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Q8NKE1 (GLGB_RHIID) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan-branching enzyme

EC=2.4.1.18
Alternative name(s):
Glycogen-branching enzyme
Gene names
Name:GLC3
ORF Names:GLOINDRAFT_70859
OrganismRhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) (Arbuscular mycorrhizal fungus) (Glomus intraradices) [Complete proteome]
Taxonomic identifier747089 [NCBI]
Taxonomic lineageEukaryotaFungiGlomeromycotaGlomeromycetesGlomeralesGlomeraceaeRhizophagus

Protein attributes

Sequence length683 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway

Glycan biosynthesis; glycogen biosynthesis.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence caution

The sequence AAM33305.1 differs from that shown. Reason: Frameshift at positions 474 and 483.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6836831,4-alpha-glucan-branching enzyme
PRO_0000188783

Sites

Active site3421Nucleophile By similarity
Active site3971Proton donor By similarity

Experimental info

Sequence conflict474 – 48310TLAFWLMDKE → NTCLLVNGQG in AAM33305. Ref.1
Sequence conflict6131H → L in AAM33305. Ref.1
Sequence conflict6781L → P in AAM33305. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8NKE1 [UniParc].

Last modified March 19, 2014. Version 2.
Checksum: 0AABB4601D69D1CF

FASTA68379,142
        10         20         30         40         50         60 
MSPTEMISQE NYEPGICKID PWLKPFAPAI KRRLESYKKW VKEINQNEGG YDKFSRGYER 

        70         80         90        100        110        120 
FGLNVLPNGD IIYREWAPNA VAASLIGEFN DWDRSKHPMK KDSFGVWEVH IPAKNGIPTI 

       130        140        150        160        170        180 
PHNTKIKISM TTPEGECIDR LPAWIKRVTQ DLNVSLAYDA IFWNPPQKYQ WKNNSPKKPT 

       190        200        210        220        230        240 
SLRIYEAHVG ISTNEGRVGT YNEFTDNVLK RIKDLGYNAI QLMAIMEHAY YASFGYQVTS 

       250        260        270        280        290        300 
FFGVSSRYGT PEELMRLIDT AHGMGLYVLL DVVHSHACKN VLDGLNMFDG SDHCYFHEGG 

       310        320        330        340        350        360 
KGRHDLWDSR LFNYGHWEVL RFLLSNLRFF MEEYRFDGFR FDGVTSMMYH HHGIGTGFSG 

       370        380        390        400        410        420 
GYHEYFGDTV DEGGVVYLML ANDMLHKLYP RIITVSEDVS GMPGLCLPVE EGGIGFDYRL 

       430        440        450        460        470        480 
AMAIPDMWIK LLKEQRDDDW DMGNICWTLT NRRHMEKTIA YAESHDQALV GDKTLAFWLM 

       490        500        510        520        530        540 
DKEMYTHMSD MTPLTPIIDR GLALHKMIRL LTHGLGGEGY LNFEGNEFGH PEWLDFPRAG 

       550        560        570        580        590        600 
NNNSFHYARR QWNVVDDDLL RYKYLNEFDK AMQHLEEQYG WLSSPQAYIS LKHNENKLVA 

       610        620        630        640        650        660 
FERGNLLWIF NFHPTQSFAD YKIGTEWAGK YSIALNTDRK IFGGHDRIDE SISYHSQPHE 

       670        680 
WDGRKNYIQV YIPCRVALVL SHC 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF503447 mRNA. Translation: AAM33305.1. Frameshift.
KI299226 Genomic DNA. Translation: ERZ97888.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_RHIID
AccessionPrimary (citable) accession number: Q8NKE1
Secondary accession number(s): U9SPI3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 19, 2014
Last modified: July 9, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries