Reviewed, UniProtKB/Swiss-Prot Q8NKB8 (LEU3_ARXAD)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
      Short name=3-IPM-DH
      Short name=IMDH
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase

Gene names

Name:

LEU2

Organism

Arxula adeninivorans (Yeast)

Taxonomic identifier

37620 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › mitosporic Dipodascaceae › Arxula

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	3-isopropylmalate dehydrogenase		PRO_0000083597
Regions
	Nucleotide binding	77 – 88	12	NAD By similarity
	Nucleotide binding	287 – 298	12	NAD By similarity
Sites
	Metal binding	223	1	Magnesium or manganese By similarity
	Metal binding	248	1	Magnesium or manganese By similarity
	Metal binding	252	1	Magnesium or manganese By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity
	Site	141	1	Important for catalysis By similarity
	Site	190	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NKB8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: AB9CD5FC4CD71DB6	FASTA	362	38,833
	10 20 30 40 50 60 MSKNIIILSG DHVGPEVTAE AIKVLEAITQ ARPNVKFNFD HKLIGGAAID ATGSPLPDET 70 80 90 100 110 120 LEASKKADAV LLGAVGGPKW GTGAVRPEQG LLKIRKELNL YANLRPCNFM SEKLLDLSPL 130 140 150 160 170 180 RSEIVKGTNF TVVRELVGGI YFGTRKEDEG NGEAWDTEKY TVEEVKRITR MAAFLALQSN 190 200 210 220 230 240 PPLPVWSLDK ANVLASSRLW RKTVTETIEK EFPQLTLNHQ LIDSAAMILI QNPSKMNGVI 250 260 270 280 290 300 VTSNMFGDII SDEASVIPGS LGLLPSASLS SLPDKNTAFG LYEPCHGSAP DLPPNKVNPI 310 320 330 340 350 360 ATILSAAMML RLSLNLKEEA DAVEKAVSKV IDNGIVTADL KGASSTTEVG DAVAAEVQKL LK

References

[1]	"The ALEU2 gene -- a new component for an Arxula adeninivorans-based expression platform." Wartmann T., Stoltenburg R., Boeer E., Sieber H., Bartelsen O., Gellissen G., Kunze G. FEMS Yeast Res. 3:223-232(2003) [PubMed: 12702456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LS3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ488496 Genomic DNA. Translation: CAD32688.1.
3D structure databases
HSSP	HSSP built from PDB template 2AYQ based on UniProtKB P12010.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.85. 97311.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_ARXAD

Accession

Primary (citable) accession number: Q8NKB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)