Reviewed, UniProtKB/Swiss-Prot Q8NJS1 (ATG26_LEPMC)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26
    UDP-glycosyltransferase 51

Gene names

Name:	ATG26
Synonyms:	UGT51

Organism

Leptosphaeria maculans (Blackleg fungus)

Taxonomic identifier

5022 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Leptosphaeriaceae › Leptosphaeria › Leptosphaeria maculans complex

Protein attributes

Sequence length	1456 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity. Ref.1
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1456	1456	Sterol 3-beta-glucosyltransferase		PRO_0000215612
Regions
	Domain	236 – 283	48	GRAM 1
	Domain	287 – 384	98	PH
	Domain	774 – 840	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	Q8NJS1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 9D414C1416FCAB9C	FASTA	1,456	162,245
	10 20 30 40 50 60 MASQDRGSDR TSRRLTKKRK DGKKPMRDVS LDMPERFKDG DDAHEDVTAP KGNHTMSMNQ 70 80 90 100 110 120 SIFSMIARAG QQSNTDLGTM QEVDSGDSDD EGTRNVPYHI LDGAARLSRL STANDFQKTS 130 140 150 160 170 180 GQTRDGKTEK GKHRRALSEN KLLRSLPKLK IASRKDARSD GQGADQMSSS QFLPPRPSPE 190 200 210 220 230 240 GSMPAPASSP PPPAAKGRVR PGDGIQMEKG RNVARKTRQR SPAAAATGEA PVSLAKRIQH 250 260 270 280 290 300 IFEFAQEEEV ISEYPCWLLQ SILLQGYMYI TQKHICFYAY IPKKHHDVSK TGYLSKRGRS 310 320 330 340 350 360 KHNRYWFILR GDVLAYYTNP AELYFPRNRI NLQYAISAEV LEPKRKGDEE TSFVVTTDER 370 380 390 400 410 420 TYQFKADSVA SAREWVKSIQ KVIFRTHNEG NSVKISLPIQ NVLEIEESSI LDFAETAKVR 430 440 450 460 470 480 VIDNDETFAI DEYFFSFFTK GQDALNVLRI MINDNEHHQA AQKPVEPPSR ALGQSDSGFM 490 500 510 520 530 540 ASTNAPSEVP HITENVRATL SPLTAPHVGR SSMSDISVRS SVDANRKNRD VRRSMDAGRT 550 560 570 580 590 600 LRRWSLEGRR LSHEAHRSPS PSGHDKTHKG RVGDRSPKSP RATDSDSATF SLDPGTESSA 610 620 630 640 650 660 AIQSMDDSTA SASQILDRSD LFRAPVVSSP NAFSNGVNMS AHSQDTTRSS APRPAYSKGT 670 680 690 700 710 720 HPTTSPSIDP LSPGAPDGEY DTDAGGATRL SGSSSALQDI ASYPLQKASG LAGFLRTRSK 730 740 750 760 770 780 KMGNLLAAES MGYYEKVSGM LAGGRKHYNT AEGLETDDQV NVYEDDEDAA KATDNFREHF 790 800 810 820 830 840 AFREDEVLQS SFFASLQRVL PNYGKIYISG RYFCFRSLMP TSKTKIILPM KDIENVNKEK 850 860 870 880 890 900 GFRLGYYGLA IVIRGHEELF FEFGKAEYRD ECAITVLRIL ENTKYLEDDQ SSSSGVDSND 910 920 930 940 950 960 EAAKAEHDLL QQAREDNNVD KTKNLSEIVR AAADDRIPLI FDDPLASFVD FKPPEPLTIV 970 980 990 1000 1010 1020 CLTIGSRGDV QPYIALCKEL LKEGHKPRIA THAEFEPWVR KHGIDFAPVD GNPAELMRIC 1030 1040 1050 1060 1070 1080 VEHGMFTYNF MKEANSKFRG WLDDVCSSSW RACQGADVLI ESPSTMAGIH IAEALEIPYF 1090 1100 1110 1120 1130 1140 RAFTMPWTRT RAYPHAFSVL EKKMGGGYNS ITYITFDTIF WTAISGQINK WRRRELGLQN 1150 1160 1170 1180 1190 1200 TSQSKMQASL RPFLYNFSPH VVPPPLDWPD WVRVTGYWFL DEADTYEPPA DLVAFMDKAR 1210 1220 1230 1240 1250 1260 KDGKKLVYVG FGSIVIDDPA ALTKTVVDSV LKADVRCVLS KGWSDRLETK DASKPEIPLP 1270 1280 1290 1300 1310 1320 SEIFQIQSAP HDWLFKQMDA AVHHGGSGTT GASLRAGIPT IIKPFFGDQY FFAQRVEDMG 1330 1340 1350 1360 1370 1380 VGVWLKKVNT SVFSRALWEV TNSQRMIVKA RVLGQKIRKD NGTQVAIQTI YRELDRARSL 1390 1400 1410 1420 1430 1440 VKKHAKLDGE LSEEFEEDWT MVEDGEEIDV PHPFEVQQPV AGISQDASKM GGGSLVLGSM 1450 VLKGAQKRSP ESAYRG

References

[1]	"Characterization of neutral trehalase and UDP-glucose:sterol glycosyltransferase genes from the plant pathogenic fungus Leptosphaeria maculans." Idnurm A., Warnecke D.C., Heinz E., Howlett B.J. Physiol. Mol. Plant Pathol. 62:305-313(2003) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF522873 Genomic DNA. Translation: AAM81359.1.
3D structure databases
SMR	Q8NJS1. Positions 285-384.
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Enzyme and pathway databases
BRENDA	2.4.1.173. 21219.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. PF00201. UDPGT. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_LEPMC

Accession

Primary (citable) accession number: Q8NJS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)