Reviewed, UniProtKB/Swiss-Prot Q8NKC1 (COMT2_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable catechol O-methyltransferase 2
    EC=2.1.1.6

Gene names

ORF Names:

SPBPB21E7.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Vacuole. Ref.2
Sequence similarities	Belongs to the methyltransferase superfamily. Catechol-O-methyltransferase family.

Ontologies

Keywords
Cellular component	Vacuole
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	vacuole Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	catechol O-methyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Probable catechol O-methyltransferase 2		PRO_0000318149
Sites
	Metal binding	183	1	Magnesium By similarity
	Metal binding	211	1	Magnesium By similarity
	Metal binding	212	1	Magnesium By similarity
	Binding site	108	1	S-adenosyl-L-methionine By similarity
	Binding site	127	1	S-adenosyl-L-methionine By similarity
	Binding site	183	1	S-adenosyl-L-methionine By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NKC1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 24022103FE9993B1	FASTA	281	32,123
	10 20 30 40 50 60 MFAQALHWLT TSKLRYALAL PFLFFILYTK TKKSKEQELE NYIFSLPREK LDQIRGKPDE 70 80 90 100 110 120 VINVIDEYVE QGHFLMNIGK LKGKIISEKI QQVKPKVMIE LGGYVGYSAI LFGKQLTDPS 130 140 150 160 170 180 AHYYSLEVNP KFAKIASKII DLAGLSNKVT IIVGKATDSL VELRRSLPNV IPSFEYLDFV 190 200 210 220 230 240 FIDHWKDLYV PDLRVMETLD LIGQGSIIAA DNILRPGVPE YVKYVQGSLD YRKEYDSTVS 250 260 270 280 NVNGPQFIGK WNIIYKSKTI KVDDGKREKD AVEITEYIEA K

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAD31744.1.
RefSeq	NP_001018770.1.
3D structure databases
HSSP	HSSP built from PDB template 1H1D based on UniProtKB P22734.
ModBase	Search...
Genome annotation databases
GeneID	3361178.
KEGG	spo:SPBPB21E7.04c.
Organism-specific databases
GeneDB_Spombe	SPBPB21E7.04c.
Phylogenomic databases
OMA	Q8NKC1. ASEDWIP.
Enzyme and pathway databases
BRENDA	2.1.1.6. 653.
Family and domain databases
InterPro	IPR002935. O-MeTrfase_3. [Graphical view]
PANTHER	PTHR10509. Methyltransf_3. 1 hit.
Pfam	PF01596. Methyltransf_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COMT2_SCHPO

Accession

Primary (citable) accession number: Q8NKC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)