Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8NKR7

- SYL_THEKO

UniProt

Q8NKR7 - SYL_THEKO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei653 – 6531ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1486-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:TK1461
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Leucine--tRNA ligasePRO_0000152141Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiQ8NKR7. 119 interactions.
MINTiMINT-8376623.
STRINGi69014.TK1461.

Structurei

3D structure databases

ProteinModelPortaliQ8NKR7.
SMRiQ8NKR7. Positions 4-967.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi650 – 6545"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
InParanoidiQ8NKR7.
KOiK01869.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NKR7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAELNFKAIE EKWQKRWMEA RVFEPDRKAK PKEKKFYITV AFPYLSGHLH
60 70 80 90 100
VGHARTYTIP DVIARFKRMQ GYNVLFPMGW HITGAPIVGI AERIKNRDPK
110 120 130 140 150
TIHIYRDVYK VPEEILWKFE DPKEIVKYFM KAAKETFIRA GFSVDWTREF
160 170 180 190 200
HTTSLFPPFS KFIEWQFWTL KDMGLVVKGA HRVRWDPVVG TPLGDHDIME
210 220 230 240 250
GEDVQILEYV IIKFILEENG ETIYLPAATL RPETVYGVTN MWLNPEATYV
260 270 280 290 300
KAKVRKGDRE ELWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVKNPV
310 320 330 340 350
TGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHIALEDLK KETEILLKYD
360 370 380 390 400
IDPRVVEEIS YISLISLEGY GEFPAVEEAE RLGVKSQKDK EKLEQATKNI
410 420 430 440 450
YKAEYHKGIF KIEPYAGKPV QEVKELVAKE MMEKGIAEIM YEFADKPVIS
460 470 480 490 500
RFGNQAVIKI IHDQWFIDYG NPEWKAKARE ALANMTIYPE SRRAQFEAVI
510 520 530 540 550
DWLDKKACAR KVGLGTPLPW DPDWVIESLS DSTIYMAYYT ISRHINQLRK
560 570 580 590 600
EGKLDAEKLD REFFDYIFRE PFSEEKERKL SEKTGIPAET IHEMKEEFEY
610 620 630 640 650
WYPLDWRCSA KDLIPNHLTF FIFNHVAIFD KKHWPKGIAV NGFGTLEGQK
660 670 680 690 700
MSKSKGNVLN FIDAIEENGA DVVRLYIMGL AEHDSDFDWR RKEVGKLRKQ
710 720 730 740 750
VERFYELVSE FATYEAKEGV ELKDIDRWML HRLNKAIEGA TKALEEFRTR
760 770 780 790 800
TAVQWAFYTV LNDLRWYLRR TEGRDDDAKR YVLRTLADVW VRLMAPFTPH
810 820 830 840 850
ISEELWEKLG GEGFVSLAPW PEPVPEWWNE TVEAEEDFVK SLIEDIKEII
860 870 880 890 900
TVAKIENPKR AYIYTAPEWK WRVVEVVAEK RDFKAAMAEL MKDPEMRKHG
910 920 930 940 950
KEVSKLIQRL IKERAFEVKR IDEEKALREA KDFIEKELGI EIIINPEEDR
960
GGKKKQAMPL KPAVFVE
Length:967
Mass (Da):113,135
Last modified:March 29, 2005 - v2
Checksum:i13A9161049DC233C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti321 – 3211V → C in BAC10608. 1 PublicationCurated
Sequence conflicti590 – 5901T → A in BAC10608. 1 PublicationCurated
Sequence conflicti654 – 6552SK → RQ1 PublicationCurated
Sequence conflicti657 – 6571N → Q1 PublicationCurated
Sequence conflicti659 – 6591L → Q1 PublicationCurated
Sequence conflicti663 – 6631D → Y in BAC10608. 1 PublicationCurated
Sequence conflicti822 – 8221E → D in BAC10608. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB090309 Genomic DNA. Translation: BAC10608.1.
AP006878 Genomic DNA. Translation: BAD85650.1.
RefSeqiYP_183874.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85650; BAD85650; TK1461.
GeneIDi3234295.
KEGGitko:TK1461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB090309 Genomic DNA. Translation: BAC10608.1 .
AP006878 Genomic DNA. Translation: BAD85650.1 .
RefSeqi YP_183874.1. NC_006624.1.

3D structure databases

ProteinModelPortali Q8NKR7.
SMRi Q8NKR7. Positions 4-967.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8NKR7. 119 interactions.
MINTi MINT-8376623.
STRINGi 69014.TK1461.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD85650 ; BAD85650 ; TK1461 .
GeneIDi 3234295.
KEGGi tko:TK1461.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216620.
InParanoidi Q8NKR7.
KOi K01869.

Enzyme and pathway databases

BioCyci TKOD69014:GH72-1486-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_A. Leu_tRNA_synth_A.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Thermococcus kodakaraensis KOD1 LeuRS gene for leucyl-tRNA synthetase."
    Sawaki T., Amano H., Shiraki K., Fukuzawa K., Fujiwara S., Sekiguchi T., Takagi M.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.
  2. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiSYL_THEKO
AccessioniPrimary (citable) accession number: Q8NKR7
Secondary accession number(s): Q5JH65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 29, 2005
Last modified: October 29, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3