Q8NI77 (KI18A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIF18A

Alternative name(s):
Marrow stromal KIF18A
Short name=MS-KIF18A

Gene names

Name:	KIF18A
ORF Names:	OK/SW-cl.108

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression. Ref.5 Ref.7 Ref.10 Ref.11
Subunit structure	Interacts with CENPE and ESR1. Ref.6 Ref.11 Ref.12
Subcellular location	Cell projection › ruffle. Cytoplasm. Nucleus. Cytoplasm › cytoskeleton › centrosome Ref.1 Ref.6 Ref.8.
Induction	By estrogen. Ref.12
Post-translational modification	Glycosylated. Ref.8 Ubiquitinated. Ref.8
Sequence similarities	Belongs to the kinesin-like protein family. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell projection Cytoplasm Cytoskeleton Microtubule Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Glycoprotein Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	M phase of mitotic cell cycle Traceable author statement. Source: Reactome antigen processing and presentation of exogenous peptide antigen via MHC class II Traceable author statement. Source: Reactome blood coagulation Traceable author statement. Source: Reactome microtubule depolymerization Inferred from direct assay Ref.5. Source: UniProtKB microtubule-based movement Traceable author statement. Source: Reactome mitotic anaphase Traceable author statement. Source: Reactome mitotic metaphase plate congression Inferred from direct assay Ref.5. Source: UniProtKB mitotic prometaphase Traceable author statement. Source: Reactome protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	caveola Inferred from direct assay Ref.6. Source: UniProtKB cytosol Traceable author statement. Source: Reactome kinetochore microtubule Inferred from direct assay Ref.5. Source: UniProtKB microtubule associated complex Inferred from electronic annotation. Source: InterPro microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.6Ref.5Ref.8. Source: UniProtKB ruffle Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule plus-end binding Inferred from direct assay Ref.5. Source: UniProtKB plus-end-directed microtubule motor activity Inferred from direct assay Ref.5. Source: UniProtKB tubulin-dependent ATPase activity Inferred from direct assay Ref.5. Source: UniProtKB ubiquitin binding Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 898

898

Kinesin-like protein KIF18A

PRO_0000125458

Regions

Domain

1 – 283

283

Kinesin-motor

Nucleotide binding

113 – 120

8

ATP By similarity

Coiled coil

368 – 453

86

Potential

Amino acid modifications

Modified residue

674

1

Phosphoserine Ref.9

Natural variations

Natural variant

273

1

T → A.
Corresponds to variant rs12272419 [ dbSNP | Ensembl ].

VAR_049701

Natural variant

334

1

P → S.
Corresponds to variant rs34913484 [ dbSNP | Ensembl ].

VAR_049702

Natural variant

735

1

I → V. Ref.1 Ref.4
Corresponds to variant rs10458896 [ dbSNP | Ensembl ].

VAR_038354

Experimental info

Sequence conflict

621

1

A → G in BAB93508. Ref.2

Sequence conflict

639

1

P → S in BAB93508. Ref.2

Secondary structure

1

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898

Helix Strand Turn

Details...

