Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8NKC1 (COMT2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable catechol O-methyltransferase 2

EC=2.1.1.6
Gene names
ORF Names:SPBPB21E7.04c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subcellular location

Vacuole Ref.2.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.

Ontologies

Keywords
   Biological processCatecholamine metabolism
   Cellular componentVacuole
   LigandMagnesium
Metal-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcatecholamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentfungal-type vacuole

Inferred from direct assay Ref.2. Source: PomBase

   Molecular_functioncatechol O-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 281281Probable catechol O-methyltransferase 2
PRO_0000318149

Sites

Metal binding1831Magnesium By similarity
Metal binding2111Magnesium By similarity
Metal binding2121Magnesium By similarity
Binding site781S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1001S-adenosyl-L-methionine By similarity
Binding site1081S-adenosyl-L-methionine By similarity
Binding site1271S-adenosyl-L-methionine By similarity
Binding site1281S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1561S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1831S-adenosyl-L-methionine By similarity
Binding site1861Substrate By similarity
Binding site2121Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8NKC1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 24022103FE9993B1

FASTA28132,123
        10         20         30         40         50         60 
MFAQALHWLT TSKLRYALAL PFLFFILYTK TKKSKEQELE NYIFSLPREK LDQIRGKPDE 

        70         80         90        100        110        120 
VINVIDEYVE QGHFLMNIGK LKGKIISEKI QQVKPKVMIE LGGYVGYSAI LFGKQLTDPS 

       130        140        150        160        170        180 
AHYYSLEVNP KFAKIASKII DLAGLSNKVT IIVGKATDSL VELRRSLPNV IPSFEYLDFV 

       190        200        210        220        230        240 
FIDHWKDLYV PDLRVMETLD LIGQGSIIAA DNILRPGVPE YVKYVQGSLD YRKEYDSTVS 

       250        260        270        280 
NVNGPQFIGK WNIIYKSKTI KVDDGKREKD AVEITEYIEA K 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329671 Genomic DNA. Translation: CAD31744.1.
RefSeqNP_001018770.1. NM_001020945.2.

3D structure databases

ProteinModelPortalQ8NKC1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4700849.
STRING4896.SPBPB21E7.04c-1.

Proteomic databases

MaxQBQ8NKC1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBPB21E7.04c.1; SPBPB21E7.04c.1:pep; SPBPB21E7.04c.
GeneID3361178.
KEGGspo:SPBPB21E7.04c.

Organism-specific databases

PomBaseSPBPB21E7.04c.

Phylogenomic databases

eggNOGCOG4122.
HOGENOMHOG000171638.
KOK00545.
OMAFIDHYKP.
OrthoDBEOG7KM644.
PhylomeDBQ8NKC1.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR025782. Catechol_O-MeTrfase.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10509. PTHR10509. 1 hit.
PfamPF01596. Methyltransf_3. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20811292.

Entry information

Entry nameCOMT2_SCHPO
AccessionPrimary (citable) accession number: Q8NKC1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names