Reviewed, UniProtKB/Swiss-Prot Q8NJJ1 (MTLD_ASPNG)

Names and origin

Protein names

Recommended name:
    Mannitol-1-phosphate 5-dehydrogenase
      Short name=M1PDH
      Short name=MPDH
      Short name=MPD
    EC=1.1.1.17

Gene names

Name:

mpdA

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NAD(H)-dependent interconversion of D-fructose 6-phosphate and D-mannitol 1-phosphate in the mannitol metabolic pathway. Has a strong preference for NADH over NADPH. Required for protection of conidiospores against exogenous stresses such as high temperatures and an oxidative environment. Ref.1 Ref.2
Catalytic activity	D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH.
Subunit structure	Monomer By similarity.
Induction	Strongly induced at the onset of sporulation. Ref.1
Sequence similarities	Belongs to the mannitol dehydrogenase family.
Biophysicochemical properties	Kinetic parameters: KM=0.038 mM for D-mannitol 1-phosphate KM=0.54 mM for D-fructose 6-phosphate KM=0.005 mM for NADH KM=0.083 mM for NAD+

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	mannitol metabolic process Ref.1 Inferred from mutant phenotype. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to heat Ref.1 Inferred from mutant phenotype. Source: UniProtKB response to oxidative stress Ref.1 Inferred from mutant phenotype. Source: UniProtKB
Molecular function	NAD or NADH binding Ref.2 Inferred from direct assay. Source: UniProtKB mannitol-1-phosphate 5-dehydrogenase activity Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Mannitol-1-phosphate 5-dehydrogenase		PRO_0000371522
Regions
	Nucleotide binding	5 – 16	12	NAD By similarity
Sites
	Active site	213	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NJJ1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6CA75A87F52E2AD3	FASTA	388	43,363
	10 20 30 40 50 60 MGKKAIQFGG GNIGRGFVAE FLHKAGYEVV FVDVMDKMVE ALQQNKSYKV TEVSEEGEHT 70 80 90 100 110 120 TTITNYRAIN SKTHESDVIQ EIATADVVTC AVGPHILKFI APVIAKGIDA RTESKPVAVI 130 140 150 160 170 180 ACENAIGATD TLHGFIKQHT SQDRVESLYD RAQFANSAID RIVPQQAPNS GLDVRIEKFY 190 200 210 220 230 240 EWAVEKTPFG SVGHPDIPAI HWVDNLEPYI ERKLFTVNTS HATTAYFGHF RGKKMIADAL 250 260 270 280 290 300 EDEEIRGLVH KVLEETASLI VAKHDISEEE QKEYVKKIVS RISNPYLEDK VERVGRAPLR 310 320 330 340 350 360 KLSRKERFIG PASQLAERGM KYDSLMDAVE MALRFQNVPG DDESAELANI LNEQRAEDAT 370 380 IHLTGLDEEH PLYPAVLERV RKVQQGTK

References

[1]	"Mannitol is required for stress tolerance in Aspergillus niger conidiospores." Ruijter G.J.G., Bax M., Patel H., Flitter S.J., van de Vondervoort P.J.I., de Vries R.P., vanKuyk P.A., Visser J. Eukaryot. Cell 2:690-698(2003) [PubMed: 12912888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.
[2]	"Purification and kinetic characterization of mannitol-1-phosphate dehydrogenase from Aspergillus niger." Kiser R.C., Niehaus W.G. Jr. Arch. Biochem. Biophys. 211:613-621(1981) [PubMed: 7030216] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY081178 Genomic DNA. Translation: AAL89587.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR013118. Mannitol_DH_C. IPR000669. Mannitol_DH_core. IPR013131. Mannitol_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
PROSITE	PS00974. MANNITOL_DHGENASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTLD_ASPNG

Accession

Primary (citable) accession number: Q8NJJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)