Reviewed, UniProtKB/Swiss-Prot Q8NKX1 (HASA_STRP8)

Names and origin

Protein names

Recommended name:
    Hyaluronan synthase
    EC=2.4.1.212
Alternative name(s):
    Hyaluronate synthase
    Hyaluronic acid synthase
      Short name=HA synthase

Gene names

Name:	hasA
Ordered Locus Names:	spyM18_2236

Organism

Streptococcus pyogenes serotype M18 [Complete proteome] [HAMAP]

Taxonomic identifier

301451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Glycosaminoglycan synthesis. The hyaluronic acid capsule is involved in the pathogenicity of group A Streptococci; it may be the major virulence determinant.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the nodC/HAS family.
Caution	It is uncertain whether Met-1 or Met-25 is the initiator.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Virulence
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Hyaluronan synthase		PRO_0000197168
Regions
	Transmembrane	8 – 28	21	Potential
	Transmembrane	33 – 53	21	Potential
	Transmembrane	318 – 338	21	Potential
	Transmembrane	345 – 365	21	Potential
	Transmembrane	376 – 396	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8NKX1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: EB173041DA93A71B	FASTA	419	47,900
	10 20 30 40 50 60 MPIFKKTLIV LSFIFLISIL IYLNMYLFGT STVGIYGVIL ITYLVIKLGL SFLYEPFKGK 70 80 90 100 110 120 PHDYKVAAVI PSYNEDAESL LETLKSVLAQ TYPLSEIYIV DDGSSNTDAI QLIEEYVNRE 130 140 150 160 170 180 VDICRNVIVH RSLVNKGKRH AQAWAFERSD ADVFLTVDSD TYIYPNALEE LLKSFNDETV 190 200 210 220 230 240 YAATGHLNAR NRQTNLLTRL TDIRYDNAFG VERAAQSLTG NILVCSGPLS IYRREVIIPN 250 260 270 280 290 300 LERYKNQTFL GLPVSIGDDR CLTNYAIDLG RTVYQSTARC DTDVPFQLKS YLKQQNRWNK 310 320 330 340 350 360 SFFRESIISV KKILSNPIVA LWTIFEVVMF MMLIVAIGNL LFNQAIQLDL IKLFAFLSII 370 380 390 400 410 FIVALCRNVH YMVKHPASFL LSPLYGILHL FVLQPLKLYS LCTIKNTEWG TRKKVTIFK

References

[1]

"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks." Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed: 11917108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MGAS8232 / Serotype M18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009949 Genomic DNA. Translation: AAL98667.1.
PIR	A53100.
RefSeq	NP_608168.1.
3D structure databases
SMR	Q8NKX1. Positions 65-274.
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	994162.
GenomeReviews	Gene locus spyM18_2236 in contig AE009949_GR.
KEGG	spm:spyM18_2236.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG339192.
OMA	MIKHPAS.
Enzyme and pathway databases
BioCyc	SPYO186103:SPYM18_2236-MONOMER.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HASA_STRP8

Accession

Primary (citable) accession number: Q8NKX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)