Q8NKC1 (COMT2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable catechol O-methyltransferase 2
EC=2.1.1.6

Gene names

ORF Names:

SPBPB21E7.04c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Vacuole Ref.2.
Sequence similarities	Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism
Cellular component	Vacuole
Ligand	Magnesium Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	catecholamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	fungal-type vacuole Inferred from direct assay Ref.2. Source: PomBase
Molecular_function	catechol O-methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Probable catechol O-methyltransferase 2		PRO_0000318149
Sites
	Metal binding	183	1	Magnesium By similarity
	Metal binding	211	1	Magnesium By similarity
	Metal binding	212	1	Magnesium By similarity
	Binding site	78	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	100	1	S-adenosyl-L-methionine By similarity
	Binding site	108	1	S-adenosyl-L-methionine By similarity
	Binding site	127	1	S-adenosyl-L-methionine By similarity
	Binding site	128	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	156	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	183	1	S-adenosyl-L-methionine By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NKC1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 24022103FE9993B1	FASTA	281	32,123
	10 20 30 40 50 60 MFAQALHWLT TSKLRYALAL PFLFFILYTK TKKSKEQELE NYIFSLPREK LDQIRGKPDE 70 80 90 100 110 120 VINVIDEYVE QGHFLMNIGK LKGKIISEKI QQVKPKVMIE LGGYVGYSAI LFGKQLTDPS 130 140 150 160 170 180 AHYYSLEVNP KFAKIASKII DLAGLSNKVT IIVGKATDSL VELRRSLPNV IPSFEYLDFV 190 200 210 220 230 240 FIDHWKDLYV PDLRVMETLD LIGQGSIIAA DNILRPGVPE YVKYVQGSLD YRKEYDSTVS 250 260 270 280 NVNGPQFIGK WNIIYKSKTI KVDDGKREKD AVEITEYIEA K

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAD31744.1.
RefSeq	NP_001018770.1. NM_001020945.2.
3D structure databases
ProteinModelPortal	Q8NKC1.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
MINT	MINT-4700849.
STRING	4896.SPBPB21E7.04c-1.
Proteomic databases
MaxQB	Q8NKC1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBPB21E7.04c.1; SPBPB21E7.04c.1:pep; SPBPB21E7.04c.
GeneID	3361178.
KEGG	spo:SPBPB21E7.04c.
Organism-specific databases
PomBase	SPBPB21E7.04c.
Phylogenomic databases
eggNOG	COG4122.
HOGENOM	HOG000171638.
KO	K00545.
OMA	FIDHYKP.
OrthoDB	EOG7KM644.
PhylomeDB	Q8NKC1.
Family and domain databases
Gene3D	3.40.50.150. 1 hit.
InterPro	IPR025782. Catechol_O-MeTrfase. IPR002935. O-MeTrfase_3. IPR029063. SAM-dependent_MTases-like. [Graphical view]
PANTHER	PTHR10509. PTHR10509. 1 hit.
Pfam	PF01596. Methyltransf_3. 1 hit. [Graphical view]
SUPFAM	SSF53335. SSF53335. 1 hit.
PROSITE	PS51682. SAM_OMT_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20811292.

Entry information

Entry name

COMT2_SCHPO

Accession

Primary (citable) accession number: Q8NKC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 1, 2002
Last modified:	June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program