Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,4-alpha-glucan-branching enzyme

Gene

GLC3

Organism
Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) (Arbuscular mycorrhizal fungus) (Glomus intraradices)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway:iglycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei342 – 3421NucleophileBy similarity
Active sitei397 – 3971Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Glycogen-branching enzyme
Gene namesi
Name:GLC3
ORF Names:GLOINDRAFT_70859
OrganismiRhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) (Arbuscular mycorrhizal fungus) (Glomus intraradices)
Taxonomic identifieri747089 [NCBI]
Taxonomic lineageiEukaryotaFungiGlomeromycotaGlomeromycetesGlomeralesGlomeraceaeRhizophagus
ProteomesiUP000018888 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6836831,4-alpha-glucan-branching enzymePRO_0000188783Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NKE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPTEMISQE NYEPGICKID PWLKPFAPAI KRRLESYKKW VKEINQNEGG
60 70 80 90 100
YDKFSRGYER FGLNVLPNGD IIYREWAPNA VAASLIGEFN DWDRSKHPMK
110 120 130 140 150
KDSFGVWEVH IPAKNGIPTI PHNTKIKISM TTPEGECIDR LPAWIKRVTQ
160 170 180 190 200
DLNVSLAYDA IFWNPPQKYQ WKNNSPKKPT SLRIYEAHVG ISTNEGRVGT
210 220 230 240 250
YNEFTDNVLK RIKDLGYNAI QLMAIMEHAY YASFGYQVTS FFGVSSRYGT
260 270 280 290 300
PEELMRLIDT AHGMGLYVLL DVVHSHACKN VLDGLNMFDG SDHCYFHEGG
310 320 330 340 350
KGRHDLWDSR LFNYGHWEVL RFLLSNLRFF MEEYRFDGFR FDGVTSMMYH
360 370 380 390 400
HHGIGTGFSG GYHEYFGDTV DEGGVVYLML ANDMLHKLYP RIITVSEDVS
410 420 430 440 450
GMPGLCLPVE EGGIGFDYRL AMAIPDMWIK LLKEQRDDDW DMGNICWTLT
460 470 480 490 500
NRRHMEKTIA YAESHDQALV GDKTLAFWLM DKEMYTHMSD MTPLTPIIDR
510 520 530 540 550
GLALHKMIRL LTHGLGGEGY LNFEGNEFGH PEWLDFPRAG NNNSFHYARR
560 570 580 590 600
QWNVVDDDLL RYKYLNEFDK AMQHLEEQYG WLSSPQAYIS LKHNENKLVA
610 620 630 640 650
FERGNLLWIF NFHPTQSFAD YKIGTEWAGK YSIALNTDRK IFGGHDRIDE
660 670 680
SISYHSQPHE WDGRKNYIQV YIPCRVALVL SHC
Length:683
Mass (Da):79,142
Last modified:March 19, 2014 - v2
Checksum:i0AABB4601D69D1CF
GO

Sequence cautioni

The sequence AAM33305.1 differs from that shown. Reason: Frameshift at positions 474 and 483. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti474 – 48310TLAFWLMDKE → NTCLLVNGQG in AAM33305 (PubMed:12644699).Curated
Sequence conflicti613 – 6131H → L in AAM33305 (PubMed:12644699).Curated
Sequence conflicti678 – 6781L → P in AAM33305 (PubMed:12644699).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503447 mRNA. Translation: AAM33305.1. Frameshift.
KI299226 Genomic DNA. Translation: ERZ97888.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503447 mRNA. Translation: AAM33305.1. Frameshift.
KI299226 Genomic DNA. Translation: ERZ97888.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Carbon export from arbuscular mycorrhizal roots involves the translocation of carbohydrate as well as lipid."
    Bago B., Pfeffer P.E., Abubaker J., Jun J., Allen J.W., Brouillette J., Douds D.D., Lammers P.J., Shachar-Hill Y.
    Plant Physiol. 131:1496-1507(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: DAOM 181602 / DAOM 197198 / MUCL 43194.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 24-683.
    Strain: DAOM 181602 / DAOM 197198 / MUCL 43194.

Entry informationi

Entry nameiGLGB_RHIID
AccessioniPrimary (citable) accession number: Q8NKE1
Secondary accession number(s): U9SPI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 19, 2014
Last modified: January 7, 2015
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.