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Q8NI77 (KI18A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein KIF18A
Alternative name(s):
Marrow stromal KIF18A
Short name=MS-KIF18A
Gene names
Name:KIF18A
ORF Names:OK/SW-cl.108
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length898 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression. Ref.5 Ref.7 Ref.10 Ref.11

Subunit structure

Interacts with CENPE and ESR1. Ref.6 Ref.11 Ref.12

Subcellular location

Cell projectionruffle. Cytoplasm. Nucleus. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome Ref.1 Ref.6 Ref.8.

Induction

By estrogen. Ref.12

Post-translational modification

Glycosylated. Ref.8

Ubiquitinated. Ref.8

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

Contains 1 kinesin motor domain.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCell projection
Cytoplasm
Cytoskeleton
Microtubule
Nucleus
   Coding sequence diversityPolymorphism
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionMotor protein
   PTMGlycoprotein
Phosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from direct assay Ref.5. Source: GOC

antigen processing and presentation of exogenous peptide antigen via MHC class II

Traceable author statement. Source: Reactome

blood coagulation

Traceable author statement. Source: Reactome

metabolic process

Inferred from direct assay Ref.5. Source: GOC

microtubule depolymerization

Inferred from direct assay Ref.5. Source: UniProtKB

microtubule-based movement

Traceable author statement. Source: Reactome

mitotic cell cycle

Traceable author statement. Source: Reactome

mitotic metaphase plate congression

Inferred from direct assay Ref.5. Source: UniProtKB

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcaveola

Inferred from direct assay Ref.6. Source: UniProtKB

cytoplasm

Inferred from direct assay Ref.6Ref.8. Source: UniProtKB

cytosol

Traceable author statement. Source: Reactome

kinesin complex

Inferred from electronic annotation. Source: InterPro

kinetochore microtubule

Inferred from direct assay Ref.5. Source: UniProtKB

microtubule cytoskeleton

Inferred from direct assay. Source: LIFEdb

microtubule organizing center

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay Ref.6Ref.5Ref.8. Source: UniProtKB

ruffle

Inferred from direct assay Ref.6. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

actin binding

Inferred from physical interaction Ref.8. Source: UniProtKB

microtubule binding

Inferred from direct assay Ref.8. Source: UniProtKB

microtubule plus-end binding

Inferred from direct assay Ref.5. Source: UniProtKB

plus-end-directed microtubule motor activity

Inferred from direct assay Ref.5. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.6. Source: UniProtKB

tubulin-dependent ATPase activity

Inferred from direct assay Ref.5. Source: UniProtKB

ubiquitin binding

Inferred from direct assay Ref.8. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ANAPC4Q9UJX52EBI-355426,EBI-2554854

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 898898Kinesin-like protein KIF18A
PRO_0000125458

Regions

Domain11 – 355345Kinesin motor
Nucleotide binding113 – 1208ATP By similarity
Coiled coil368 – 45386 Potential

Amino acid modifications

Modified residue6741Phosphoserine Ref.9

Natural variations

Natural variant2731T → A.
Corresponds to variant rs12272419 [ dbSNP | Ensembl ].
VAR_049701
Natural variant3341P → S.
Corresponds to variant rs34913484 [ dbSNP | Ensembl ].
VAR_049702
Natural variant7351I → V. Ref.1 Ref.4
Corresponds to variant rs10458896 [ dbSNP | Ensembl ].
VAR_038354

Experimental info

Sequence conflict6211A → G in BAB93508. Ref.2
Sequence conflict6391P → S in BAB93508. Ref.2

Secondary structure

.................................................... 898
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8NI77 [UniParc].

Last modified May 10, 2005. Version 2.
Checksum: 6DFD904378BF9B27

FASTA898102,281
        10         20         30         40         50         60 
MSVTEEDLCH HMKVVVRVRP ENTKEKAAGF HKVVHVVDKH ILVFDPKQEE VSFFHGKKTT 

        70         80         90        100        110        120 
NQNVIKKQNK DLKFVFDAVF DETSTQSEVF EHTTKPILRS FLNGYNCTVL AYGATGAGKT 

       130        140        150        160        170        180 
HTMLGSADEP GVMYLTMLHL YKCMDEIKEE KICSTAVSYL EVYNEQIRDL LVNSGPLAVR 

       190        200        210        220        230        240 
EDTQKGVVVH GLTLHQPKSS EEILHLLDNG NKNRTQHPTD MNATSSRSHA VFQIYLRQQD 

       250        260        270        280        290        300 
KTASINQNVR IAKMSLIDLA GSERASTSGA KGTRFVEGTN INRSLLALGN VINALADSKR 

       310        320        330        340        350        360 
KNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSVFYD DTYNTLKYAN RAKDIKSSLK 

       370        380        390        400        410        420 
SNVLNVNNHI TQYVKICNEQ KAEILLLKEK LKAYEEQKAF TNENDQAKLM ISNPQEKEIE 

       430        440        450        460        470        480 
RFQEILNCLF QNREEIRQEY LKLEMLLKEN ELKSFYQQQC HKQIEMMCSE DKVEKATGKR 

       490        500        510        520        530        540 
DHRLAMLKTR RSYLEKRREE ELKQFDENTN WLHRVEKEMG LLSQNGHIPK ELKKDLHCHH 

