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Protein

Enolase 1-2

Gene

eno102

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.By similarity

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd3), Glyceraldehyde-3-phosphate dehydrogenase 1 (tdh1)
  2. Phosphoglycerate kinase (pgk1)
  3. Phosphoglycerate mutase (gpm1)
  4. Enolase 1-2 (eno102), Enolase 1-1 (eno101)
  5. Pyruvate kinase (pyk1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei160 – 1601SubstrateBy similarity
Binding sitei169 – 1691SubstrateBy similarity
Active sitei212 – 2121Proton donorBy similarity
Metal bindingi247 – 2471MagnesiumBy similarity
Metal bindingi296 – 2961MagnesiumBy similarity
Binding sitei296 – 2961SubstrateBy similarity
Metal bindingi321 – 3211MagnesiumBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Active sitei346 – 3461Proton acceptorBy similarity
Binding sitei397 – 3971SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-70171. Glycolysis.
R-SPO-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 1-2 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 1-2
2-phosphoglycerate dehydratase 1-2
Gene namesi
Name:eno102
Synonyms:eno1
ORF Names:SPBPB21E7.01c, SPBPB8B6.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBPB21E7.01c.
PomBaseiSPBPB21E7.01c. eno102.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Enolase 1-2PRO_0000134059Add
BLAST

Proteomic databases

MaxQBiQ8NKC2.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8NKC2.
SMRiQ8NKC2. Positions 4-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni373 – 3764Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiQ8NKC2.
KOiK01689.
OMAiCESGHAC.
OrthoDBiEOG092C2W5X.
PhylomeDBiQ8NKC2.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NKC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTIQKIYSR SIYDSRGNPT VEVELTTELG TFRSMVPSGA STGEWEAKEL
60 70 80 90 100
RDNDKNKWGG KGVTIAVHNV NNIIGPALVK SDIKITDQRG IDEFMIKLDG
110 120 130 140 150
TNDKSKLGAN SIVGVSMAVA RAAAAFLKIP LYEYIGKLAG SKTTECIPVP
160 170 180 190 200
SFNVLNGGRH AGGDLAFQEF MIMPIKAPTF SEGLRWGSEV YHTLKALAKK
210 220 230 240 250
KYGASAGNVG DEGGIAPDLT TAEEALDLVN EAIKEAGYDG KVKIGFDVAA
260 270 280 290 300
SELYNGKLYD LDFKSEHPKP ENKLDYKKLY EKYSALIEKY PIVFIEDPFS
310 320 330 340 350
EEDWGAFSYM SSKTKVEVIA DDLTVTNVKR LSKAIELKCA NALLVKINQI
360 370 380 390 400
GSLSETIDAA NMAKKAGWGL MVSHRSGETD DSFIAHLAVG LEAGQMKSGA
410 420 430 440
PCRSERLAKY NELLRIEDNL GDSAIYAGTR AADYIKSNTL
Length:440
Mass (Da):47,867
Last modified:April 11, 2003 - v2
Checksum:iE59B1487C8C6B382
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAD27913.2.
RefSeqiNP_001018769.2. NM_001020944.3.

Genome annotation databases

EnsemblFungiiSPBPB21E7.01c.1; SPBPB21E7.01c.1:pep; SPBPB21E7.01c.
GeneIDi3361181.
KEGGispo:SPBPB21E7.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAD27913.2.
RefSeqiNP_001018769.2. NM_001020944.3.

3D structure databases

ProteinModelPortaliQ8NKC2.
SMRiQ8NKC2. Positions 4-428.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiQ8NKC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBPB21E7.01c.1; SPBPB21E7.01c.1:pep; SPBPB21E7.01c.
GeneIDi3361181.
KEGGispo:SPBPB21E7.01c.

Organism-specific databases

EuPathDBiFungiDB:SPBPB21E7.01c.
PomBaseiSPBPB21E7.01c. eno102.

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiQ8NKC2.
KOiK01689.
OMAiCESGHAC.
OrthoDBiEOG092C2W5X.
PhylomeDBiQ8NKC2.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
ReactomeiR-SPO-70171. Glycolysis.
R-SPO-70263. Gluconeogenesis.

Miscellaneous databases

PROiQ8NKC2.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENO12_SCHPO
AccessioniPrimary (citable) accession number: Q8NKC2
Secondary accession number(s): Q8TFF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: April 11, 2003
Last modified: September 7, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.