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Q8NLC4 (SYL_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Cgl3018, cg3346
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length952 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence CAF18958.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 952952Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152007

Regions

Motif66 – 7712"HIGH" region HAMAP-Rule MF_00049
Motif722 – 7265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8NLC4 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 3A1D2E2CD12D13E5

FASTA952106,779
        10         20         30         40         50         60 
MTNPSEGTTP LAFRYTPELA NKIEGEWQNY WTDNGTFNAP NPVGDLAPAD GKALPEDKLF 

        70         80         90        100        110        120 
VQDMFPYPSG AGLHVGHPLG YIATDVFARY NRMLGKNVLH TLGYDAFGLP AEQYAIQTGT 

       130        140        150        160        170        180 
HPRTTTMANI ENMKRQLGAL GLGHDSRRAV ATTDPEFYKW TQWIFLQIFN SWFDAEQQKA 

       190        200        210        220        230        240 
RPISELIPLL ESGELKTKDG ADYNALGDVE KQKAVDDYRL VYRSNSTVNW CPGLGTVLAN 

       250        260        270        280        290        300 
EEVTADGRSE RGNFPVFRKN LSQWMMRITA YSDRLIDDLE LLDWTEKVKS MQRNWIGRSR 

       310        320        330        340        350        360 
GAEVDFSAEG ETVTVFTTRP DTLFGATYMV LAPEHELVDV LLEKAGSYEG VDARWTNGQA 

       370        380        390        400        410        420 
SPAEAVAAYR ASIAAKSDLE RQENKEKTGV FLGVYATNPV NGDQITVFIA DYVLTGYGTG 

       430        440        450        460        470        480 
AIMAVPAHDE RDYEFATVLG LPIKEVVAGG NIEEAAFTES GEAVNSANDN GLDINGLAKD 

       490        500        510        520        530        540 
EAIAKTIEWL EEKELGRGTI QYKLRDWLFA RQRYWGEPFP IVYDENGQAH ALPDSMLPVE 

       550        560        570        580        590        600 
LPEVEDYKPV SFDPEDADSE PSPPLAKARE WVEVELDLGD GKKKYTRDTN VMPQWAGSSW 

       610        620        630        640        650        660 
YQLRYVDPSN DEQFCNIENE RYWTGPRPET HGPNDPGGVD LYVGGVEHAV LHLLYARFWH 

       670        680        690        700        710        720 
KVLFDLGHVS SKEPYRRLYN QGYIQAFAYT DSRGVYVPAD DVEEKDGKFF YQGEEVNQEY 

       730        740        750        760        770        780 
GKMGKSLKNA VAPDDICNNF GADTLRVYEM AMGPLDTSRP WATKDVVGAQ RFLQRLWRLV 

       790        800        810        820        830        840 
VDENTGEVLT RDEVLTDDDN KQLHRTIAGV RDDYTNLRVN TVVAKLIEYV NYLTKTYPDT 

       850        860        870        880        890        900 
IPAGAVLPLI VMVSPIAPHI AEELWKKLGH DDTVTYEPFP TFEEKWLTDD EIELPVQVNG 

       910        920        930        940        950 
KVRGRITVAA DASQEQVIEA ALADEKVQEQ ISGKNLIKQI VVPGRMVNLV VK 

« Hide

References

[1]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000036 Genomic DNA. Translation: BAC00412.1.
BX927157 Genomic DNA. Translation: CAF18958.1. Different initiation.
RefSeqNP_602213.1. NC_003450.3.
YP_227268.1. NC_006958.1.

3D structure databases

ProteinModelPortalQ8NLC4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg3346.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC00412; BAC00412; BAC00412.
CAF18958; CAF18958; cg3346.
GeneID1020959.
KEGGcgb:cg3346.
cgl:NCgl2915.
PATRIC21498090. VBICorGlu203724_2953.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAKGNPLES.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCGLU196627:GJDM-2998-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CORGL
AccessionPrimary (citable) accession number: Q8NLC4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries