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Protein

1,4-alpha-glucan-branching enzyme

Gene

GLC3

Organism
Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) (Arbuscular mycorrhizal fungus) (Glomus intraradices)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei342NucleophileBy similarity1
Active sitei397Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processGlycogen biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Glycogen-branching enzyme
Gene namesi
Name:GLC3
ORF Names:GLOINDRAFT_70859
OrganismiRhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) (Arbuscular mycorrhizal fungus) (Glomus intraradices)
Taxonomic identifieri747089 [NCBI]
Taxonomic lineageiEukaryotaFungiMucoromycotaGlomeromycotinaGlomeromycetesGlomeralesGlomeraceaeRhizophagus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887831 – 6831,4-alpha-glucan-branching enzymeAdd BLAST683

Structurei

3D structure databases

SMRiQ8NKE1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Q8NKE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPTEMISQE NYEPGICKID PWLKPFAPAI KRRLESYKKW VKEINQNEGG
60 70 80 90 100
YDKFSRGYER FGLNVLPNGD IIYREWAPNA VAASLIGEFN DWDRSKHPMK
110 120 130 140 150
KDSFGVWEVH IPAKNGIPTI PHNTKIKISM TTPEGECIDR LPAWIKRVTQ
160 170 180 190 200
DLNVSLAYDA IFWNPPQKYQ WKNNSPKKPT SLRIYEAHVG ISTNEGRVGT
210 220 230 240 250
YNEFTDNVLK RIKDLGYNAI QLMAIMEHAY YASFGYQVTS FFGVSSRYGT
260 270 280 290 300
PEELMRLIDT AHGMGLYVLL DVVHSHACKN VLDGLNMFDG SDHCYFHEGG
310 320 330 340 350
KGRHDLWDSR LFNYGHWEVL RFLLSNLRFF MEEYRFDGFR FDGVTSMMYH
360 370 380 390 400
HHGIGTGFSG GYHEYFGDTV DEGGVVYLML ANDMLHKLYP RIITVSEDVS
410 420 430 440 450
GMPGLCLPVE EGGIGFDYRL AMAIPDMWIK LLKEQRDDDW DMGNICWTLT
460 470 480 490 500
NRRHMEKTIA YAESHDQALV GDKTLAFWLM DKEMYTHMSD MTPLTPIIDR
510 520 530 540 550
GLALHKMIRL LTHGLGGEGY LNFEGNEFGH PEWLDFPRAG NNNSFHYARR
560 570 580 590 600
QWNVVDDDLL RYKYLNEFDK AMQHLEEQYG WLSSPQAYIS LKHNENKLVA
610 620 630 640 650
FERGNLLWIF NFHPTQSFAD YKIGTEWAGK YSIALNTDRK IFGGHDRIDE
660 670 680
SISYHSQPHE WDGRKNYIQV YIPCRVALVL SHC
Length:683
Mass (Da):79,142
Last modified:March 19, 2014 - v2
Checksum:i0AABB4601D69D1CF
GO

Sequence cautioni

The sequence AAM33305 differs from that shown. Reason: Frameshift at positions 474 and 483.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti474 – 483TLAFWLMDKE → NTCLLVNGQG in AAM33305 (PubMed:12644699).Curated10
Sequence conflicti613H → L in AAM33305 (PubMed:12644699).Curated1
Sequence conflicti678L → P in AAM33305 (PubMed:12644699).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503447 mRNA Translation: AAM33305.1 Frameshift.
KI299226 Genomic DNA Translation: ERZ97888.1

Genome annotation databases

EnsemblFungiiERZ97888; ERZ97888; EFG00047785815

Similar proteinsi

Entry informationi

Entry nameiGLGB_RHIID
AccessioniPrimary (citable) accession number: Q8NKE1
Secondary accession number(s): U9SPI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 19, 2014
Last modified: April 25, 2018
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health