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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei725 – 7251ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-2998-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Cgl3018, cg3346
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 952952Leucine--tRNA ligasePRO_0000152007Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196627.NCgl2915.

Structurei

3D structure databases

ProteinModelPortaliQ8NLC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7712"HIGH" regionAdd
BLAST
Motifi722 – 7265"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NLC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNPSEGTTP LAFRYTPELA NKIEGEWQNY WTDNGTFNAP NPVGDLAPAD
60 70 80 90 100
GKALPEDKLF VQDMFPYPSG AGLHVGHPLG YIATDVFARY NRMLGKNVLH
110 120 130 140 150
TLGYDAFGLP AEQYAIQTGT HPRTTTMANI ENMKRQLGAL GLGHDSRRAV
160 170 180 190 200
ATTDPEFYKW TQWIFLQIFN SWFDAEQQKA RPISELIPLL ESGELKTKDG
210 220 230 240 250
ADYNALGDVE KQKAVDDYRL VYRSNSTVNW CPGLGTVLAN EEVTADGRSE
260 270 280 290 300
RGNFPVFRKN LSQWMMRITA YSDRLIDDLE LLDWTEKVKS MQRNWIGRSR
310 320 330 340 350
GAEVDFSAEG ETVTVFTTRP DTLFGATYMV LAPEHELVDV LLEKAGSYEG
360 370 380 390 400
VDARWTNGQA SPAEAVAAYR ASIAAKSDLE RQENKEKTGV FLGVYATNPV
410 420 430 440 450
NGDQITVFIA DYVLTGYGTG AIMAVPAHDE RDYEFATVLG LPIKEVVAGG
460 470 480 490 500
NIEEAAFTES GEAVNSANDN GLDINGLAKD EAIAKTIEWL EEKELGRGTI
510 520 530 540 550
QYKLRDWLFA RQRYWGEPFP IVYDENGQAH ALPDSMLPVE LPEVEDYKPV
560 570 580 590 600
SFDPEDADSE PSPPLAKARE WVEVELDLGD GKKKYTRDTN VMPQWAGSSW
610 620 630 640 650
YQLRYVDPSN DEQFCNIENE RYWTGPRPET HGPNDPGGVD LYVGGVEHAV
660 670 680 690 700
LHLLYARFWH KVLFDLGHVS SKEPYRRLYN QGYIQAFAYT DSRGVYVPAD
710 720 730 740 750
DVEEKDGKFF YQGEEVNQEY GKMGKSLKNA VAPDDICNNF GADTLRVYEM
760 770 780 790 800
AMGPLDTSRP WATKDVVGAQ RFLQRLWRLV VDENTGEVLT RDEVLTDDDN
810 820 830 840 850
KQLHRTIAGV RDDYTNLRVN TVVAKLIEYV NYLTKTYPDT IPAGAVLPLI
860 870 880 890 900
VMVSPIAPHI AEELWKKLGH DDTVTYEPFP TFEEKWLTDD EIELPVQVNG
910 920 930 940 950
KVRGRITVAA DASQEQVIEA ALADEKVQEQ ISGKNLIKQI VVPGRMVNLV

VK
Length:952
Mass (Da):106,779
Last modified:October 1, 2002 - v1
Checksum:i3A1D2E2CD12D13E5
GO

Sequence cautioni

The sequence CAF18958.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAC00412.1.
BX927157 Genomic DNA. Translation: CAF18958.1. Different initiation.
RefSeqiNP_602213.1. NC_003450.3.
WP_011015573.1. NC_003450.3.
WP_011266058.1. NC_006958.1.
YP_227268.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAC00412; BAC00412; BAC00412.
CAF18958; CAF18958; cg3346.
GeneIDi1020959.
KEGGicgb:cg3346.
cgl:NCgl2915.
PATRICi21498090. VBICorGlu203724_2953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAC00412.1.
BX927157 Genomic DNA. Translation: CAF18958.1. Different initiation.
RefSeqiNP_602213.1. NC_003450.3.
WP_011015573.1. NC_003450.3.
WP_011266058.1. NC_006958.1.
YP_227268.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NLC4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.NCgl2915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC00412; BAC00412; BAC00412.
CAF18958; CAF18958; cg3346.
GeneIDi1020959.
KEGGicgb:cg3346.
cgl:NCgl2915.
PATRICi21498090. VBICorGlu203724_2953.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-2998-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiSYL_CORGL
AccessioniPrimary (citable) accession number: Q8NLC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 1, 2002
Last modified: June 24, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.