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Protein

1,4-alpha-glucan-branching enzyme

Gene

GLC3

Organism
Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) (Arbuscular mycorrhizal fungus) (Glomus intraradices)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei342NucleophileBy similarity1
Active sitei397Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processGlycogen biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Glycogen-branching enzyme
Gene namesi
Name:GLC3
ORF Names:GLOINDRAFT_70859
OrganismiRhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) (Arbuscular mycorrhizal fungus) (Glomus intraradices)
Taxonomic identifieri747089 [NCBI]
Taxonomic lineageiEukaryotaFungiMucoromycotaGlomeromycotinaGlomeromycetesGlomeralesGlomeraceaeRhizophagus
Proteomesi
  • UP000018888 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887831 – 6831,4-alpha-glucan-branching enzymeAdd BLAST683

Structurei

3D structure databases

SMRiQ8NKE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8NKE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPTEMISQE NYEPGICKID PWLKPFAPAI KRRLESYKKW VKEINQNEGG
60 70 80 90 100
YDKFSRGYER FGLNVLPNGD IIYREWAPNA VAASLIGEFN DWDRSKHPMK
110 120 130 140 150
KDSFGVWEVH IPAKNGIPTI PHNTKIKISM TTPEGECIDR LPAWIKRVTQ
160 170 180 190 200
DLNVSLAYDA IFWNPPQKYQ WKNNSPKKPT SLRIYEAHVG ISTNEGRVGT
210 220 230 240 250
YNEFTDNVLK RIKDLGYNAI QLMAIMEHAY YASFGYQVTS FFGVSSRYGT
260 270 280 290 300
PEELMRLIDT AHGMGLYVLL DVVHSHACKN VLDGLNMFDG SDHCYFHEGG
310 320 330 340 350
KGRHDLWDSR LFNYGHWEVL RFLLSNLRFF MEEYRFDGFR FDGVTSMMYH
360 370 380 390 400
HHGIGTGFSG GYHEYFGDTV DEGGVVYLML ANDMLHKLYP RIITVSEDVS
410 420 430 440 450
GMPGLCLPVE EGGIGFDYRL AMAIPDMWIK LLKEQRDDDW DMGNICWTLT
460 470 480 490 500
NRRHMEKTIA YAESHDQALV GDKTLAFWLM DKEMYTHMSD MTPLTPIIDR
510 520 530 540 550
GLALHKMIRL LTHGLGGEGY LNFEGNEFGH PEWLDFPRAG NNNSFHYARR
560 570 580 590 600
QWNVVDDDLL RYKYLNEFDK AMQHLEEQYG WLSSPQAYIS LKHNENKLVA
610 620 630 640 650
FERGNLLWIF NFHPTQSFAD YKIGTEWAGK YSIALNTDRK IFGGHDRIDE
660 670 680
SISYHSQPHE WDGRKNYIQV YIPCRVALVL SHC
Length:683
Mass (Da):79,142
Last modified:March 19, 2014 - v2
Checksum:i0AABB4601D69D1CF
GO

Sequence cautioni

The sequence AAM33305 differs from that shown. Reason: Frameshift at positions 474 and 483.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti474 – 483TLAFWLMDKE → NTCLLVNGQG in AAM33305 (PubMed:12644699).Curated10
Sequence conflicti613H → L in AAM33305 (PubMed:12644699).Curated1
Sequence conflicti678L → P in AAM33305 (PubMed:12644699).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503447 mRNA. Translation: AAM33305.1. Frameshift.
KI299226 Genomic DNA. Translation: ERZ97888.1.

Genome annotation databases

EnsemblFungiiERZ97888; ERZ97888; GLOINDRAFT_70859.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLGB_RHIID
AccessioniPrimary (citable) accession number: Q8NKE1
Secondary accession number(s): U9SPI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 19, 2014
Last modified: May 10, 2017
This is version 74 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families