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Protein

1,4-beta-D-glucan cellobiohydrolase B

Gene

cbhB

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.By similarity1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei235NucleophileBy similarity1
Active sitei240Proton donorBy similarity1

GO - Molecular functioni

  • cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB
  • cellulose binding Source: InterPro

GO - Biological processi

  • cellulose catabolic process Source: UniProtKB-KW
  • glucan catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH7. Glycoside Hydrolase Family 7.
mycoCLAPiCBH7B_EMENI.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-beta-D-glucan cellobiohydrolase B (EC:3.2.1.91)
Alternative name(s):
Beta-glucancellobiohydrolase B
Exocellobiohydrolase B
Exoglucanase B
Gene namesi
Name:cbhB
ORF Names:AN0494
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VIII
  • UP000005890 Componenti: Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000039355024 – 5261,4-beta-D-glucan cellobiohydrolase BAdd BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi293N-linked (GlcNAc...)Sequence analysis1
Glycosylationi400N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi498 ↔ 515By similarity
Disulfide bondi509 ↔ 525By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8NK02.

Structurei

3D structure databases

ProteinModelPortaliQ8NK02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini490 – 526CBM1PROSITE-ProRule annotationAdd BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 458CatalyticAdd BLAST435
Regioni459 – 490Ser/Thr-rich linkerAdd BLAST32

Sequence similaritiesi

Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000182210.
InParanoidiQ8NK02.
KOiK01225.
OMAiRGSCDIS.
OrthoDBiEOG092C1XF1.

Family and domain databases

CDDicd07999. GH7_CBH_EG. 1 hit.
Gene3Di2.70.100.10. 1 hit.
InterProiIPR000254. Cellulose-bd_dom_fun.
IPR013320. ConA-like_dom.
IPR001722. Glyco_hydro_7.
[Graphical view]
PfamiPF00734. CBM_1. 1 hit.
PF00840. Glyco_hydro_7. 1 hit.
[Graphical view]
PRINTSiPR00734. GLHYDRLASE7.
ProDomiPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEiPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NK02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSFQLYKA LLFFSSLLSA VQAQKVGTQQ AEVHPGLTWQ TCTSSGSCTT
60 70 80 90 100
VNGEVTIDAN WRWLHTVNGY TNCYTGNEWD TSICTSNEVC AEQCAVDGAN
110 120 130 140 150
YASTYGITTS GSSLRLNFVT QSQQKNIGSR VYLMDDEDTY TMFYLLNKEF
160 170 180 190 200
TFDVDVSELP CGLNGAVYFV SMDADGGKSR YATNEAGAKY GTGYCDSQCP
210 220 230 240 250
RDLKFINGVA NVEGWESSDT NPNGGVGNHG SCCAEMDIWE ANSISTAFTP
260 270 280 290 300
HPCDTPGQTL CTGDSCGGTY SNDRYGGTCD PDGCDFNSYR QGNKTFYGPG
310 320 330 340 350
LTVDTNSPVT VVTQFLTDDN TDTGTLSEIK RFYVQNGVVI PNSESTYPAN
360 370 380 390 400
PGNSITTEFC ESQKELFGDV DVFSAHGGMA GMGAALEQGM VLVLSLWDDN
410 420 430 440 450
YSNMLWLDSN YPTDADPTQP GIARGTCPTD SGVPSEVEAQ YPNAYVVYSN
460 470 480 490 500
IKFGPIGSTF GNGGGSGPTT TVTTSTATST TSSATSTATG QAQHWEQCGG
510 520
NGWTGPTVCA SPWACTVVNS WYSQCL
Length:526
Mass (Da):56,123
Last modified:October 1, 2002 - v1
Checksum:i9A9CD7C5BEBCB6C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420020 Genomic DNA. Translation: AAM54070.1.
AACD01000007 Genomic DNA. Translation: EAA66593.1.
BN001308 Genomic DNA. Translation: CBF89371.1.
RefSeqiXP_658098.1. XM_653006.1.

Genome annotation databases

EnsemblFungiiCADANIAT00002195; CADANIAP00002195; CADANIAG00002195.
EAA66593; EAA66593; AN0494.2.
GeneIDi2876273.
KEGGiani:AN0494.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420020 Genomic DNA. Translation: AAM54070.1.
AACD01000007 Genomic DNA. Translation: EAA66593.1.
BN001308 Genomic DNA. Translation: CBF89371.1.
RefSeqiXP_658098.1. XM_653006.1.

3D structure databases

ProteinModelPortaliQ8NK02.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH7. Glycoside Hydrolase Family 7.
mycoCLAPiCBH7B_EMENI.

Proteomic databases

PRIDEiQ8NK02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00002195; CADANIAP00002195; CADANIAG00002195.
EAA66593; EAA66593; AN0494.2.
GeneIDi2876273.
KEGGiani:AN0494.2.

Phylogenomic databases

HOGENOMiHOG000182210.
InParanoidiQ8NK02.
KOiK01225.
OMAiRGSCDIS.
OrthoDBiEOG092C1XF1.

Family and domain databases

CDDicd07999. GH7_CBH_EG. 1 hit.
Gene3Di2.70.100.10. 1 hit.
InterProiIPR000254. Cellulose-bd_dom_fun.
IPR013320. ConA-like_dom.
IPR001722. Glyco_hydro_7.
[Graphical view]
PfamiPF00734. CBM_1. 1 hit.
PF00840. Glyco_hydro_7. 1 hit.
[Graphical view]
PRINTSiPR00734. GLHYDRLASE7.
ProDomiPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEiPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBHB_EMENI
AccessioniPrimary (citable) accession number: Q8NK02
Secondary accession number(s): C8VSY4, Q5BG36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.