Q8NJY3 (Q8NJY3_9ASCO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Endoglucanase EMBL AAM77714.2

Gene names

Name:

cel12A EMBL AAM77714.2

Organism

Humicola grisea EMBL AAM77714.2

Taxonomic identifier

5527 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › mitosporic Ascomycota › Humicola

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 1W2U PDB 1OLR PDB 1UU4 PDB 1UU6 PDB 1UU5
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8NJY3 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: E034A8FFABD22207	FASTA	254	28,883
	10 20 30 40 50 60 MLKSALLLGA AAVSVQSASI PTIPANLEPR QIRSLCELYG YWSGNGYELL NNLWGKDTAT 70 80 90 100 110 120 SGWQCTYLDG TNNGGIQWST AWEWQGAPDN VKSYPYVGKQ IQRGRKISDI NSMRTSVSWT 130 140 150 160 170 180 YDRTDIRANV AYDVFTARDP DHPNWGGDYE LMIWLARYGG IYPIGTFHSQ VNLAGRTWDL 190 200 210 220 230 240 WTGYNGNMRV YSFLPPSGDI RDFSCDIKDF FNYLERNHGY PAREQNLIVY QVGTECFTGG 250 PARFTCRDFR ADLW

References

[1]	"Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase." Goedegebuur F., Fowler T., Phillips J., van der Kley P., van Solingen P., Dankmeyer L., Power S.D. Curr. Genet. 41:89-98(2002) [PubMed: 12073090] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Goedegebuur F., Fowler T., Phillips J., van der Kley P., van Solingen P., Dankmeyer L., Power S.D. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"The Humicola grisea Cel12A enzyme structure at 1.2 A resolution and the impact of its free cysteine residues on thermal stability." Sandgren M., Gualfetti P.J., Paech C., Paech S., Shaw A., Gross L.S., Saldajeno M., Berglund G.I., Jones T.A., Mitchinson C. Protein Sci. 12:2782-2793(2003) [PubMed: 14627738] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 32-254.
[4]	"Crystal complex structures reveal how substrate is bound in the -4 to the +2 binding sites of Humicola grisea Cel12A." Sandgren M., Berglund G.I., Shaw A., Stahlberg J., Kenne L., Desmet T., Mitchinson C. J. Mol. Biol. 342:1505-1517(2004) [PubMed: 15364577] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 32-254.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF435071 Genomic DNA. Translation: AAM77714.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OLR	X-ray	1.20	A	32-254	[»]
1UU4	X-ray	1.49	A	32-254	[»]
1UU5	X-ray	1.67	A	32-254	[»]
1UU6	X-ray	1.40	A	32-254	[»]
1W2U	X-ray	1.52	A	32-254	[»]

ProteinModelPortal

Q8NJY3.

SMR

Q8NJY3. Positions 31-254.

ModBase

Search...

Protein family/group databases

CAZy

GH12. Glycoside Hydrolase Family 12.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013319. Glyco_hydro_11/12_cat.
IPR002594. Glyco_hydro_12.
[Graphical view]

Gene3D

G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.

Pfam

PF01670. Glyco_hydro_12. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q8NJY3_9ASCO

Accession

Primary (citable) accession number: Q8NJY3

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	March 1, 2004
Last modified:	January 11, 2011
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)