       550        560        570        580        590        600 
LHLQNKDLKA QIRHMMDLAC LQEQQHRQTE AVLNALLPTL RKQYCTLKEA GLSNAAFESD 

       610        620        630        640        650        660 
FKEIEHLVER KKVVVWADQT AEQPKQNDLP GISVLMTFPQ LGPVQPIPCC SSSGGTNLVK 

       670        680        690        700        710        720 
IPTEKRTRRK LMPSPLKGQH TLKSPPSQSV QLNDSLSKEL QPIVYTPEDC RKAFQNPSTV 

       730        740        750        760        770        780 
TLMKPSSFTT SFQAISSNIN SDNCLKMLCE VAIPHNRRKE CGQEDLDSTF TICEDIKSSK 

       790        800        810        820        830        840 
CKLPEQESLP NDNKDILQRL DPSSFSTKHS MPVPSMVPSY MAMTTAAKRK RKLTSSTSNS 

       850        860        870        880        890 
SLTADVNSGF AKRVRQDNSS EKHLQENKPT MEHKRNICKI NPSMVRKFGR NISKGNLR 

« Hide

References

« Hide 'large scale' references
[1]"MS-KIF18A, new kinesin; structure and cellular expression."
Luboshits G., Benayahu D.
Gene 351:19-28(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-735, SUBCELLULAR LOCATION.
Tissue: Bone marrow stroma.
[2]"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
Shichijo S., Itoh K.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon adenocarcinoma.
[3]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-735.
Tissue: Testis.
[5]"The human kinesin Kif18A is a motile microtubule depolymerase essential for chromosome congression."
Mayr M.I., Huemmer S., Bormann J., Gruener T., Adio S., Woehlke G., Mayer T.U.
Curr. Biol. 17:488-498(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"MS-KIF18A, a kinesin, is associated with estrogen receptor."
Luboshits G., Benayahu D.
J. Cell. Biochem. 100:693-702(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ESR1.
[7]"The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment."
Stumpff J., von Dassow G., Wagenbach M., Asbury C., Wordeman L.
Dev. Cell 14:252-262(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"New insights on cellular distribution, microtubule interactions and post-translational modifications of MS-KIF18A."
Zusev M., Benayahu D.
J. Cell. Physiol. 217:618-625(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, ASSOCIATION WITH MICROTUBULES, PHOSPHORYLATION, GLYCOSYLATION, UBIQUITINATION.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-674, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Kinesin-8 molecular motors: putting the brakes on chromosome oscillations."
Gardner M.K., Odde D.J., Bloom K.
Trends Cell Biol. 18:307-310(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Defects in chromosome congression and mitotic progression in KIF18A-deficient cells are partly mediated through impaired functions of CENP-E."
Huang Y., Yao Y., Xu H.-Z., Wang Z.-G., Lu L., Dai W.
Cell Cycle 8:2643-2649(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CENPE.
[12]"The regulation of MS-KIF18A expression and cross talk with estrogen receptor."
Zusev M., Benayahu D.
PLoS ONE 4:E6407-E6407(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, INTERACTION WITH ESR1.
[13]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY791349 mRNA. Translation: AAX16185.1.
AB062483 mRNA. Translation: BAB93508.1.
AL136819 mRNA. Translation: CAB66753.1.
BC048347 mRNA. Translation: AAH48347.1.
CCDSCCDS7867.1.
RefSeqNP_112494.3. NM_031217.3.
UniGeneHs.301052.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3LREX-ray2.20A/B1-355[»]
ProteinModelPortalQ8NI77.
SMRQ8NI77. Positions 10-384.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123630. 4 interactions.
IntActQ8NI77. 3 interactions.
MINTMINT-1199710.
STRING9606.ENSP00000263181.

PTM databases

PhosphoSiteQ8NI77.

Polymorphism databases

DMDM66774137.

Proteomic databases

MaxQBQ8NI77.
PaxDbQ8NI77.
PRIDEQ8NI77.

Protocols and materials databases

DNASU81930.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263181; ENSP00000263181; ENSG00000121621.
GeneID81930.
KEGGhsa:81930.
UCSCuc001msc.2. human.

Organism-specific databases

CTD81930.
GeneCardsGC11M028042.
HGNCHGNC:29441. KIF18A.
HPAHPA039312.
HPA039484.
MIM611271. gene.
neXtProtNX_Q8NI77.
PharmGKBPA134951326.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5059.
HOGENOMHOG000231436.
HOVERGENHBG052245.
InParanoidQ8NI77.
KOK10401.
OMAWLHRVEN.
OrthoDBEOG7Z95KM.
PhylomeDBQ8NI77.
TreeFamTF105231.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.
REACT_604. Hemostasis.
REACT_6900. Immune System.

Gene expression databases

BgeeQ8NI77.
CleanExHS_KIF18A.
GenevestigatorQ8NI77.

Family and domain databases

Gene3D3.40.850.10. 1 hit.
InterProIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR24115. PTHR24115. 1 hit.
PfamPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8NI77.
GeneWikiKIF18A.
GenomeRNAi81930.
NextBio72271.
PROQ8NI77.
SOURCESearch...

Entry information

Entry nameKI18A_HUMAN
AccessionPrimary (citable) accession number: Q8NI77
Secondary accession number(s): Q4VPE3, Q86VS5, Q9H0F3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: July 9, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM