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Protein

Kinesin-like protein KIF18A

Gene

KIF18A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi113 – 1208ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. actin binding Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. ATP-dependent microtubule motor activity, plus-end-directed Source: UniProtKB
  4. microtubule binding Source: UniProtKB
  5. microtubule plus-end binding Source: UniProtKB
  6. tubulin-dependent ATPase activity Source: UniProtKB
  7. ubiquitin binding Source: UniProtKB

GO - Biological processi

  1. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  2. blood coagulation Source: Reactome
  3. metabolic process Source: GOC
  4. microtubule-based movement Source: GO_Central
  5. microtubule depolymerization Source: UniProtKB
  6. mitotic cell cycle Source: Reactome
  7. mitotic metaphase plate congression Source: UniProtKB
  8. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_121399. MHC class II antigen presentation.
REACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_25201. Kinesins.
REACT_682. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF18A
Alternative name(s):
Marrow stromal KIF18A
Short name:
MS-KIF18A
Gene namesi
Name:KIF18A
ORF Names:OK/SW-cl.108
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:29441. KIF18A.

Subcellular locationi

GO - Cellular componenti

  1. caveola Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. cytosol Source: Reactome
  4. kinesin complex Source: GO_Central
  5. kinetochore microtubule Source: UniProtKB
  6. microtubule cytoskeleton Source: LIFEdb
  7. microtubule organizing center Source: UniProtKB-SubCell
  8. nucleus Source: UniProtKB
  9. ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134951326.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 898898Kinesin-like protein KIF18APRO_0000125458Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei674 – 6741Phosphoserine1 Publication

Post-translational modificationi

Glycosylated.1 Publication
Ubiquitinated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8NI77.
PaxDbiQ8NI77.
PRIDEiQ8NI77.

PTM databases

PhosphoSiteiQ8NI77.

Expressioni

Inductioni

By estrogen.1 Publication

Gene expression databases

BgeeiQ8NI77.
CleanExiHS_KIF18A.
GenevestigatoriQ8NI77.

Organism-specific databases

HPAiHPA039312.
HPA039484.

Interactioni

Subunit structurei

Interacts with CENPE and ESR1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ANAPC4Q9UJX52EBI-355426,EBI-2554854

Protein-protein interaction databases

BioGridi123630. 6 interactions.
IntActiQ8NI77. 3 interactions.
MINTiMINT-1199710.
STRINGi9606.ENSP00000263181.

Structurei

Secondary structure

1
898
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 186Combined sources
Helixi23 – 275Combined sources
Beta strandi32 – 365Combined sources
Beta strandi38 – 447Combined sources
Beta strandi72 – 754Combined sources
Beta strandi77 – 804Combined sources
Helixi86 – 916Combined sources
Helixi94 – 1018Combined sources
Turni102 – 1043Combined sources
Beta strandi107 – 1126Combined sources
Helixi119 – 1235Combined sources
Beta strandi127 – 1304Combined sources
Helixi132 – 14615Combined sources
Turni147 – 1504Combined sources
Beta strandi151 – 16313Combined sources
Beta strandi166 – 1727Combined sources
Beta strandi177 – 1815Combined sources
Beta strandi187 – 1904Combined sources
Helixi200 – 21213Combined sources
Beta strandi229 – 24012Combined sources
Beta strandi252 – 2587Combined sources
Helixi283 – 29513Combined sources
Helixi307 – 3093Combined sources
Helixi311 – 3155Combined sources
Turni316 – 3194Combined sources
Beta strandi324 – 3329Combined sources
Helixi336 – 3383Combined sources
Helixi339 – 35113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LREX-ray2.20A/B1-355[»]
ProteinModelPortaliQ8NI77.
SMRiQ8NI77. Positions 10-384.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NI77.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 355345Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili368 – 45386Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00770000120451.
HOGENOMiHOG000231436.
HOVERGENiHBG052245.
InParanoidiQ8NI77.
KOiK10401.
OMAiHRVENEM.
OrthoDBiEOG7Z95KM.
PhylomeDBiQ8NI77.
TreeFamiTF105231.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NI77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVTEEDLCH HMKVVVRVRP ENTKEKAAGF HKVVHVVDKH ILVFDPKQEE
60 70 80 90 100
VSFFHGKKTT NQNVIKKQNK DLKFVFDAVF DETSTQSEVF EHTTKPILRS
110 120 130 140 150
FLNGYNCTVL AYGATGAGKT HTMLGSADEP GVMYLTMLHL YKCMDEIKEE
160 170 180 190 200
KICSTAVSYL EVYNEQIRDL LVNSGPLAVR EDTQKGVVVH GLTLHQPKSS
210 220 230 240 250
EEILHLLDNG NKNRTQHPTD MNATSSRSHA VFQIYLRQQD KTASINQNVR
260 270 280 290 300
IAKMSLIDLA GSERASTSGA KGTRFVEGTN INRSLLALGN VINALADSKR
310 320 330 340 350
KNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSVFYD DTYNTLKYAN
360 370 380 390 400
RAKDIKSSLK SNVLNVNNHI TQYVKICNEQ KAEILLLKEK LKAYEEQKAF
410 420 430 440 450
TNENDQAKLM ISNPQEKEIE RFQEILNCLF QNREEIRQEY LKLEMLLKEN
460 470 480 490 500
ELKSFYQQQC HKQIEMMCSE DKVEKATGKR DHRLAMLKTR RSYLEKRREE
510 520 530 540 550
ELKQFDENTN WLHRVEKEMG LLSQNGHIPK ELKKDLHCHH LHLQNKDLKA
560 570 580 590 600
QIRHMMDLAC LQEQQHRQTE AVLNALLPTL RKQYCTLKEA GLSNAAFESD
610 620 630 640 650
FKEIEHLVER KKVVVWADQT AEQPKQNDLP GISVLMTFPQ LGPVQPIPCC
660 670 680 690 700
SSSGGTNLVK IPTEKRTRRK LMPSPLKGQH TLKSPPSQSV QLNDSLSKEL
710 720 730 740 750
QPIVYTPEDC RKAFQNPSTV TLMKPSSFTT SFQAISSNIN SDNCLKMLCE
760 770 780 790 800
VAIPHNRRKE CGQEDLDSTF TICEDIKSSK CKLPEQESLP NDNKDILQRL
810 820 830 840 850
DPSSFSTKHS MPVPSMVPSY MAMTTAAKRK RKLTSSTSNS SLTADVNSGF
860 870 880 890
AKRVRQDNSS EKHLQENKPT MEHKRNICKI NPSMVRKFGR NISKGNLR
Length:898
Mass (Da):102,281
Last modified:May 10, 2005 - v2
Checksum:i6DFD904378BF9B27
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti621 – 6211A → G in BAB93508 (Ref. 2) Curated
Sequence conflicti639 – 6391P → S in BAB93508 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti273 – 2731T → A.
Corresponds to variant rs12272419 [ dbSNP | Ensembl ].
VAR_049701
Natural varianti334 – 3341P → S.
Corresponds to variant rs34913484 [ dbSNP | Ensembl ].
VAR_049702
Natural varianti735 – 7351I → V.2 Publications
Corresponds to variant rs10458896 [ dbSNP | Ensembl ].
VAR_038354

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY791349 mRNA. Translation: AAX16185.1.
AB062483 mRNA. Translation: BAB93508.1.
AL136819 mRNA. Translation: CAB66753.1.
BC048347 mRNA. Translation: AAH48347.1.
CCDSiCCDS7867.1.
RefSeqiNP_112494.3. NM_031217.3.
UniGeneiHs.301052.

Genome annotation databases

EnsembliENST00000263181; ENSP00000263181; ENSG00000121621.
GeneIDi81930.
KEGGihsa:81930.
UCSCiuc001msc.2. human.

Polymorphism databases

DMDMi66774137.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY791349 mRNA. Translation: AAX16185.1.
AB062483 mRNA. Translation: BAB93508.1.
AL136819 mRNA. Translation: CAB66753.1.
BC048347 mRNA. Translation: AAH48347.1.
CCDSiCCDS7867.1.
RefSeqiNP_112494.3. NM_031217.3.
UniGeneiHs.301052.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LREX-ray2.20A/B1-355[»]
ProteinModelPortaliQ8NI77.
SMRiQ8NI77. Positions 10-384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123630. 6 interactions.
IntActiQ8NI77. 3 interactions.
MINTiMINT-1199710.
STRINGi9606.ENSP00000263181.

PTM databases

PhosphoSiteiQ8NI77.

Polymorphism databases

DMDMi66774137.

Proteomic databases

MaxQBiQ8NI77.
PaxDbiQ8NI77.
PRIDEiQ8NI77.

Protocols and materials databases

DNASUi81930.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263181; ENSP00000263181; ENSG00000121621.
GeneIDi81930.
KEGGihsa:81930.
UCSCiuc001msc.2. human.

Organism-specific databases

CTDi81930.
GeneCardsiGC11M028042.
HGNCiHGNC:29441. KIF18A.
HPAiHPA039312.
HPA039484.
MIMi611271. gene.
neXtProtiNX_Q8NI77.
PharmGKBiPA134951326.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00770000120451.
HOGENOMiHOG000231436.
HOVERGENiHBG052245.
InParanoidiQ8NI77.
KOiK10401.
OMAiHRVENEM.
OrthoDBiEOG7Z95KM.
PhylomeDBiQ8NI77.
TreeFamiTF105231.

Enzyme and pathway databases

ReactomeiREACT_121399. MHC class II antigen presentation.
REACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_25201. Kinesins.
REACT_682. Mitotic Prometaphase.

Miscellaneous databases

EvolutionaryTraceiQ8NI77.
GeneWikiiKIF18A.
GenomeRNAii81930.
NextBioi72271.
PROiQ8NI77.
SOURCEiSearch...

Gene expression databases

BgeeiQ8NI77.
CleanExiHS_KIF18A.
GenevestigatoriQ8NI77.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MS-KIF18A, new kinesin; structure and cellular expression."
    Luboshits G., Benayahu D.
    Gene 351:19-28(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-735, SUBCELLULAR LOCATION.
    Tissue: Bone marrow stroma.
  2. "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
    Shichijo S., Itoh K.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon adenocarcinoma.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-735.
    Tissue: Testis.
  5. "The human kinesin Kif18A is a motile microtubule depolymerase essential for chromosome congression."
    Mayr M.I., Huemmer S., Bormann J., Gruener T., Adio S., Woehlke G., Mayer T.U.
    Curr. Biol. 17:488-498(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "MS-KIF18A, a kinesin, is associated with estrogen receptor."
    Luboshits G., Benayahu D.
    J. Cell. Biochem. 100:693-702(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ESR1.
  7. "The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment."
    Stumpff J., von Dassow G., Wagenbach M., Asbury C., Wordeman L.
    Dev. Cell 14:252-262(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "New insights on cellular distribution, microtubule interactions and post-translational modifications of MS-KIF18A."
    Zusev M., Benayahu D.
    J. Cell. Physiol. 217:618-625(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, ASSOCIATION WITH MICROTUBULES, PHOSPHORYLATION, GLYCOSYLATION, UBIQUITINATION.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-674, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Kinesin-8 molecular motors: putting the brakes on chromosome oscillations."
    Gardner M.K., Odde D.J., Bloom K.
    Trends Cell Biol. 18:307-310(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Defects in chromosome congression and mitotic progression in KIF18A-deficient cells are partly mediated through impaired functions of CENP-E."
    Huang Y., Yao Y., Xu H.-Z., Wang Z.-G., Lu L., Dai W.
    Cell Cycle 8:2643-2649(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CENPE.
  12. "The regulation of MS-KIF18A expression and cross talk with estrogen receptor."
    Zusev M., Benayahu D.
    PLoS ONE 4:E6407-E6407(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, INTERACTION WITH ESR1.
  13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiKI18A_HUMAN
AccessioniPrimary (citable) accession number: Q8NI77
Secondary accession number(s): Q4VPE3, Q86VS5, Q9H0F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: March 4, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.