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Q8NKR7

- SYL_THEKO

UniProt

Q8NKR7 - SYL_THEKO

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei653 – 6531ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTKOD69014:GH72-1486-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:TK1461
    OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
    Taxonomic identifieri69014 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000000536: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 967967Leucine--tRNA ligasePRO_0000152141Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    IntActiQ8NKR7. 119 interactions.
    MINTiMINT-8376623.
    STRINGi69014.TK1461.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NKR7.
    SMRiQ8NKR7. Positions 4-967.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi43 – 5311"HIGH" regionAdd
    BLAST
    Motifi650 – 6545"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000216620.
    KOiK01869.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_A. Leu_tRNA_synth_A.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR020791. Leu-tRNA-lgase_arc.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8NKR7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAELNFKAIE EKWQKRWMEA RVFEPDRKAK PKEKKFYITV AFPYLSGHLH    50
    VGHARTYTIP DVIARFKRMQ GYNVLFPMGW HITGAPIVGI AERIKNRDPK 100
    TIHIYRDVYK VPEEILWKFE DPKEIVKYFM KAAKETFIRA GFSVDWTREF 150
    HTTSLFPPFS KFIEWQFWTL KDMGLVVKGA HRVRWDPVVG TPLGDHDIME 200
    GEDVQILEYV IIKFILEENG ETIYLPAATL RPETVYGVTN MWLNPEATYV 250
    KAKVRKGDRE ELWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVKNPV 300
    TGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHIALEDLK KETEILLKYD 350
    IDPRVVEEIS YISLISLEGY GEFPAVEEAE RLGVKSQKDK EKLEQATKNI 400
    YKAEYHKGIF KIEPYAGKPV QEVKELVAKE MMEKGIAEIM YEFADKPVIS 450
    RFGNQAVIKI IHDQWFIDYG NPEWKAKARE ALANMTIYPE SRRAQFEAVI 500
    DWLDKKACAR KVGLGTPLPW DPDWVIESLS DSTIYMAYYT ISRHINQLRK 550
    EGKLDAEKLD REFFDYIFRE PFSEEKERKL SEKTGIPAET IHEMKEEFEY 600
    WYPLDWRCSA KDLIPNHLTF FIFNHVAIFD KKHWPKGIAV NGFGTLEGQK 650
    MSKSKGNVLN FIDAIEENGA DVVRLYIMGL AEHDSDFDWR RKEVGKLRKQ 700
    VERFYELVSE FATYEAKEGV ELKDIDRWML HRLNKAIEGA TKALEEFRTR 750
    TAVQWAFYTV LNDLRWYLRR TEGRDDDAKR YVLRTLADVW VRLMAPFTPH 800
    ISEELWEKLG GEGFVSLAPW PEPVPEWWNE TVEAEEDFVK SLIEDIKEII 850
    TVAKIENPKR AYIYTAPEWK WRVVEVVAEK RDFKAAMAEL MKDPEMRKHG 900
    KEVSKLIQRL IKERAFEVKR IDEEKALREA KDFIEKELGI EIIINPEEDR 950
    GGKKKQAMPL KPAVFVE 967
    Length:967
    Mass (Da):113,135
    Last modified:March 29, 2005 - v2
    Checksum:i13A9161049DC233C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti321 – 3211V → C in BAC10608. 1 PublicationCurated
    Sequence conflicti590 – 5901T → A in BAC10608. 1 PublicationCurated
    Sequence conflicti654 – 6552SK → RQ1 PublicationCurated
    Sequence conflicti657 – 6571N → Q1 PublicationCurated
    Sequence conflicti659 – 6591L → Q1 PublicationCurated
    Sequence conflicti663 – 6631D → Y in BAC10608. 1 PublicationCurated
    Sequence conflicti822 – 8221E → D in BAC10608. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB090309 Genomic DNA. Translation: BAC10608.1.
    AP006878 Genomic DNA. Translation: BAD85650.1.
    RefSeqiYP_183874.1. NC_006624.1.

    Genome annotation databases

    EnsemblBacteriaiBAD85650; BAD85650; TK1461.
    GeneIDi3234295.
    KEGGitko:TK1461.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB090309 Genomic DNA. Translation: BAC10608.1 .
    AP006878 Genomic DNA. Translation: BAD85650.1 .
    RefSeqi YP_183874.1. NC_006624.1.

    3D structure databases

    ProteinModelPortali Q8NKR7.
    SMRi Q8NKR7. Positions 4-967.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8NKR7. 119 interactions.
    MINTi MINT-8376623.
    STRINGi 69014.TK1461.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD85650 ; BAD85650 ; TK1461 .
    GeneIDi 3234295.
    KEGGi tko:TK1461.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000216620.
    KOi K01869.

    Enzyme and pathway databases

    BioCyci TKOD69014:GH72-1486-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_A. Leu_tRNA_synth_A.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR020791. Leu-tRNA-lgase_arc.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Thermococcus kodakaraensis KOD1 LeuRS gene for leucyl-tRNA synthetase."
      Sawaki T., Amano H., Shiraki K., Fukuzawa K., Fujiwara S., Sekiguchi T., Takagi M.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.
    2. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
      Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
      Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.

    Entry informationi

    Entry nameiSYL_THEKO
    AccessioniPrimary (citable) accession number: Q8NKR7
    Secondary accession number(s): Q5JH65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2003
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3