ID   COMT2_SCHPO             Reviewed;         281 AA.
AC   Q8NKC1;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 31.
DE   RecName: Full=Probable catechol O-methyltransferase 2;
DE            EC=2.1.1.6;
GN   ORFNames=SPBPB21E7.04c;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the
RT   fission yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + a catechol = S-
CC       adenosyl-L-homocysteine + a guaiacol.
CC   -!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Vacuole.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       Catechol-O-methyltransferase family.
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DR   EMBL; CU329671; CAD31744.1; -; Genomic_DNA.
DR   RefSeq; NP_001018770.1; -.
DR   HSSP; P22734; 1H1D.
DR   GeneID; 3361178; -.
DR   KEGG; spo:SPBPB21E7.04c; -.
DR   GeneDB_Spombe; SPBPB21E7.04c; -.
DR   OMA; Q8NKC1; ASEDWIP.
DR   BRENDA; 2.1.1.6; 653.
DR   GO; GO:0005773; C:vacuole; IDA:GeneDB_SPombe.
DR   GO; GO:0016206; F:catechol O-methyltransferase activity; IEA:EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR002935; O-MeTrfase_3.
DR   PANTHER; PTHR10509; Methyltransf_3; 1.
DR   Pfam; PF01596; Methyltransf_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Magnesium; Metal-binding; Methyltransferase;
KW   Transferase; Vacuole.
FT   CHAIN         1    281       Probable catechol O-methyltransferase 2.
FT                                /FTId=PRO_0000318149.
FT   METAL       183    183       Magnesium (By similarity).
FT   METAL       211    211       Magnesium (By similarity).
FT   METAL       212    212       Magnesium (By similarity).
FT   BINDING     108    108       S-adenosyl-L-methionine (By similarity).
FT   BINDING     127    127       S-adenosyl-L-methionine (By similarity).
FT   BINDING     183    183       S-adenosyl-L-methionine (By similarity).
FT   BINDING     186    186       Substrate (By similarity).
FT   BINDING     212    212       Substrate (By similarity).
SQ   SEQUENCE   281 AA;  32123 MW;  24022103FE9993B1 CRC64;
     MFAQALHWLT TSKLRYALAL PFLFFILYTK TKKSKEQELE NYIFSLPREK LDQIRGKPDE
     VINVIDEYVE QGHFLMNIGK LKGKIISEKI QQVKPKVMIE LGGYVGYSAI LFGKQLTDPS
     AHYYSLEVNP KFAKIASKII DLAGLSNKVT IIVGKATDSL VELRRSLPNV IPSFEYLDFV
     FIDHWKDLYV PDLRVMETLD LIGQGSIIAA DNILRPGVPE YVKYVQGSLD YRKEYDSTVS
     NVNGPQFIGK WNIIYKSKTI KVDDGKREKD AVEITEYIEA K
//