Reviewed, UniProtKB/Swiss-Prot Q8NK50 (DCXR_TRIRE)

Names and origin

Protein names

Recommended name:
    L-xylulose reductase
      Short name=XR
    EC=1.1.1.10

Gene names

Name:

lxr1

Organism

Trichoderma reesei (Hypocrea jecorina)

Taxonomic identifier

51453 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of L-xylulose, D-xylulose, D-fructose, and L-sorbose, with the highest affinity for L-xylulose.
Catalytic activity	Xylitol + NADP+ = L-xylulose + NADPH. Ref.1
Pathway	Carbohydrate degradation; L-arabinose degradation via L-arabinitol; D-xylulose 5-phosphate from L-arabinose (fungal route): step 3/5.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Xylose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-xylulose reductase (NADP+) activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	L-xylulose reductase		PRO_0000054558
Regions
	Nucleotide binding	23 – 53	31	NADP By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Active site	178	1	By similarity
	Binding site	159	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NK50-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1CF56334DA86F109	FASTA	266	28,478
	10 20 30 40 50 60 MPQPVPTANR LLDLFSLKGK VVVVTGASGP RGMGIEAARG CAEMGADLAI TYSSRKEGAE 70 80 90 100 110 120 KNAEELTKEY GVKVKVYKVN QSDYNDVERF VNQVVSDFGK IDAFIANAGA TANSGVVDGS 130 140 150 160 170 180 ASDWDHVIQV DLSGTAYCAK AVGAHFKKQG HGSLVITASM SGHVANYPQE QTSYNVAKAG 190 200 210 220 230 240 CIHLARSLAN EWRDFARVNS ISPGYIDTGL SDFIDEKTQE LWRSMIPMGR NGDAKELKGA 250 260 YVYLVSDASS YTTGADIVID GGYTTR

References

[1]

"The missing link in the fungal L-arabinose catabolic pathway, identification of the L-xylulose reductase gene." Richard P., Putkonen M., Vaeaenaenen R., Londesborough J., Penttilae M. Biochemistry 41:6432-6437(2002) [PubMed: 12009906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF375616 mRNA. Translation: AAM20896.1.
3D structure databases
SMR	Q8NK50. Positions 2-265.
ModBase	Search...
Phylogenomic databases
PhylomeDB	Q8NK50.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13194.
BRENDA	1.1.1.10. 280374.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCXR_TRIRE

Accession

Primary (citable) accession number: Q8NK50

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)