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Q8NKR7 (SYL_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:TK1461
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length967 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 967967Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152141

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif650 – 6545"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6531ATP By similarity

Experimental info

Sequence conflict3211V → C in BAC10608. Ref.1
Sequence conflict5901T → A in BAC10608. Ref.1
Sequence conflict654 – 6552SK → RQ Ref.1
Sequence conflict6571N → Q Ref.1
Sequence conflict6591L → Q Ref.1
Sequence conflict6631D → Y in BAC10608. Ref.1
Sequence conflict8221E → D in BAC10608. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8NKR7 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 13A9161049DC233C

FASTA967113,135
        10         20         30         40         50         60 
MAELNFKAIE EKWQKRWMEA RVFEPDRKAK PKEKKFYITV AFPYLSGHLH VGHARTYTIP 

        70         80         90        100        110        120 
DVIARFKRMQ GYNVLFPMGW HITGAPIVGI AERIKNRDPK TIHIYRDVYK VPEEILWKFE 

       130        140        150        160        170        180 
DPKEIVKYFM KAAKETFIRA GFSVDWTREF HTTSLFPPFS KFIEWQFWTL KDMGLVVKGA 

       190        200        210        220        230        240 
HRVRWDPVVG TPLGDHDIME GEDVQILEYV IIKFILEENG ETIYLPAATL RPETVYGVTN 

       250        260        270        280        290        300 
MWLNPEATYV KAKVRKGDRE ELWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVKNPV 

       310        320        330        340        350        360 
TGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHIALEDLK KETEILLKYD IDPRVVEEIS 

       370        380        390        400        410        420 
YISLISLEGY GEFPAVEEAE RLGVKSQKDK EKLEQATKNI YKAEYHKGIF KIEPYAGKPV 

       430        440        450        460        470        480 
QEVKELVAKE MMEKGIAEIM YEFADKPVIS RFGNQAVIKI IHDQWFIDYG NPEWKAKARE 

       490        500        510        520        530        540 
ALANMTIYPE SRRAQFEAVI DWLDKKACAR KVGLGTPLPW DPDWVIESLS DSTIYMAYYT 

       550        560        570        580        590        600 
ISRHINQLRK EGKLDAEKLD REFFDYIFRE PFSEEKERKL SEKTGIPAET IHEMKEEFEY 

       610        620        630        640        650        660 
WYPLDWRCSA KDLIPNHLTF FIFNHVAIFD KKHWPKGIAV NGFGTLEGQK MSKSKGNVLN 

       670        680        690        700        710        720 
FIDAIEENGA DVVRLYIMGL AEHDSDFDWR RKEVGKLRKQ VERFYELVSE FATYEAKEGV 

       730        740        750        760        770        780 
ELKDIDRWML HRLNKAIEGA TKALEEFRTR TAVQWAFYTV LNDLRWYLRR TEGRDDDAKR 

       790        800        810        820        830        840 
YVLRTLADVW VRLMAPFTPH ISEELWEKLG GEGFVSLAPW PEPVPEWWNE TVEAEEDFVK 

       850        860        870        880        890        900 
SLIEDIKEII TVAKIENPKR AYIYTAPEWK WRVVEVVAEK RDFKAAMAEL MKDPEMRKHG 

       910        920        930        940        950        960 
KEVSKLIQRL IKERAFEVKR IDEEKALREA KDFIEKELGI EIIINPEEDR GGKKKQAMPL 


KPAVFVE 

« Hide

References

« Hide 'large scale' references
[1]"Thermococcus kodakaraensis KOD1 LeuRS gene for leucyl-tRNA synthetase."
Sawaki T., Amano H., Shiraki K., Fukuzawa K., Fujiwara S., Sekiguchi T., Takagi M.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB090309 Genomic DNA. Translation: BAC10608.1.
AP006878 Genomic DNA. Translation: BAD85650.1.
RefSeqYP_183874.1. NC_006624.1.

3D structure databases

ProteinModelPortalQ8NKR7.
SMRQ8NKR7. Positions 4-967.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8NKR7. 119 interactions.
MINTMINT-8376623.
STRING69014.TK1461.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD85650; BAD85650; TK1461.
GeneID3234295.
KEGGtko:TK1461.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAALANMTI.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycTKOD69014:GH72-1486-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THEKO
AccessionPrimary (citable) accession number: Q8NKR7
Secondary accession number(s): Q5JH65
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 29, 2005
Last modified: April 16, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries