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Q8NK90

- ABFA_ASPKW

UniProt

Q8NK90 - ABFA_ASPKW

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Protein

Alpha-L-arabinofuranosidase A

Gene

abfA

Organism
Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Acts only on small linear 1,5-alpha-linked L-arabinofuranosyl oligosaccharides.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

pH dependencei

Optimum pH is 4.0. Stable between pH 3.0 and 7.0.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius.1 Publication

Pathwayi

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: UniProtKB

GO - Biological processi

  1. arabinan catabolic process Source: UniProtKB-UniPathway
  2. arabinose metabolic process Source: UniProtKB
  3. L-arabinose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

SABIO-RKQ8NK90.
UniPathwayiUPA00667.

Protein family/group databases

CAZyiGH51. Glycoside Hydrolase Family 51.
mycoCLAPiABF51A_ASPKA.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-L-arabinofuranosidase A (EC:3.2.1.55)
Short name:
ABF A
Short name:
Arabinosidase A
Gene namesi
Name:abfA
ORF Names:AKAW_10173
OrganismiAspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Taxonomic identifieri1033177 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006812: Unassembled WGS sequence

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 628603Alpha-L-arabinofuranosidase APRO_0000394598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi260 – 2601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi359 – 3591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi493 – 4931N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 51 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ8NK90.
OrthoDBiEOG725DS5.

Family and domain databases

InterProiIPR010720. Alpha-L-AF_C.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTiSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NK90-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAFSALSGV SALSLLLCLV QHAHGVSLKV STQGGNSSSP ILYGFMFEDI
60 70 80 90 100
NHSGDGGIYG QLLQNPGLQG TTPNLTAWAA VGDATIAIDG DSPLTSAIPS
110 120 130 140 150
TIKLDVADDA TGAVGLTNEG YWGIPVDGSE FQSSFWIKGD YSGDITVRLV
160 170 180 190 200
GNYTGTEYGS ATITHTSTAD NFTQASVKFP TTKAPDGNVL YELTVDGSVA
210 220 230 240 250
AGSSLNFGYL TLFGETYKSR ENGLKPQLAN VLADMKGSFL RFPGGNNLEG
260 270 280 290 300
NSAENRWKWN ETIGDLWDRP GREGTWTYYN TDGLGLHEYF YWCEDLGLVP
310 320 330 340 350
VLGVWDGFAL ESGGNTPITG DALTPYIDDV LNELEYILGD TSTTYGAWRA
360 370 380 390 400
ANGQEEPWNL TMVEIGNEDM LGGGCESYAE RFTAFYDAIH AAYPDLILIA
410 420 430 440 450
STSEADCLPE SMPEGSWVDY HDYSTPDGLV GQFNYFDNLD RSVPYFIGEY
460 470 480 490 500
SRWEIDWPNM KGSVSEAVFM IGFERNSDVV KMAAYAPLLQ LVNSTQWTPD
510 520 530 540 550
LIGYTQSPDD IFLSTSYYVQ EMFSRNRGDT IKEVTSDSDF GPLYWVASSA
560 570 580 590 600
GDSYYVKLAN YGSETQDLTV SIPGTSTGKL TVLADNDPDA YNSDTQTLVT
610 620
PSESTVQASN GTFTFSLPAW AVAVLAAN
Length:628
Mass (Da):67,959
Last modified:March 21, 2012 - v2
Checksum:i162C5F34386D5705
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401D → E in BAB96815. (PubMed:16233515)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB085903 Genomic DNA. Translation: BAB96815.1.
DF126487 Genomic DNA. Translation: GAA92059.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB085903 Genomic DNA. Translation: BAB96815.1 .
DF126487 Genomic DNA. Translation: GAA92059.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH51. Glycoside Hydrolase Family 51.
mycoCLAPi ABF51A_ASPKA.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

InParanoidi Q8NK90.
OrthoDBi EOG725DS5.

Enzyme and pathway databases

UniPathwayi UPA00667 .
SABIO-RK Q8NK90.

Family and domain databases

InterProi IPR010720. Alpha-L-AF_C.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF06964. Alpha-L-AF_C. 1 hit.
[Graphical view ]
SMARTi SM00813. Alpha-L-AF_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Role of two alpha-L-arabinofuranosidases in arabinoxylan degradation and characteristics of the encoding genes from shochu koji molds, Aspergillus kawachii and Aspergillus awamori."
    Koseki T., Okuda M., Sudoh S., Kizaki Y., Iwano K., Aramaki I., Matsuzawa H.
    J. Biosci. Bioeng. 96:232-241(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-43 AND 482-494, SUBCELLULAR LOCATION, INDUCTION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: NBRC 4308.
  2. "Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu."
    Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.
    Eukaryot. Cell 10:1586-1587(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 4308.

Entry informationi

Entry nameiABFA_ASPKW
AccessioniPrimary (citable) accession number: Q8NK90
Secondary accession number(s): G7XYQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 21, 2012
Last modified: October 29, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3