Q8NJK5 (RGLA_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhamnogalacturonate lyase A
EC=4.2.2.-

Gene names

Name:

rglA

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	499 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pectinolytic enzymes consist of four classes of enzymes: pectine lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Degrades the rhamnogalacturonan I (RG-I) backbone of pectin By similarity.
Catalytic activity	Cleaves the alpha-1,4 linkage of RG-I backbone between a rhamnose and a galacturonate, thereby creating an unsaturated bond between carbons 4 and 5 of the galacturonate residue.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the polysaccharide lyase 4 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Lyase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro carbon-oxygen lyase activity, acting on polysaccharides Inferred from electronic annotation. Source: InterPro carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 499	479	Rhamnogalacturonate lyase A		PRO_0000394368
Experimental info
	Non-terminal residue	499	1

Sequences

	Sequence		Length	Mass (Da)
	Q8NJK5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0CBD6E4A4EFB0D1E	FASTA	499	53,915
	10 20 30 40 50 60 MLSKTSLLSL LSLAAGVVNA DFGITTSDSY VINANSPNSL VFTVDRGSCD ITSIVHYGTE 70 80 90 100 110 120 LQYSGKGSHI GSGLGTATVS ATKSGDYIKV TCETDTLTQY MGVHDGDRII HMATYITEEP 130 140 150 160 170 180 SIGELRFIAR LNSDVLPNEE PFGDVSNTAD GEPIEGSDVF LVDGETRSKF YSSQRFIDDQ 190 200 210 220 230 240 RHCIAGDEHR VCMILNQYET SSGGPFHRDI NSNNGGDYNS LYWYMNSGHV QLESYRMGLH 250 260 270 280 290 300 GPYSMYFSRS GTPSTDIDTS FFADLDIEGY VAESGRGTVS GTASGADSSF DWVVHWYNDD 310 320 330 340 350 360 AQYWTYTSSS GSFTSPAMKP GTYTMVYYQG EYVVATSEVT VSAGSSTSKD ISGSVETGTT 370 380 390 400 410 420 IFKIGDWDGQ PTGFRNAENQ LRMHPSDSRM SDWGPLTYTV GSSSLTDFPM AIFKSVNSPV 430 440 450 460 470 480 TIKFTATSDQ TGAATLRIRT TLSFAGGRPQ ATINDYEGSA PSAPTNLDSR GVTRGAYRGY 490 GDVYDVSVPE GTIVEGENT

References

[1]

"Expression profiling of pectinolytic genes from Aspergillus niger." de Vries R.P., Jansen J., Aguilar G., Paenicova L., Joosten J.A.E., Wulfert F., Visser J. FEBS Lett. 530:41-47(2002) [PubMed: 12387863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ489944 Genomic DNA. Translation: CAD36194.1.
3D structure databases
ProteinModelPortal	Q8NJK5.
ModBase	Search...
Protein family/group databases
CAZy	PL4. Polysaccharide Lyase Family 4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013784. Carb-bd-like_fold. IPR014766. CarboxyPept_regulatory_dom. IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR016590. Rhamnogalacturonase_B. IPR015364. RhgB_N. [Graphical view]
Gene3D	G3DSA:2.60.40.1120. CarboxyPept_regulatory. 1 hit.
Pfam	PF09284. RhgB_N. 1 hit. [Graphical view]
PIRSF	PIRSF011794. Rhamnogalacturonase_B. 1 hit.
SUPFAM	SSF49452. CBD_4. 1 hit. SSF49785. Gal_bind_like. 1 hit. SSF74650. Gal_mut_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RGLA_ASPNG

Accession

Primary (citable) accession number: Q8NJK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	October 1, 2002
Last modified:	January 11, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program