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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (tpiA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93ElectrophileUniRule annotation1
Active sitei141Proton acceptorUniRule annotation1
Binding sitei146Substrate; via carbonyl oxygenUniRule annotation1
Binding sitei181Substrate; via amide nitrogenUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciTTEN768679:G1H3R-506-MONOMER
BRENDAi5.3.1.1 6329
UniPathwayiUPA00109; UER00189
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomeraseUniRule annotation (EC:5.3.1.1UniRule annotation)
Short name:
TIMUniRule annotation
Short name:
TPIUniRule annotation
Alternative name(s):
Triose-phosphate isomeraseUniRule annotation
Gene namesi
Name:tpiAUniRule annotation
Synonyms:tpi
Ordered Locus Names:TTX_0494
OrganismiThermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1)
Taxonomic identifieri768679 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaeThermoproteus
Proteomesi
  • UP000002654 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationCurated

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000903491 – 226Triosephosphate isomeraseAdd BLAST226

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi768679.TTX_0494

Structurei

Secondary structure

1226
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi14 – 16Combined sources3
Helixi19 – 35Combined sources17
Beta strandi38 – 42Combined sources5
Helixi45 – 47Combined sources3
Helixi48 – 52Combined sources5
Beta strandi59 – 62Combined sources4
Beta strandi65 – 68Combined sources4
Helixi78 – 84Combined sources7
Beta strandi88 – 91Combined sources4
Helixi100 – 112Combined sources13
Beta strandi116 – 123Combined sources8
Helixi124 – 132Combined sources9
Beta strandi136 – 140Combined sources5
Helixi143 – 145Combined sources3
Turni146 – 148Combined sources3
Helixi152 – 155Combined sources4
Helixi157 – 170Combined sources14
Beta strandi174 – 181Combined sources8
Helixi185 – 193Combined sources9
Beta strandi197 – 201Combined sources5
Helixi203 – 206Combined sources4
Helixi211 – 224Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W0MX-ray2.50A/B/C/D/E/F/G/H1-226[»]
ProteinModelPortaliQ8NKN9
SMRiQ8NKN9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NKN9

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 11Substrate bindingUniRule annotation3
Regioni202 – 203Substrate bindingUniRule annotation1 Publication2

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01087 Archaea
COG0149 LUCA
KOiK01803
OMAiIPVYAQH

Family and domain databases

CDDicd00311 TIM, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00147_A TIM_A, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR035990 TIM_sf
IPR000652 Triosephosphate_isomerase
IPR022891 Triosephosphate_isomerase_arc
IPR020861 Triosephosphate_isomerase_AS
PANTHERiPTHR21139 PTHR21139, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit
PF00121 TIM, 1 hit
SUPFAMiSSF51351 SSF51351, 1 hit
TIGRFAMsiTIGR00419 tim, 1 hit
PROSITEiView protein in PROSITE
PS00171 TIM_1, 1 hit
PS51440 TIM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8NKN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLPILIINF KAYGEAAGKR AVELAKAAER AARELGVNIV VAPNHLELGL
60 70 80 90 100
VSQSVDIPVY AQGADVEAGG AHTAHVSLEN IKEAGGSGVI LNHSEAPLKL
110 120 130 140 150
NDLARLVAKA KSLGLDVVVC APDPRTSLAA AALGPHAVAV EPPELIGTGR
160 170 180 190 200
AVSRYKPEAI VETVGLVSRH FPEVSVITGA GIESGDDVAA ALRLGTRGVL
210 220
LASAAVKAKD PYAKIVELAK PLSELR
Length:226
Mass (Da):23,283
Last modified:October 1, 2002 - v1
Checksum:i53BE6A0A5F492857
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012066 Genomic DNA Translation: CAB66162.1
AJ515539 Genomic DNA Translation: CAD56500.1
FN869859 Genomic DNA Translation: CCC81161.1
RefSeqiWP_014126418.1, NC_016070.1

Genome annotation databases

EnsemblBacteriaiCCC81161; CCC81161; TTX_0494
GeneIDi11263496
KEGGittn:TTX_0494
PATRICifig|768679.9.peg.509

Similar proteinsi

Entry informationi

Entry nameiTPIS_THETK
AccessioniPrimary (citable) accession number: Q8NKN9
Secondary accession number(s): G4RNL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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