Beta strand

13 – 18

6

Helix

23 – 27

5

Beta strand

32 – 36

5

Beta strand

38 – 44

7

Beta strand

72 – 75

4

Beta strand

77 – 80

4

Helix

86 – 91

6

Helix

94 – 101

8

Turn

102 – 104

3

Beta strand

107 – 112

6

Helix

119 – 123

5

Beta strand

127 – 130

4

Helix

132 – 146

15

Turn

147 – 150

4

Beta strand

151 – 163

13

Beta strand

166 – 172

7

Beta strand

177 – 181

5

Beta strand

187 – 190

4

Helix

200 – 212

13

Beta strand

229 – 240

12

Beta strand

252 – 258

7

Helix

283 – 295

13

Helix

307 – 309

3

Helix

311 – 315

5

Turn

316 – 319

4

Beta strand

324 – 332

9

Helix

336 – 338

3

Helix

339 – 351

13

Sequences

	Sequence		Length	Mass (Da)
	Q8NI77 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 6DFD904378BF9B27	FASTA	898	102,281
	10 20 30 40 50 60 MSVTEEDLCH HMKVVVRVRP ENTKEKAAGF HKVVHVVDKH ILVFDPKQEE VSFFHGKKTT 70 80 90 100 110 120 NQNVIKKQNK DLKFVFDAVF DETSTQSEVF EHTTKPILRS FLNGYNCTVL AYGATGAGKT 130 140 150 160 170 180 HTMLGSADEP GVMYLTMLHL YKCMDEIKEE KICSTAVSYL EVYNEQIRDL LVNSGPLAVR 190 200 210 220 230 240 EDTQKGVVVH GLTLHQPKSS EEILHLLDNG NKNRTQHPTD MNATSSRSHA VFQIYLRQQD 250 260 270 280 290 300 KTASINQNVR IAKMSLIDLA GSERASTSGA KGTRFVEGTN INRSLLALGN VINALADSKR 310 320 330 340 350 360 KNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSVFYD DTYNTLKYAN RAKDIKSSLK 370 380 390 400 410 420 SNVLNVNNHI TQYVKICNEQ KAEILLLKEK LKAYEEQKAF TNENDQAKLM ISNPQEKEIE 430 440 450 460 470 480 RFQEILNCLF QNREEIRQEY LKLEMLLKEN ELKSFYQQQC HKQIEMMCSE DKVEKATGKR 490 500 510 520 530 540 DHRLAMLKTR RSYLEKRREE ELKQFDENTN WLHRVEKEMG LLSQNGHIPK ELKKDLHCHH 550 560 570 580 590 600 LHLQNKDLKA QIRHMMDLAC LQEQQHRQTE AVLNALLPTL RKQYCTLKEA GLSNAAFESD 610 620 630 640 650 660 FKEIEHLVER KKVVVWADQT AEQPKQNDLP GISVLMTFPQ LGPVQPIPCC SSSGGTNLVK 670 680 690 700 710 720 IPTEKRTRRK LMPSPLKGQH TLKSPPSQSV QLNDSLSKEL QPIVYTPEDC RKAFQNPSTV 730 740 750 760 770 780 TLMKPSSFTT SFQAISSNIN SDNCLKMLCE VAIPHNRRKE CGQEDLDSTF TICEDIKSSK 790 800 810 820 830 840 CKLPEQESLP NDNKDILQRL DPSSFSTKHS MPVPSMVPSY MAMTTAAKRK RKLTSSTSNS 850 860 870 880 890 SLTADVNSGF AKRVRQDNSS EKHLQENKPT MEHKRNICKI NPSMVRKFGR NISKGNLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MS-KIF18A, new kinesin; structure and cellular expression." Luboshits G., Benayahu D. Gene 351:19-28(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-735, SUBCELLULAR LOCATION. Tissue: Bone marrow stroma.
[2]	"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon adenocarcinoma.
[3]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-735. Tissue: Testis.
[5]	"The human kinesin Kif18A is a motile microtubule depolymerase essential for chromosome congression." Mayr M.I., Huemmer S., Bormann J., Gruener T., Adio S., Woehlke G., Mayer T.U. Curr. Biol. 17:488-498(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"MS-KIF18A, a kinesin, is associated with estrogen receptor." Luboshits G., Benayahu D. J. Cell. Biochem. 100:693-702(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ESR1.
[7]	"The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment." Stumpff J., von Dassow G., Wagenbach M., Asbury C., Wordeman L. Dev. Cell 14:252-262(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"New insights on cellular distribution, microtubule interactions and post-translational modifications of MS-KIF18A." Zusev M., Benayahu D. J. Cell. Physiol. 217:618-625(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, ASSOCIATION WITH MICROTUBULES, PHOSPHORYLATION, GLYCOSYLATION, UBIQUITINATION.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-674, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Kinesin-8 molecular motors: putting the brakes on chromosome oscillations." Gardner M.K., Odde D.J., Bloom K. Trends Cell Biol. 18:307-310(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Defects in chromosome congression and mitotic progression in KIF18A-deficient cells are partly mediated through impaired functions of CENP-E." Huang Y., Yao Y., Xu H.-Z., Wang Z.-G., Lu L., Dai W. Cell Cycle 8:2643-2649(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CENPE.
[12]	"The regulation of MS-KIF18A expression and cross talk with estrogen receptor." Zusev M., Benayahu D. PLoS ONE 4:E6407-E6407(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, INTERACTION WITH ESR1.
[13]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY791349 mRNA. Translation: AAX16185.1.
AB062483 mRNA. Translation: BAB93508.1.
AL136819 mRNA. Translation: CAB66753.1.
BC048347 mRNA. Translation: AAH48347.1.

IPI

IPI00171768.

RefSeq

NP_112494.3. NM_031217.3.

UniGene

Hs.301052.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3LRE	X-ray	2.20	A/B	1-355	[»]

ProteinModelPortal

Q8NI77.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-1199710.

STRING

9606.ENSP00000263181.

PTM databases

PhosphoSite

Q8NI77.

Polymorphism databases

DMDM

66774137.

Proteomic databases

PaxDb

Q8NI77.

PRIDE

Q8NI77.

Protocols and materials databases

DNASU

81930.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263181; ENSP00000263181; ENSG00000121621.

GeneID

81930.

KEGG

hsa:81930.

UCSC

uc001msc.2. human.

Organism-specific databases

CTD

81930.

GeneCards

GC11M028042.

HGNC

HGNC:29441. KIF18A.

HPA

HPA039312.
HPA039484.

MIM

611271. gene.

neXtProt

NX_Q8NI77.

PharmGKB

PA134951326.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5059.

HOGENOM

HOG000231436.

HOVERGEN

HBG052245.

InParanoid

Q8NI77.

KO

K10401.

OMA

KRKNQHI.

OrthoDB

EOG4QZ7K8.

Enzyme and pathway databases

Reactome

REACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.
REACT_604. Hemostasis.
REACT_6900. Immune System.

Gene expression databases

Bgee

Q8NI77.

CleanEx

HS_KIF18A.

Genevestigator

Q8NI77.

GermOnline

ENSG00000121621. Homo sapiens.

Family and domain databases

Gene3D

3.40.850.10. 1 hit.

InterPro

IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]

Pfam

PF00225. Kinesin. 1 hit.
[Graphical view]

PRINTS

PR00380. KINESINHEAVY.

SMART

SM00129. KISc. 1 hit.
[Graphical view]

SUPFAM

SSF52540. SSF52540. 1 hit.

PROSITE

PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit.
PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8NI77.

GenomeRNAi

81930.

NextBio

72271.

SOURCE

Search...

Entry information

Entry name

KI18A_HUMAN

Accession

Primary (citable) accession number: Q8NI77
Secondary accession number(s): Q4VPE3, Q86VS5, Q9H0F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	May 29, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.