LGSGFFKTTD EIEKLIAVKK 490 500 VWNPDMSEEE RERRYAEWNR AVEHSYDQA

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAC00284.1. BX927156 Genomic DNA. Translation: CAF20913.1.
RefSeq	NP_602080.1. NC_003450.3. YP_227129.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NLP9.
SMR	Q8NLP9. Positions 8-506.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1020833. 3345184.
GenomeReviews	Gene locus Cgl2890 in contig BA000036_GR. Gene locus cg3198 in contig BX927147_GR.
KEGG	cgb:cg3198. cgl:NCgl2790.
PATRIC	21497820. VBICorGlu203724_2821.
Phylogenomic databases
HOGENOM	HBG511469.
OMA	ATYFSGT.
PhylomeDB	Q8NLP9.
ProtClustDB	PRK00047.
Enzyme and pathway databases
BioCyc	CGLU196627:CG3198-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_CORGL

Accession

Primary (citable) accession number: Q8NLP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program